FastQCFastQC Report
Fri 10 Feb 2017
ERR1630990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905795
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT138441.5283811458442584No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG111361.2294172522480253No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT106501.1757627277695284TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC53970.595830182326023No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC39610.43729541452536175RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC31440.3470984052683002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18540.20468207486241366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.19916206205598397No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG12180.13446751196462775No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA12010.13259070761044164No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT9630.10631544665183623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17200.032.267441
TTACCGG454.0070245E-628.77777731
TATACAC20550.028.3576663
TTATACA20200.028.1163352
CGTCTTC6400.028.03906437
CGCCGTT509.088311E-625.90000225
GACCGTT751.3729732E-824.6666667
CCGTCTT17500.024.52571537
GCCGTCT17550.024.45584136
CTATCGC400.001930872623.1250029
GATTACG802.7241185E-823.1250021
CCGTTTA652.6818052E-622.7692327
TTACGGC502.7014897E-422.218
ACGCGCC502.7014897E-422.233
GGTATCA4750.021.8105261
TACCGGG603.7251644E-521.58333232
TAGGACC1400.021.1428594
GTATCAA11500.020.5913031
TGCCGTC20950.020.57517835
TATACGA555.141938E-420.1818185
Produced by FastQC (version 0.11.5)