##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630993.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254976
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2279312562751	31.0	31.0	34.0	28.0	34.0
2	31.26542890311245	31.0	31.0	34.0	28.0	34.0
3	30.694555566014056	31.0	31.0	34.0	26.0	34.0
4	34.833419615963855	37.0	35.0	37.0	32.0	37.0
5	35.06591600778113	35.0	35.0	37.0	32.0	37.0
6	35.28979198042169	37.0	35.0	37.0	33.0	37.0
7	35.27307275978916	37.0	35.0	37.0	32.0	37.0
8	35.29503953313253	37.0	35.0	37.0	32.0	37.0
9	37.11023390436747	39.0	37.0	39.0	33.0	39.0
10	36.92320453689759	39.0	37.0	39.0	33.0	39.0
11	36.975523186495984	39.0	37.0	39.0	33.0	39.0
12	36.83118018950803	39.0	37.0	39.0	32.0	39.0
13	36.94453203438755	39.0	37.0	39.0	33.0	39.0
14	37.75557699548193	39.0	37.0	41.0	32.0	41.0
15	37.78232853288153	39.0	37.0	41.0	32.0	41.0
16	37.80871140813253	39.0	37.0	41.0	33.0	41.0
17	37.80588369101405	39.0	37.0	41.0	33.0	41.0
18	37.96441625878514	40.0	37.0	41.0	33.0	41.0
19	37.89608825928715	40.0	37.0	41.0	33.0	41.0
20	37.91998070406626	40.0	37.0	41.0	33.0	41.0
21	37.8739999058735	40.0	37.0	41.0	33.0	41.0
22	37.59167137299197	39.0	37.0	41.0	32.0	41.0
23	37.75146680471887	39.0	37.0	41.0	33.0	41.0
24	37.605021649096386	39.0	36.0	41.0	32.0	41.0
25	37.53799181099398	39.0	36.0	41.0	32.0	41.0
26	37.4412062311747	39.0	36.0	41.0	32.0	41.0
27	37.15176330321285	39.0	36.0	41.0	31.0	41.0
28	37.278681130773094	39.0	36.0	41.0	31.0	41.0
29	37.12239975527108	39.0	36.0	40.0	31.0	41.0
30	36.86094769703815	39.0	35.0	40.0	31.0	41.0
31	36.83813378514056	39.0	35.0	40.0	31.0	41.0
32	36.48664972389558	38.0	35.0	40.0	30.0	41.0
33	36.39343702936747	38.0	35.0	40.0	30.0	41.0
34	36.325811056726906	38.0	35.0	40.0	30.0	41.0
35	36.28139511169679	38.0	35.0	40.0	30.0	41.0
36	36.162513334588354	38.0	35.0	40.0	30.0	41.0
37	35.983927899096386	38.0	35.0	40.0	29.0	41.0
38	35.82708960843374	38.0	35.0	40.0	28.0	41.0
39	35.64772370732932	38.0	34.0	40.0	27.0	41.0
40	35.25445139934739	38.0	34.0	40.0	26.0	41.0
41	35.17419286521084	38.0	34.0	40.0	26.0	41.0
42	34.96132577183735	38.0	34.0	40.0	25.0	41.0
43	33.830709556977915	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	9.0
11	13.0
12	8.0
13	11.0
14	8.0
15	21.0
16	6.0
17	8.0
18	17.0
19	26.0
20	56.0
21	114.0
22	229.0
23	373.0
24	674.0
25	1170.0
26	1766.0
27	2587.0
28	3638.0
29	4953.0
30	6439.0
31	7907.0
32	9818.0
33	12569.0
34	15819.0
35	20599.0
36	27223.0
37	39299.0
38	54274.0
39	45333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.36009663654618	23.446912650602407	16.6988265562249	25.49416415662651
2	12.294882655622489	21.73851656626506	43.158963980923694	22.807636797188753
3	13.90483810240964	31.535909262048197	36.16536458333333	18.393888052208833
4	9.420886671686747	21.16277610441767	29.360018197791167	40.05631902610442
5	12.92003953313253	38.11456764558233	30.97781752008032	17.98757530120482
6	24.933719252008032	37.87532944277108	15.69284952309237	21.498101782128515
7	21.57457956827309	33.42000815763052	16.086219879518072	28.919192394578314
8	27.157850150602407	29.304326681726906	24.603492093373493	18.93433107429719
9	21.303965863453815	11.295572916666668	15.051612700803213	52.348848519076306
10	16.146225527108435	27.50690261044177	27.724177961847392	28.622693900602407
11	31.0542953062249	19.54850652610442	23.098252384538153	26.29894578313253
12	19.613610692771086	26.358559236947794	21.96402798694779	32.06380208333333
13	35.7421875	16.64941014056225	24.79370607429719	22.814696285140563
14	22.40367720883534	24.334054969879517	23.3367061997992	29.92556162148594
15	28.255208333333332	23.97441327811245	19.51203250502008	28.258345883534137
16	28.16382718373494	21.544380647590362	18.61194779116466	31.67984437751004
17	18.884522464859437	32.905057730923694	19.81166854919679	28.398751255020084
18	27.652406501004016	16.350166290160644	23.874011671686745	32.12341553714859
19	24.939209964859437	25.74399159136546	26.42601656626506	22.89078187751004
20	22.122866465863453	23.215126129518072	22.97980986445783	31.682197540160644
21	32.37912587851406	18.337412148594378	24.50112951807229	24.78233245481928
22	19.646554969879517	35.26527986947791	22.942551455823292	22.14561370481928
23	29.927130396586342	18.05581701807229	24.22933923192771	27.787713353413658
24	24.85528049698795	24.03598770080321	27.568869226907633	23.539862575301203
25	20.848236696787147	28.989787274096386	26.669176706827308	23.492799322289155
26	28.63563629518072	19.501051079317268	28.621517319277107	23.2417953062249
27	19.292403990963855	33.718075426706825	26.765264181726906	20.224256400602407
28	25.327873995983936	19.61792482429719	27.824579568273094	27.229621611445783
29	18.813927585341368	20.24425828313253	29.887126631526108	31.0546875
30	23.590847766064257	25.35336659136546	31.967322414658632	19.08846322791165
31	28.817614206827308	17.083176455823292	21.947163654618475	32.15204568273092
32	20.250925577309236	20.621548694779115	32.953689759036145	26.1738359688755
33	23.90734814257028	19.065715988955823	31.079787901606426	25.94714796686747
34	17.208286270080322	27.405716616465863	25.77144515562249	29.614551957831324
35	18.05464043674699	20.099538780120483	43.47350338855422	18.372317394578314
36	22.075411019076306	20.336423820281123	23.940684613453815	33.647480547188756
37	21.994619101405622	25.558876129518072	29.81653175200803	22.629973017068274
38	20.23719879518072	18.655089106425706	31.750439257028113	29.35727284136546
39	27.53317959337349	20.043062876506024	25.853413654618475	26.570343875502004
40	18.67391440763052	19.395943147590362	40.785015060240966	21.145127384538153
41	24.163450677710845	22.258565512048193	24.001474648594378	29.576509161646587
42	24.347389558232933	18.553118724899598	34.78052836345382	22.318963353413654
43	24.92156124497992	18.879816139558233	32.104590235943775	24.094032379518072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.5
2	15.0
3	64.5
4	114.0
5	114.0
6	175.5
7	237.0
8	283.5
9	330.0
10	512.5
11	695.0
12	695.0
13	1082.0
14	1469.0
15	3910.0
16	6351.0
17	6221.0
18	6091.0
19	6091.0
20	4964.5
21	3838.0
22	2921.0
23	2004.0
24	1949.0
25	1894.0
26	1894.0
27	1900.0
28	1906.0
29	1888.5
30	1871.0
31	1964.0
32	2057.0
33	2057.0
34	2380.0
35	2703.0
36	2743.5
37	2784.0
38	3112.5
39	3441.0
40	3441.0
41	4374.5
42	5308.0
43	6949.0
44	8590.0
45	11566.0
46	14542.0
47	14542.0
48	18364.5
49	22187.0
50	23157.5
51	24128.0
52	39017.0
53	53906.0
54	53906.0
55	37882.0
56	21858.0
57	28117.5
58	34377.0
59	26424.5
60	18472.0
61	18472.0
62	12385.0
63	6298.0
64	4874.0
65	3450.0
66	2815.5
67	2181.0
68	2181.0
69	1706.0
70	1231.0
71	850.0
72	469.0
73	272.5
74	76.0
75	76.0
76	61.5
77	47.0
78	35.0
79	23.0
80	20.0
81	17.0
82	17.0
83	13.0
84	9.0
85	7.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	254976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.061103790160644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5635862446482	14.557056350401606
2	10.787667885280836	4.328250502008032
3	4.490625794217122	2.7026073042168677
4	2.611874645656976	2.0958835341365463
5	1.5757267697601218	1.5805409136546182
6	1.0263728959355634	1.235410391566265
7	0.8289183007174836	1.164031124497992
8	0.6568786533987605	1.0542168674698795
9	0.5493538738245586	0.9918580572289157
>10	3.9393169244003055	16.022684487951807
>50	0.5278489179097183	7.526982931726908
>100	0.3812242184903521	14.382137926706829
>500	0.033234931868389675	4.715345758032129
>1k	0.01954995992258216	9.386765813253012
>5k	0.003909991984516432	6.58218812751004
>10k+	0.003909991984516432	11.674039909638555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	15632	6.130773092369478	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	14134	5.543266817269076	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	9058	3.5524912148594376	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	7725	3.0296969126506026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4271	1.6750596134538152	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3854	1.511514809236948	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	3697	1.4499403865461846	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	3034	1.1899159136546185	RNA PCR Primer, Index 7 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2003	0.7855641315261045	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1847	0.7243819026104418	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1504	0.589859437751004	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1314	0.5153426204819277	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	1256	0.4925953815261044	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	1154	0.45259161646586343	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	999	0.39180158132530124	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	971	0.38082015562248994	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	880	0.34513052208835343	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	865	0.3392476154618474	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	854	0.33493348393574296	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	852	0.33414909638554213	No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT	831	0.32591302710843373	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	761	0.2984594628514056	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	622	0.2439445281124498	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	606	0.23766942771084337	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	585	0.22943335843373494	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	555	0.2176675451807229	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	546	0.2141378012048193	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	545	0.21374560742971888	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	531	0.20825489457831325	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	516	0.20237198795180722	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	504	0.1976656626506024	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	495	0.19413591867469882	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	489	0.1917827560240964	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	454	0.17805597389558234	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	453	0.17766378012048192	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	439	0.1721730672690763	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	432	0.1694277108433735	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	425	0.16668235441767068	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	394	0.15452434738955823	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	393	0.15413215361445784	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	391	0.15334776606425704	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	371	0.145503890562249	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	370	0.1451116967871486	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	369	0.1447195030120482	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	369	0.1447195030120482	No Hit
ATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCT	358	0.14040537148594376	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	358	0.14040537148594376	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	355	0.13922879016064257	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	351	0.13766001506024098	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	331	0.12981613955823293	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	328	0.12863955823293172	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	323	0.1266785893574297	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	311	0.1219722640562249	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	309	0.12118787650602411	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	303	0.11883471385542169	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	295	0.11569716365461848	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	292	0.11452058232931726	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	284	0.11138303212851405	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	283	0.11099083835341364	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	282	0.11059864457831327	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	281	0.11020645080321285	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	274	0.10746109437751003	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	274	0.10746109437751003	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	274	0.10746109437751003	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	273	0.10706890060240964	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	271	0.10628451305220883	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	265	0.10393135040160642	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	262	0.10275476907630522	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	262	0.10275476907630522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.921937751004016E-4	0.0
16	0.0	0.0	0.0	0.001176581325301205	0.0
17	0.0	0.0	0.0	0.0015687751004016064	0.0
18	0.0	0.0	0.0	0.00235316265060241	0.0
19	0.0	0.0	0.0	0.002745356425702811	0.0
20	0.0	0.0	0.0	0.003137550200803213	0.0
21	0.0	0.0	0.0	0.0035297439759036145	0.0
22	0.0	0.0	0.0	0.005098519076305221	0.0
23	0.0	0.0	0.0	0.007843875502008033	0.0
24	0.0	0.0	0.0	0.008628263052208835	0.0
25	0.0	0.0	0.0	0.00941265060240964	0.0
26	0.0	0.0	0.0	0.00980484437751004	0.0
27	3.921937751004016E-4	0.0	0.0	0.010981425702811244	0.0
28	3.921937751004016E-4	0.0	0.0	0.030983308232931727	0.0
29	3.921937751004016E-4	0.0	0.0	0.0729480421686747	0.0
30	3.921937751004016E-4	0.0	0.0	0.11099083835341365	0.0
31	3.921937751004016E-4	0.0	0.0	0.17256526104417672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGCCT	20	0.0018392511	37.0	18
ATTACGG	35	5.608417E-7	37.0	2
AGCCACC	20	0.0018392511	37.0	25
ATAGCCA	20	0.0018392511	37.0	23
ATAGATA	20	0.0018392511	37.0	19
GGATAGA	20	0.0018392511	37.0	17
TAGATAG	20	0.0018392511	37.0	20
TTACGGG	35	5.608417E-7	37.0	3
GCCACCT	20	0.0018392511	37.0	26
CAGACAC	20	0.0018392511	37.0	18
CTTATAC	1720	0.0	35.171513	1
TTATACA	1830	0.0	32.956287	2
GATTACG	40	1.5936093E-6	32.375	1
CGTCTTC	400	0.0	31.912498	37
TGATACA	35	2.3810766E-5	31.714287	2
TATACAC	1930	0.0	31.536268	3
TCAGAGT	30	3.5916947E-4	30.833334	3
GGGTGGC	25	0.005488424	29.599998	18
ATCTCGC	25	0.005488424	29.599998	37
ATGTGTA	25	0.005488424	29.599998	4
>>END_MODULE