##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630994.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	654460
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428443296763746	31.0	31.0	34.0	28.0	34.0
2	31.457516120160133	31.0	31.0	34.0	28.0	34.0
3	30.908400818995812	31.0	31.0	34.0	27.0	34.0
4	35.050828163676925	37.0	35.0	37.0	32.0	37.0
5	35.259670568101946	37.0	35.0	37.0	33.0	37.0
6	35.44214314091006	37.0	35.0	37.0	33.0	37.0
7	35.449303242367755	37.0	35.0	37.0	33.0	37.0
8	35.46290376799193	37.0	35.0	37.0	33.0	37.0
9	37.23562020597134	39.0	37.0	39.0	34.0	39.0
10	37.08535433792745	39.0	37.0	39.0	33.0	39.0
11	37.132292271491	39.0	37.0	39.0	33.0	39.0
12	37.07236041927696	39.0	37.0	39.0	33.0	39.0
13	37.140117043058396	39.0	37.0	39.0	33.0	39.0
14	38.249364361458305	40.0	38.0	41.0	33.0	41.0
15	38.2729945298414	40.0	38.0	41.0	33.0	41.0
16	38.241399015982644	40.0	38.0	41.0	33.0	41.0
17	38.23221740060508	40.0	38.0	41.0	33.0	41.0
18	38.26729211869328	40.0	38.0	41.0	33.0	41.0
19	38.29580570241115	40.0	38.0	41.0	34.0	41.0
20	38.300013751795376	40.0	38.0	41.0	34.0	41.0
21	38.23493108822541	40.0	38.0	41.0	34.0	41.0
22	38.18029062127555	40.0	38.0	41.0	33.0	41.0
23	38.176055068300585	40.0	38.0	41.0	33.0	41.0
24	38.14090853528099	40.0	37.0	41.0	33.0	41.0
25	38.101327812242154	40.0	37.0	41.0	33.0	41.0
26	37.96786205421263	40.0	37.0	41.0	33.0	41.0
27	37.88828958225102	40.0	37.0	41.0	33.0	41.0
28	37.85921828683189	40.0	37.0	41.0	33.0	41.0
29	37.824667664945146	40.0	37.0	41.0	33.0	41.0
30	37.75864529535801	40.0	37.0	41.0	33.0	41.0
31	37.710790575436235	40.0	37.0	41.0	32.0	41.0
32	37.59805182898879	40.0	37.0	41.0	32.0	41.0
33	37.52409772942578	40.0	37.0	41.0	32.0	41.0
34	37.47428414265196	40.0	37.0	41.0	32.0	41.0
35	37.48861351343092	40.0	37.0	41.0	32.0	41.0
36	37.50307429025456	40.0	37.0	41.0	32.0	41.0
37	37.41565871099838	40.0	36.0	41.0	31.0	41.0
38	37.35208110503316	39.0	36.0	41.0	31.0	41.0
39	37.26993246340494	39.0	36.0	41.0	31.0	41.0
40	37.14408672798949	39.0	36.0	41.0	31.0	41.0
41	37.13938972588088	39.0	36.0	41.0	31.0	41.0
42	37.09071295419124	39.0	36.0	41.0	31.0	41.0
43	36.32272866179751	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	14.0
10	11.0
11	15.0
12	14.0
13	16.0
14	15.0
15	12.0
16	10.0
17	12.0
18	17.0
19	30.0
20	67.0
21	137.0
22	279.0
23	553.0
24	897.0
25	1702.0
26	2689.0
27	3986.0
28	5832.0
29	8253.0
30	11242.0
31	14788.0
32	19195.0
33	25020.0
34	32857.0
35	43372.0
36	60327.0
37	90069.0
38	158424.0
39	174603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.693151605904106	23.246646089906182	15.728081166152247	24.332121138037465
2	13.033646059346637	22.48571341258442	41.83158634599517	22.649054182073773
3	14.347095315221708	28.239006203587692	36.17791767258503	21.235980808605568
4	11.611557620022614	19.57170797298536	36.98973199278795	31.827002414204074
5	12.045961556092045	39.18207377074229	35.74122177062005	13.030742902545612
6	27.165296580386883	39.87715062799865	18.069095131864437	14.888457659750024
7	22.996821807291507	34.74467499923601	22.72224429300492	19.536258900467562
8	23.99321578094918	36.18998869296825	22.424899917489228	17.391895608593344
9	22.84814961953366	15.636249732603979	19.577667084313784	41.937933563548576
10	13.714207132597867	29.471014271307645	33.92567918589371	22.889099410200775
11	29.66460899061822	23.533141826849615	25.93481648993063	20.867432692601533
12	18.95654432662042	27.42306634477279	29.366959019649784	24.253430308957004
13	27.846163249090853	21.03413501207102	27.80536625615011	23.314335482688016
14	21.003422669070684	23.550713565382146	26.605904104146934	28.83995966140024
15	23.78403569354888	29.297130458698774	23.41441799346026	23.504415854292088
16	24.665525776976438	28.365522721021915	23.448797481893468	23.52015402010818
17	20.46419949271155	31.742963664700667	24.81648993062983	22.97634691195795
18	22.161782232680377	26.596430645112	26.22421538367509	25.017571738532528
19	23.417015554808547	27.764722060935732	26.31085169452679	22.50741068972894
20	23.63139076490542	26.375637930507594	26.503071234300034	23.489900070286954
21	23.298750114598295	28.125935886074018	25.192677932952357	23.38263606637533
22	22.355071356538215	31.258136478929195	25.55129419674235	20.83549796779024
23	23.418237936619505	26.84900528680133	25.899520215139198	23.833236561439968
24	23.949668428933776	27.915227821410017	25.849096965437152	22.28600678421905
25	23.313418696329798	27.684808850044313	27.40610579714574	21.59566665648015
26	24.350762460654586	27.23038841182043	25.361213825138286	23.0576353023867
27	23.078721388625738	29.11621795067689	26.839837423219144	20.965223237478227
28	20.694312868624515	28.10439140665587	27.85166396724017	23.34963175747945
29	22.545915716774132	26.751520337377382	26.083641475414847	24.618922470433642
30	23.846224368181403	26.92204260000611	28.38340005500718	20.848332976805306
31	22.75784616324909	27.664181156984384	25.845429820004277	23.732542859762244
32	23.51327812242154	26.856186779940717	28.976255233322128	20.65427986431562
33	23.886104574763927	26.105644348012103	28.956238731167684	21.05201234605629
34	20.139045930996545	29.108578064358404	26.860159520826333	23.89221648381872
35	21.206643645142563	26.455551141399013	32.14573847141155	20.19206674204688
36	24.38346117409773	26.65250741068973	25.311248968615345	23.652782446597197
37	22.301744950035143	27.110289398893745	27.42107997432998	23.16688567674113
38	22.16193503040675	25.73633224337622	28.46453564770956	23.637197078507473
39	23.707789628090335	26.19686459065489	27.171714084894415	22.92363169636036
40	20.65091831433548	25.250435473520156	31.828377593741404	22.27026861840296
41	21.46838615041408	27.2077743483177	27.112275769336552	24.21156373193167
42	22.885585062494272	25.182287687559207	30.193136326131466	21.738990923815056
43	21.915930690951317	25.420040949790668	29.894722366531184	22.769305992726828
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	13.5
2	21.0
3	67.0
4	113.0
5	113.0
6	156.0
7	199.0
8	219.5
9	240.0
10	360.0
11	480.0
12	480.0
13	685.5
14	891.0
15	2230.5
16	3570.0
17	3911.0
18	4252.0
19	4252.0
20	4693.0
21	5134.0
22	6732.0
23	8330.0
24	10153.0
25	11976.0
26	11976.0
27	15000.0
28	18024.0
29	23211.0
30	28398.0
31	29492.5
32	30587.0
33	30587.0
34	33179.5
35	35772.0
36	37374.0
37	38976.0
38	42199.0
39	45422.0
40	45422.0
41	48039.0
42	50656.0
43	46246.0
44	41836.0
45	42804.5
46	43773.0
47	43773.0
48	43465.5
49	43158.0
50	43341.5
51	43525.0
52	45465.5
53	47406.0
54	47406.0
55	50451.5
56	53497.0
57	38868.5
58	24240.0
59	28663.5
60	33087.0
61	33087.0
62	26992.0
63	20897.0
64	14473.0
65	8049.0
66	6543.5
67	5038.0
68	5038.0
69	3931.5
70	2825.0
71	2446.5
72	2068.0
73	1558.0
74	1048.0
75	1048.0
76	777.5
77	507.0
78	384.5
79	262.0
80	173.5
81	85.0
82	85.0
83	71.5
84	58.0
85	46.5
86	35.0
87	23.5
88	12.0
89	12.0
90	9.0
91	6.0
92	3.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	654460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.5199520624233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57055298107068	37.130876614036964
2	11.17799796001627	10.176438625876122
3	2.957155433314972	4.038287206968963
4	1.192548166157025	2.1713894142239436
5	0.6212205589995541	1.4138965032925752
6	0.3897780892605224	1.0645607962873156
7	0.2805184558068824	0.8938430662668004
8	0.20470008106744744	0.7454350301891497
9	0.14479643797768713	0.593201422399857
>10	1.1311244853897602	10.424594419508583
>50	0.16345558874858315	5.241327003515794
>100	0.14896364886058855	13.175037628204892
>500	0.010784698560042607	3.324748666493438
>1k	0.005055327450019973	3.7187111330657494
>5k	3.3702183000133146E-4	0.7957489540929061
>10k+	0.0010110654900039944	5.091903515576952
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	12394	1.8937750206276929	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	10578	1.616294349540079	TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	10219	1.5614399657733093	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	5187	0.7925618066803166	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	4423	0.6758243437337653	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	2875	0.43929346331326596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1660	0.2536442257739205	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1632	0.24936588943556523	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1533	0.23423891452495188	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1354	0.206888121504752	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1314	0.20077621244995875	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1273	0.19451150566879566	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1261	0.19267793295235766	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1238	0.18916358524585156	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1202	0.1836628670965376	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1200	0.18335727164379795	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1173	0.1792317330318125	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	1095	0.16731351037496564	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1007	0.15386731045442045	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	961	0.1468386150414082	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	934	0.1427130764294227	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	870	0.13293402194175352	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	865	0.13217003330990434	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	852	0.13018366286709654	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	841	0.12850288787702838	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	799	0.12208538336949547	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	796	0.12162699019038596	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	782	0.11948782202120833	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	728	0.11123674479723743	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	690	0.1054304311951838	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	687	0.10497203801607433	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	687	0.10497203801607433	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	664	0.1014576903095682	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	661	0.1009992971304587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.5279772636983161E-4	0.0
4	0.0	0.0	0.0	1.5279772636983161E-4	0.0
5	0.0	0.0	0.0	1.5279772636983161E-4	0.0
6	0.0	0.0	0.0	1.5279772636983161E-4	0.0
7	0.0	0.0	0.0	1.5279772636983161E-4	0.0
8	0.0	0.0	0.0	1.5279772636983161E-4	0.0
9	0.0	0.0	0.0	1.5279772636983161E-4	0.0
10	0.0	0.0	0.0	1.5279772636983161E-4	0.0
11	0.0	0.0	0.0	3.0559545273966323E-4	0.0
12	0.0	0.0	0.0	3.0559545273966323E-4	0.0
13	0.0	0.0	0.0	3.0559545273966323E-4	0.0
14	0.0	0.0	0.0	3.0559545273966323E-4	0.0
15	0.0	0.0	0.0	3.0559545273966323E-4	0.0
16	0.0	0.0	0.0	3.0559545273966323E-4	0.0
17	0.0	0.0	0.0	4.5839317910949484E-4	0.0
18	0.0	0.0	0.0	4.5839317910949484E-4	0.0
19	0.0	0.0	0.0	6.111909054793265E-4	0.0
20	0.0	0.0	0.0	6.111909054793265E-4	0.0
21	0.0	0.0	0.0	7.639886318491581E-4	0.0
22	0.0	0.0	0.0	0.0013751795373284845	0.0
23	0.0	0.0	0.0	0.0015279772636983162	0.0
24	0.0	0.0	0.0	0.001986370442807811	0.0
25	0.0	1.5279772636983161E-4	0.0	0.0022919658955474743	0.0
26	0.0	1.5279772636983161E-4	0.0	0.0025975613482871374	0.0
27	0.0	1.5279772636983161E-4	0.0	0.003514347706506127	0.0
28	0.0	1.5279772636983161E-4	0.0	0.007639886318491581	0.0
29	0.0	1.5279772636983161E-4	0.0	0.017571738532530638	0.0
30	0.0	1.5279772636983161E-4	0.0	0.028114781652049017	0.0
31	0.0	1.5279772636983161E-4	0.0	0.05928551783149467	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1585	0.0	34.315456	1
GATTACG	70	5.456968E-12	31.714285	1
CGTCTTC	520	0.0	30.951923	37
TTATACA	1790	0.0	30.488829	2
ATTACGG	75	1.4551915E-11	29.600002	2
TTTGGCG	25	0.005494635	29.6	22
TATACAC	1900	0.0	29.405262	3
AGCAACG	40	5.9362792E-5	27.750002	2
TTACGGG	85	6.91216E-11	26.117647	3
CCGTCTT	1635	0.0	25.345564	37
GCCGTCT	1660	0.0	24.740965	36
GGTATCA	325	0.0	23.33846	1
GTATAAC	40	0.001930303	23.125002	1
GTATCAA	830	0.0	22.73494	1
GCAACGC	50	2.7003794E-4	22.2	3
ATAAGAC	50	2.7003794E-4	22.2	3
TGCCGTC	1950	0.0	21.061539	35
CGTTCAA	45	0.0038240736	20.555557	29
TCGCCAT	165	0.0	20.18182	13
TCGCCGA	55	5.139842E-4	20.181818	1
>>END_MODULE