ERR1630992.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1630992.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714680
Sequences flagged as poor quality	0
Sequence length	43
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	18456	2.58241450719203	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	17364	2.4296188503945824	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	14570	2.0386746515923213	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	7488	1.0477416466110707	RNA PCR Primer, Index 39 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	7346	1.027872614316897	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	4970	0.6954161302960765	RNA PCR Primer, Index 39 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1615	0.22597526165556614	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1564	0.21883920076117983	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1538	0.21520120893266914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1514	0.21184306262942856	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1338	0.18721665640566407	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	1028	0.14384059998880616	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	915	0.1280293278110483	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	848	0.1186545027145016	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	778	0.10885990933004981	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	769	0.10760060446633457	Illumina PCR Primer Index 11 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1900	0.0	33.786842	1
TTATACA	2160	0.0	30.233797	2
TATACAC	2260	0.0	29.714602	3
GACCGTT	25	0.00549497	29.6	7
CGTCTTC	990	0.0	29.338385	37
TTACGGG	60	4.318099E-8	27.749998	3
TCGCCGA	55	6.2561776E-7	26.90909	1
GATTACG	55	6.2561776E-7	26.90909	1
CCGTCTT	3015	0.0	24.728027	37
GCCGTCT	3110	0.0	23.97267	36
GTCTATA	55	1.901353E-5	23.545454	1
CGCCGAG	65	2.6804883E-6	22.76923	2
GTACTAA	50	2.7007167E-4	22.2	1
ACCGTTA	50	2.7007167E-4	22.2	8
GTATCAA	775	0.0	21.722582	1
GGACCGT	60	3.7237485E-5	21.583332	6
TGCCGTC	3530	0.0	21.172804	35
ATGCCGT	3655	0.0	20.600546	34
CGGAGAT	55	5.140477E-4	20.181818	1
TACACCC	55	5.140477E-4	20.181818	5