FastQCFastQC Report
Fri 10 Feb 2017
ERR1630991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491928
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG167733.4096453139483827No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT129542.6333121920281015RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC91561.8612479875103676No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT87551.7797319932998323No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC48340.9826641297100388RNA PCR Primer, Index 47 (95% over 23bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC43760.8895610739783057No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG22040.4480330454863313No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT13450.27341399554406337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13290.2701614870468849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.26873851457936937No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA12940.26304662470930706No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11790.23966921988583695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5570.11322795205802474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12800.033.0976561
CGTCTTC6500.031.87692337
CTGTTCG303.5965125E-430.83333233
TTATACA14600.029.1438352
TATACAC15950.027.1410683
CGCTATC358.862154E-426.4285720
CCGTCTT21650.025.89145537
GCCGTCT22150.025.30699736
GGTATCA2200.025.2272721
GTTCGCA451.321709E-424.66666835
GCGCTAT400.001929625323.12500219
GTATCAA5600.022.7946411
CTATGCT502.6990587E-422.1999999
GTACTAA603.7207115E-521.5833321
CGCGATA705.092137E-621.14285734
TGCCGTC26950.020.86827535
TCGCAGC450.00382274320.55555737
TGTTCGC450.00382274320.55555734
TAGGCGC450.00382274320.55555716
CTCTAAC450.00382274320.5555571
Produced by FastQC (version 0.11.5)