FastQCFastQC Report
Fri 10 Feb 2017
ERR1630995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625515
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG108751.738567420445553No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT89601.4324196861785887No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT83541.335539515439278TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC57600.9208412268290929No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC26750.42764761836246934Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC24250.3876805512257899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14850.2374043787918755No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG14570.23292806727256743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.2228563663541242No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA8800.1406840763211114No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT7970.12741501003173386No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT7550.12070054275277171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11400.031.644741
GGTATCA2150.030.1162781
CGTCTTC4800.029.67708237
TTATACA12400.029.0927412
TATACAC13450.027.23423
GTATCAA8950.025.6312851
CCGTCTT14150.024.71024737
CGGATCG451.32227E-424.66666837
GCCGTCT14600.023.94863136
GATAGTC551.9008623E-523.5454547
GCTACGA400.00193020823.1250022
GTTTCGG502.7001955E-422.19999930
CCATTAA705.0957897E-621.1428571
TGCCGTC16900.020.57988435
GCTCGGA555.139494E-420.18181811
ATGCCGT17750.019.49014334
CGAACTA701.2180703E-418.529
GCGAACT701.2180703E-418.528
TATATCG1002.8697286E-718.4999985
GACGATT500.007031386718.49999814