FastQCFastQC Report
Fri 10 Feb 2017
ERR1630989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773848
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT156992.0286929732970815No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG138191.7857512069553712No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT100131.293923354457206Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC74580.9637551560513176No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC35290.45603270926590234No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC32070.4144224705626945Illumina PCR Primer Index 11 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG18280.23622210046417383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15110.1952579834799599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.1844031386008622No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA13040.16850854431361198No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT9730.12573528651621507No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT9360.12095398579566013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20900.032.574161
TTATACA21950.031.2687912
GATTACG601.2696546E-930.8333341
TATACAC23000.029.760873
CGTCTTC4900.029.4489837
TTACGGG706.5847416E-926.428573
CGGTAAT358.8667666E-426.4285724
CCGTCTT15250.024.99016437
GCCGTCT16050.023.74454936
GTATCAA10250.021.6585371
GGTATCA3950.021.0759491
GCGGTAA450.00382469520.55555523
TGCCGTC20100.018.96019735
CGCGATC500.00703285418.528
TCTATAC1002.8719114E-718.53
TACACAT37650.018.3280225
ATACACA38050.018.1353474
ATGCCGT21000.018.05952334
GTTCTAT1005.8727946E-616.6500021
GTATAGA1352.2158929E-816.4444431
Produced by FastQC (version 0.11.5)