Basic Statistics
Measure | Value |
---|---|
Filename | ERR1630995.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 625515 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG | 10875 | 1.738567420445553 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT | 8960 | 1.4324196861785887 | No Hit |
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT | 8354 | 1.335539515439278 | TruSeq Adapter, Index 5 (95% over 21bp) |
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC | 5760 | 0.9208412268290929 | No Hit |
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC | 2675 | 0.42764761836246934 | Illumina Paired End PCR Primer 2 (95% over 23bp) |
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC | 2425 | 0.3876805512257899 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT | 1485 | 0.2374043787918755 | No Hit |
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG | 1457 | 0.23292806727256743 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1394 | 0.2228563663541242 | No Hit |
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA | 880 | 0.1406840763211114 | No Hit |
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT | 797 | 0.12741501003173386 | No Hit |
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT | 755 | 0.12070054275277171 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTATAC | 1140 | 0.0 | 31.64474 | 1 |
GGTATCA | 215 | 0.0 | 30.116278 | 1 |
CGTCTTC | 480 | 0.0 | 29.677082 | 37 |
TTATACA | 1240 | 0.0 | 29.092741 | 2 |
TATACAC | 1345 | 0.0 | 27.2342 | 3 |
GTATCAA | 895 | 0.0 | 25.631285 | 1 |
CCGTCTT | 1415 | 0.0 | 24.710247 | 37 |
CGGATCG | 45 | 1.32227E-4 | 24.666668 | 37 |
GCCGTCT | 1460 | 0.0 | 23.948631 | 36 |
GATAGTC | 55 | 1.9008623E-5 | 23.545454 | 7 |
GCTACGA | 40 | 0.001930208 | 23.125002 | 2 |
GTTTCGG | 50 | 2.7001955E-4 | 22.199999 | 30 |
CCATTAA | 70 | 5.0957897E-6 | 21.142857 | 1 |
TGCCGTC | 1690 | 0.0 | 20.579884 | 35 |
GCTCGGA | 55 | 5.139494E-4 | 20.181818 | 11 |
ATGCCGT | 1775 | 0.0 | 19.490143 | 34 |
CGAACTA | 70 | 1.2180703E-4 | 18.5 | 29 |
GCGAACT | 70 | 1.2180703E-4 | 18.5 | 28 |
TATATCG | 100 | 2.8697286E-7 | 18.499998 | 5 |
GACGATT | 50 | 0.0070313867 | 18.499998 | 14 |