FastQCFastQC Report
Fri 10 Feb 2017
ERR1630994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654460
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT123941.8937750206276929No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT105781.616294349540079TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG102191.5614399657733093No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC51870.7925618066803166No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC44230.6758243437337653RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC28750.43929346331326596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16600.2536442257739205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16320.24936588943556523No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15330.23423891452495188No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13540.206888121504752No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT13140.20077621244995875No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12730.19451150566879566No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12610.19267793295235766No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12380.18916358524585156No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT12020.1836628670965376No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA12000.18335727164379795No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG11730.1792317330318125No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA10950.16731351037496564No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10070.15386731045442045No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA9610.1468386150414082No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA9340.1427130764294227No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT8700.13293402194175352No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG8650.13217003330990434No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA8520.13018366286709654No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT8410.12850288787702838No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7990.12208538336949547No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC7960.12162699019038596No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC7820.11948782202120833No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG7280.11123674479723743No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA6900.1054304311951838No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA6870.10497203801607433No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6870.10497203801607433No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG6640.1014576903095682No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG6610.1009992971304587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15850.034.3154561
GATTACG705.456968E-1231.7142851
CGTCTTC5200.030.95192337
TTATACA17900.030.4888292
ATTACGG751.4551915E-1129.6000022
TTTGGCG250.00549463529.622
TATACAC19000.029.4052623
AGCAACG405.9362792E-527.7500022
TTACGGG856.91216E-1126.1176473
CCGTCTT16350.025.34556437
GCCGTCT16600.024.74096536
GGTATCA3250.023.338461
GTATAAC400.00193030323.1250021
GTATCAA8300.022.734941
GCAACGC502.7003794E-422.23
ATAAGAC502.7003794E-422.23
TGCCGTC19500.021.06153935
CGTTCAA450.003824073620.55555729
TCGCCAT1650.020.1818213
TCGCCGA555.139842E-420.1818181
Produced by FastQC (version 0.11.5)