FastQCFastQC Report
Fri 10 Feb 2017
ERR1630993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254976
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT156326.130773092369478No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG141345.543266817269076No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT90583.5524912148594376TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC77253.0296969126506026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42711.6750596134538152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38541.511514809236948No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC36971.4499403865461846No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC30341.1899159136546185RNA PCR Primer, Index 7 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20030.7855641315261045No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG18470.7243819026104418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15040.589859437751004No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13140.5153426204819277No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA12560.4925953815261044No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT11540.45259161646586343No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9990.39180158132530124No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9710.38082015562248994No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8800.34513052208835343No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8650.3392476154618474No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8540.33493348393574296No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8520.33414909638554213No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT8310.32591302710843373No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7610.2984594628514056No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6220.2439445281124498No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6060.23766942771084337No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5850.22943335843373494No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5550.2176675451807229No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5460.2141378012048193No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5450.21374560742971888No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5310.20825489457831325No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5160.20237198795180722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.1976656626506024No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4950.19413591867469882No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4890.1917827560240964No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4540.17805597389558234No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4530.17766378012048192No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4390.1721730672690763No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4320.1694277108433735No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4250.16668235441767068No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3940.15452434738955823No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3930.15413215361445784No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3910.15334776606425704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.145503890562249No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3700.1451116967871486No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3690.1447195030120482No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3690.1447195030120482No Hit
ATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCT3580.14040537148594376No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3580.14040537148594376No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3550.13922879016064257No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3510.13766001506024098No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3310.12981613955823293No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3280.12863955823293172No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3230.1266785893574297No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3110.1219722640562249No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3090.12118787650602411No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3030.11883471385542169No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2950.11569716365461848No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2920.11452058232931726No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2840.11138303212851405No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2830.11099083835341364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2820.11059864457831327No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2810.11020645080321285No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2740.10746109437751003No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2740.10746109437751003No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2740.10746109437751003No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2730.10706890060240964No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2710.10628451305220883No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2650.10393135040160642No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2620.10275476907630522No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2620.10275476907630522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCCT200.001839251137.018
ATTACGG355.608417E-737.02
AGCCACC200.001839251137.025
ATAGCCA200.001839251137.023
ATAGATA200.001839251137.019
GGATAGA200.001839251137.017
TAGATAG200.001839251137.020
TTACGGG355.608417E-737.03
GCCACCT200.001839251137.026
CAGACAC200.001839251137.018
CTTATAC17200.035.1715131
TTATACA18300.032.9562872
GATTACG401.5936093E-632.3751
CGTCTTC4000.031.91249837
TGATACA352.3810766E-531.7142872
TATACAC19300.031.5362683
TCAGAGT303.5916947E-430.8333343
GGGTGGC250.00548842429.59999818
ATCTCGC250.00548842429.59999837
ATGTGTA250.00548842429.5999984
Produced by FastQC (version 0.11.5)