##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630996.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275455
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229398631355394	31.0	31.0	34.0	28.0	34.0
2	31.262630919750958	31.0	31.0	34.0	28.0	34.0
3	30.68692889945726	31.0	31.0	34.0	26.0	34.0
4	34.829365958141985	37.0	35.0	37.0	32.0	37.0
5	35.064936196474925	35.0	35.0	37.0	32.0	37.0
6	35.282347388865695	37.0	35.0	37.0	32.0	37.0
7	35.277769508631174	37.0	35.0	37.0	32.0	37.0
8	35.31341961481912	37.0	35.0	37.0	32.0	37.0
9	37.126379989471964	39.0	37.0	39.0	33.0	39.0
10	36.9637726670418	39.0	37.0	39.0	33.0	39.0
11	37.01601350492821	39.0	37.0	39.0	33.0	39.0
12	36.90575956145287	39.0	37.0	39.0	33.0	39.0
13	37.0056270534207	39.0	37.0	39.0	33.0	39.0
14	37.866780417854095	40.0	37.0	41.0	33.0	41.0
15	37.900978381223794	39.0	37.0	41.0	33.0	41.0
16	37.88886024940553	40.0	37.0	41.0	33.0	41.0
17	37.886126590550184	40.0	37.0	41.0	33.0	41.0
18	38.03648508830844	40.0	37.0	41.0	33.0	41.0
19	37.95829082790292	40.0	37.0	41.0	33.0	41.0
20	37.99404621444519	40.0	37.0	41.0	33.0	41.0
21	37.923541050262294	40.0	37.0	41.0	33.0	41.0
22	37.70453250077145	39.0	37.0	41.0	32.0	41.0
23	37.806861374816215	39.0	37.0	41.0	33.0	41.0
24	37.60903232832949	39.0	36.0	41.0	32.0	41.0
25	37.52205986458768	39.0	36.0	41.0	32.0	41.0
26	37.3381931712984	39.0	36.0	41.0	32.0	41.0
27	37.144960156831424	39.0	36.0	40.0	31.0	41.0
28	37.25573687172133	39.0	36.0	41.0	31.0	41.0
29	37.07934508358897	39.0	35.0	40.0	31.0	41.0
30	36.87970085857944	39.0	35.0	40.0	31.0	41.0
31	36.80202573923145	39.0	35.0	40.0	31.0	41.0
32	36.474422319435114	38.0	35.0	40.0	30.0	41.0
33	36.324201775244596	38.0	35.0	40.0	30.0	41.0
34	36.21526928173386	38.0	35.0	40.0	30.0	41.0
35	36.181815541558514	38.0	35.0	40.0	30.0	41.0
36	36.10157375977927	38.0	35.0	40.0	30.0	41.0
37	35.97108783648872	38.0	35.0	40.0	30.0	41.0
38	35.88599589769654	38.0	35.0	40.0	29.0	41.0
39	35.67065763917881	38.0	35.0	40.0	27.0	41.0
40	35.24443920059538	38.0	34.0	40.0	26.0	41.0
41	35.187772957470365	38.0	34.0	40.0	26.0	41.0
42	34.99133433773211	38.0	34.0	40.0	25.0	41.0
43	33.897003140258846	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	12.0
11	12.0
12	9.0
13	9.0
14	4.0
15	6.0
16	9.0
17	16.0
18	26.0
19	39.0
20	61.0
21	142.0
22	221.0
23	429.0
24	711.0
25	1195.0
26	1856.0
27	2777.0
28	3974.0
29	5321.0
30	6837.0
31	8623.0
32	10505.0
33	13281.0
34	16970.0
35	22311.0
36	29428.0
37	42160.0
38	59684.0
39	48816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.25330816285781	21.710987275598555	17.26960846599263	25.766096095551
2	12.306184313227206	21.49316585286163	43.024813490406785	23.175836343504383
3	14.342814615817465	30.041567588172295	36.719972409286456	18.895645386723782
4	9.418598319144687	20.84914051296945	29.622624385108274	40.109636782777585
5	13.802254451725327	36.717794195059085	30.8798170300049	18.60013432321069
6	25.953785554809315	38.00838612477537	15.35060173168031	20.687226588735
7	22.44794975585849	33.439218747163785	16.44842170227442	27.66440979470331
8	27.681472472817703	29.56526474378755	23.524350619883467	19.228912163511282
9	20.840427656059973	11.499155941986894	15.614165653191991	52.04625074876114
10	15.752482256629941	27.449492657602875	27.198634985750846	29.599390100016336
11	30.126154907335135	20.160461781416203	23.225572235029315	26.487811076219348
12	19.187163057486703	26.689295892251003	22.38804886460583	31.73549218565646
13	34.8202065673159	16.459675809115826	25.05454611461037	23.665571508957907
14	23.065473489317675	24.457352380606633	23.61946597447859	28.857708155597102
15	28.508104772104335	24.93147701076401	19.375941623858704	27.18447659327295
16	28.944836724691875	22.223956726144017	18.945744313953277	29.885462235210834
17	18.9747871703182	33.39420231979815	19.82755804033327	27.803452469550383
18	26.704543391842588	18.840826995334993	22.835308852625655	31.619320760196768
19	23.688079722640722	30.705922927519925	22.53761957488519	23.068377774954165
20	22.653427964640322	23.80969668366884	23.060390989453815	30.476484362237027
21	31.66796754460801	22.02537619574885	22.891216351128133	23.415439908515
22	20.494817665317385	38.99874752681926	22.581183859432574	17.92525094843078
23	32.185656459312774	19.055018061026303	23.321050625329	25.438274854331922
24	29.775462416728686	27.348205696030202	22.88540777985515	19.99092410738596
25	20.704652302553956	30.894701493891922	22.7895663538509	25.611079849703216
26	29.2806447514113	31.345954874661924	22.478081719337098	16.895318654589676
27	19.36722876694923	37.259443466264905	25.476030567606323	17.89729719917954
28	24.183260423662666	22.15679512080013	27.8637163965076	25.79622805902961
29	21.337423535604728	32.628560018877856	23.141710987275598	22.892305458241818
30	22.665771178595413	26.744114283639796	29.544208672922984	21.045905864841806
31	34.36459675809116	20.609899983663393	21.744023524713654	23.281479733531793
32	21.64128442032274	26.960483563558473	27.209889092592256	24.18834292352653
33	24.31286417019114	25.36094824926031	30.855493637799274	19.47069394274927
34	23.50438365613258	28.37777495416674	25.572961100724257	22.544880288976422
35	18.794721460855676	21.193298360893795	36.274890635494	23.737089542756532
36	23.081810096022945	27.17068123649961	23.490588299359242	26.2569203681182
37	20.960955509974404	25.767911274073807	28.92269154671362	24.34844166923817
38	20.995080866203192	18.77475449710479	30.73532881958941	29.49483581710261
39	27.71196747200087	19.50772358461455	26.3778112577372	26.402497685647386
40	18.50429289720644	19.838812147174675	39.822475540469405	21.83441941514948
41	23.630357045615437	21.66306656259643	24.467517380334357	30.23905901145378
42	24.132798460728612	19.04303788277577	35.46277976438983	21.36138389210579
43	23.884482038808518	19.337096803470622	32.875061262275146	23.903359895445718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.0
2	14.0
3	83.0
4	152.0
5	152.0
6	254.5
7	357.0
8	378.5
9	400.0
10	619.0
11	838.0
12	838.0
13	1250.0
14	1662.0
15	4103.5
16	6545.0
17	6510.5
18	6476.0
19	6476.0
20	5354.5
21	4233.0
22	3274.0
23	2315.0
24	2233.5
25	2152.0
26	2152.0
27	2131.5
28	2111.0
29	2090.5
30	2070.0
31	2187.0
32	2304.0
33	2304.0
34	2719.0
35	3134.0
36	3147.5
37	3161.0
38	3594.0
39	4027.0
40	4027.0
41	5058.0
42	6089.0
43	8002.0
44	9915.0
45	13187.5
46	16460.0
47	16460.0
48	21931.0
49	27402.0
50	40503.5
51	53605.0
52	39754.0
53	25903.0
54	25903.0
55	34189.0
56	42475.0
57	35403.5
58	28332.0
59	18947.0
60	9562.0
61	9562.0
62	7804.5
63	6047.0
64	4787.5
65	3528.0
66	2897.0
67	2266.0
68	2266.0
69	1773.5
70	1281.0
71	878.5
72	476.0
73	282.0
74	88.0
75	88.0
76	61.0
77	34.0
78	29.0
79	24.0
80	16.5
81	9.0
82	9.0
83	7.0
84	5.0
85	3.5
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	275455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.183532700441088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.23121759206693	13.664663919696501
2	11.01964346542523	4.227913815323737
3	4.785965709095038	2.7543518905084317
4	2.672116876726846	2.050425659363598
5	1.7372544566821846	1.6663338839374855
6	1.2433291699784264	1.4310867473816051
7	0.9556791945800688	1.2833312156250567
8	0.7058779001551796	1.0832985424116461
9	0.5185269293365127	0.8952460474487665
>10	4.116044055864653	15.655914759216568
>50	0.5431285719692669	7.209526056887696
>100	0.40308845236743496	14.56027300284983
>500	0.03595624692479467	4.711477373799713
>1k	0.022709208584080844	9.107113684630884
>5k	0.003784868097346807	4.381840954057831
>10k+	0.005677302146020211	15.317202446860648
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	16710	6.066326623223394	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	12943	4.698771124140059	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	12539	4.552104699497195	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	6658	2.4170917209707574	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	5412	1.964749233087074	RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4323	1.569403350819553	No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	3947	1.4329019259044127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3907	1.418380497721951	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2286	0.8298996206276887	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1677	0.6088108765497087	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1504	0.5460056996605617	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1413	0.5129694505454611	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1334	0.48428962988509916	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1255	0.45560980922473726	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1216	0.441451416746837	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1213	0.4403623096331524	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1011	0.3670290973117206	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	997	0.361946597447859	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	969	0.3517815977201358	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	897	0.32564302699170466	No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	866	0.3143889201502968	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	855	0.3103955274001198	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	822	0.29841534914958884	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	684	0.24831642192009584	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	659	0.23924052930605727	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	638	0.23161677951026483	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	618	0.224356065419034	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	591	0.2145541013958723	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	584	0.21201285146394147	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	577	0.20947160153201067	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	565	0.20511517307727217	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.20184785173621825	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	544	0.19749142328147973	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	530	0.19240892341761812	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	515	0.18696338784919497	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	511	0.1855112450309488	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	482	0.17498320959866404	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	465	0.1688116026211178	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	459	0.16663338839374853	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	456	0.16554428128006388	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	417	0.1513858888021637	No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT	412	0.14957071027935598	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	411	0.14920767457479442	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	400	0.14521428182461746	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	398	0.14448821041549437	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	390	0.14158392477900203	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	389	0.14122088907444047	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	376	0.13650142491514042	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	373	0.13541231780145577	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	357	0.1296037465284711	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	351	0.12742553230110182	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	349	0.12669946089197873	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.12669946089197873	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	343	0.12452124666460947	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	338	0.12270606814180174	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	324	0.11762356827794014	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	324	0.11762356827794014	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	322	0.11689749686881704	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	321	0.1165344611642555	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	313	0.11363017552776317	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	312	0.11326713982320162	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	311	0.11290410411864008	No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA	307	0.11145196130039389	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	303	0.10999981848214771	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	300	0.10891071136846309	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	300	0.10891071136846309	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	297	0.10782160425477846	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	289	0.1049173186182861	No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC	289	0.1049173186182861	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	289	0.1049173186182861	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	288	0.10455428291372457	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	288	0.10455428291372457	No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA	285	0.10346517580003993	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	283	0.10273910439091685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.6303570456154365E-4	0.0
16	0.0	0.0	0.0	3.6303570456154365E-4	0.0
17	0.0	0.0	0.0	3.6303570456154365E-4	0.0
18	0.0	0.0	0.0	7.260714091230873E-4	0.0
19	0.0	0.0	0.0	0.001089107113684631	0.0
20	0.0	0.0	0.0	0.0014521428182461746	0.0
21	0.0	0.0	0.0	0.0014521428182461746	0.0
22	0.0	0.0	0.0	0.003630357045615436	0.0
23	0.0	0.0	0.0	0.005082499863861611	0.0
24	0.0	0.0	0.0	0.009801964023161677	0.0
25	0.0	0.0	0.0	0.011254106841407853	0.0
26	0.0	0.0	0.0	0.012706249659654027	0.0
27	0.0	0.0	0.0	0.01597357100070792	0.0
28	0.0	0.0	0.0	0.03194714200141584	0.0
29	0.0	0.0	0.0	0.07551142654880108	0.0
30	0.0	0.0	0.0	0.1263364251874172	0.0
31	0.0	0.0	0.0	0.2022108874407798	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGAGT	30	8.289042E-6	37.0	3
TATAGTT	35	5.6106E-7	37.0	13
GTATACA	20	0.0018395073	37.0	2
TTACGGG	55	1.2732926E-11	37.0	3
GACCGTT	50	1.7826096E-10	36.999996	7
GCCGAGC	25	1.2311901E-4	36.999996	3
ACAGACA	25	1.2311901E-4	36.999996	17
CAGACAC	25	1.2311901E-4	36.999996	18
CGTCTTC	670	0.0	35.34328	37
CTTATAC	1960	0.0	35.112247	1
ATTACGG	60	3.45608E-11	33.916668	2
GATTACG	60	3.45608E-11	33.916668	1
TAGGACC	55	4.965841E-10	33.636364	4
CTTAGGA	55	4.965841E-10	33.636364	2
CCGTTTA	55	4.965841E-10	33.636364	27
CCGGGGC	50	7.2886905E-9	33.3	34
TTACCGG	50	7.2886905E-9	33.3	31
TTATACA	2105	0.0	32.517815	2
TTATAGT	40	1.594226E-6	32.375	12
ATAGTTA	40	1.594226E-6	32.375	14
>>END_MODULE