##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630989.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 773848 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.473993600810495 31.0 31.0 34.0 28.0 34.0 2 31.48684108507097 31.0 31.0 34.0 28.0 34.0 3 30.965569465838254 31.0 31.0 34.0 27.0 34.0 4 35.08503737168023 37.0 35.0 37.0 32.0 37.0 5 35.28956461734087 37.0 35.0 37.0 33.0 37.0 6 35.47548226525106 37.0 35.0 37.0 33.0 37.0 7 35.47942748446724 37.0 35.0 37.0 33.0 37.0 8 35.501864707281015 37.0 35.0 37.0 33.0 37.0 9 37.279048340242525 39.0 37.0 39.0 34.0 39.0 10 37.10282639484757 39.0 37.0 39.0 33.0 39.0 11 37.157222090126226 39.0 37.0 39.0 33.0 39.0 12 37.08887533469105 39.0 37.0 39.0 33.0 39.0 13 37.16580517104134 39.0 37.0 39.0 33.0 39.0 14 38.29886360112064 40.0 38.0 41.0 34.0 41.0 15 38.31441575089681 40.0 38.0 41.0 34.0 41.0 16 38.30290186186434 40.0 38.0 41.0 33.0 41.0 17 38.26755900383538 40.0 38.0 41.0 33.0 41.0 18 38.32102557608212 40.0 38.0 41.0 34.0 41.0 19 38.342038746627246 40.0 38.0 41.0 34.0 41.0 20 38.33986390092111 40.0 38.0 41.0 34.0 41.0 21 38.31323076366418 40.0 38.0 41.0 34.0 41.0 22 38.23006326823872 40.0 38.0 41.0 34.0 41.0 23 38.250452285203295 40.0 38.0 41.0 34.0 41.0 24 38.196450724173225 40.0 38.0 41.0 33.0 41.0 25 38.15285947627958 40.0 37.0 41.0 33.0 41.0 26 38.08082078134207 40.0 37.0 41.0 33.0 41.0 27 37.98853650846161 40.0 37.0 41.0 33.0 41.0 28 37.99815726085743 40.0 37.0 41.0 33.0 41.0 29 37.93071119909853 40.0 37.0 41.0 33.0 41.0 30 37.852020034942264 40.0 37.0 41.0 33.0 41.0 31 37.84557690916046 40.0 37.0 41.0 33.0 41.0 32 37.7354234940195 40.0 37.0 41.0 33.0 41.0 33 37.702177947090384 40.0 37.0 41.0 33.0 41.0 34 37.65043651983335 40.0 37.0 41.0 32.0 41.0 35 37.62430218854349 40.0 37.0 41.0 32.0 41.0 36 37.62890128293928 40.0 37.0 41.0 32.0 41.0 37 37.573271495177345 40.0 37.0 41.0 32.0 41.0 38 37.52306266863777 40.0 37.0 41.0 32.0 41.0 39 37.45509841726024 40.0 37.0 41.0 32.0 41.0 40 37.33574681332768 39.0 37.0 41.0 31.0 41.0 41 37.308471431082076 39.0 36.0 41.0 31.0 41.0 42 37.26469926910711 39.0 36.0 41.0 31.0 41.0 43 36.562408896837624 39.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 9.0 10 12.0 11 18.0 12 14.0 13 12.0 14 13.0 15 18.0 16 10.0 17 13.0 18 22.0 19 25.0 20 72.0 21 142.0 22 254.0 23 495.0 24 950.0 25 1613.0 26 2688.0 27 4488.0 28 6388.0 29 8985.0 30 12692.0 31 16937.0 32 22049.0 33 28655.0 34 37616.0 35 51236.0 36 70832.0 37 104581.0 38 185694.0 39 217315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.2882013005138 22.723196286609255 14.840898987914938 25.14770342496201 2 13.703853986829456 21.636936452636693 40.92263596985454 23.736573590679306 3 14.385641624711829 28.157596840723244 34.344212300089936 23.11254923447499 4 11.666502982497855 19.269417249899202 37.05094540529923 32.01313436230371 5 12.520546670664007 37.95680805532869 35.77575958069285 13.74688569331445 6 28.015320838200786 39.44909077751703 17.729579969192915 14.806008415089266 7 24.39070721898874 33.35978124903082 22.373127539258356 19.876383992722086 8 24.734960870868697 35.13622830323268 22.485811167050894 17.64299965884773 9 23.679714879407843 15.041972066865844 19.406911951701108 41.8714011020252 10 14.410581923064994 28.519295779016034 33.79436271722612 23.27575958069285 11 31.639676008725225 22.469270451044647 25.264780680443703 20.626272859786418 12 20.27620411243552 26.0078206572867 29.281202510053657 24.434772720224128 13 29.24501969379 20.356064756903162 26.63197423783482 23.76694131147202 14 21.475664471575815 22.076299221552553 25.992830633406044 30.455205673465592 15 23.81229905614539 28.460757151275185 22.39186506910918 25.335078723470243 16 25.1911228044784 27.02378244823273 23.28855279072893 24.496541956559945 17 20.836650091490835 30.77413135396099 24.808360298146408 23.580858256401775 18 23.981582946521797 25.484591289245433 25.725336241742564 24.808489522490206 19 23.20248937775894 28.022686625797316 26.374688569331443 22.4001354271123 20 22.17528506890242 27.200949023580854 25.123796921359233 25.49996898615749 21 24.78148163463626 25.46998893839617 25.350456420382294 24.39807300658527 22 22.52858442484829 30.450424372745033 25.256897995471977 21.764093206934696 23 23.122111835916098 25.138399272208495 25.922920263410905 25.816568628464502 24 24.211214605452234 27.331982508192826 27.11721164879925 21.339591237555698 25 22.95799691929164 28.2366821391281 25.17626200494154 23.62905893663872 26 23.75647413962432 25.68553514385254 27.104030765731775 23.45395995079137 27 22.746715117180635 30.392790315410778 25.36583411729435 21.49466045011423 28 21.132832287477644 25.13684858008291 26.918593832380523 26.811725300058924 29 23.583959640652946 25.60244389078992 28.488540385191925 22.325056083365208 30 22.33849541512028 27.14137660108962 26.807590121057366 23.712537862732734 31 22.28047368475463 24.79298260123435 26.800999679523628 26.12554403448739 32 26.25618984606796 25.880405454301105 26.362153807982963 21.50125089164797 33 21.287772275692383 25.185178484663655 27.864386804643804 25.662662435000154 34 21.77934167950295 27.708671470366276 28.006533582822467 22.505453267308308 35 23.659168208743836 25.12250467792125 29.88571399034436 21.332613122990562 36 23.20455696725972 25.517543496914126 24.898817338805554 26.379082197020605 37 22.38230246766807 26.81327599218451 27.643154728060292 23.161266812087128 38 22.169599197775273 24.936292398507202 28.13898853521622 24.755119868501307 39 24.431929784660554 25.583318687907703 26.187054822135615 23.797696705296133 40 21.507582884494113 24.73056724317954 31.515103741303204 22.246746131023144 41 22.772043088565198 26.05356607499147 25.80144938024005 25.372941456203286 42 23.190213065098057 24.901918723056724 29.292186579276553 22.61568163256867 43 23.2280757978311 24.52574665825847 29.035805481179764 23.210372062730666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 13.0 2 22.0 3 48.5 4 75.0 5 75.0 6 118.0 7 161.0 8 163.0 9 165.0 10 241.5 11 318.0 12 318.0 13 490.0 14 662.0 15 1550.0 16 2438.0 17 2923.5 18 3409.0 19 3409.0 20 3712.0 21 4015.0 22 5208.5 23 6402.0 24 8660.5 25 10919.0 26 10919.0 27 14058.0 28 17197.0 29 20601.5 30 24006.0 31 27720.5 32 31435.0 33 31435.0 34 35540.0 35 39645.0 36 42562.0 37 45479.0 38 48486.0 39 51493.0 40 51493.0 41 52833.0 42 54173.0 43 55388.0 44 56603.0 45 56541.5 46 56480.0 47 56480.0 48 55885.5 49 55291.0 50 54493.0 51 53695.0 52 65197.0 53 76699.0 54 76699.0 55 59341.5 56 41984.0 57 47076.0 58 52168.0 59 43454.0 60 34740.0 61 34740.0 62 27002.5 63 19265.0 64 16245.0 65 13225.0 66 11067.0 67 8909.0 68 8909.0 69 7238.0 70 5567.0 71 4369.0 72 3171.0 73 2516.0 74 1861.0 75 1861.0 76 1453.5 77 1046.0 78 810.0 79 574.0 80 444.5 81 315.0 82 315.0 83 230.5 84 146.0 85 98.0 86 50.0 87 39.5 88 29.0 89 29.0 90 17.5 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 773848.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.2913141202391 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26984805097347 48.99865937345342 2 11.64844216947443 14.045997717024447 3 3.1487494505368523 5.69526726624743 4 1.2833353737461526 3.0949590456057496 5 0.7019528749137184 2.116083063951395 6 0.4148145459866773 1.5005828456236276 7 0.26620799404639095 1.1235020853258788 8 0.2148521268842057 1.0362973657101693 9 0.14591600050666043 0.7917720679544429 >10 0.8260519366369503 9.189180142304041 >50 0.05720429349276404 2.3734419101923154 >100 0.019636951113226685 1.9313625341448106 >500 8.537804824342536E-4 0.40794371200516993 >1k 0.0012806707236513804 1.6479896454063743 >5k 2.134451206085634E-4 0.9597615785913431 >10k+ 6.403353618256902E-4 5.087199646459424 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT 15699 2.0286929732970815 No Hit ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG 13819 1.7857512069553712 No Hit TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT 10013 1.293923354457206 Illumina PCR Primer Index 11 (95% over 22bp) ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC 7458 0.9637551560513176 No Hit CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC 3529 0.45603270926590234 No Hit CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC 3207 0.4144224705626945 Illumina PCR Primer Index 11 (95% over 23bp) CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG 1828 0.23622210046417383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.1952579834799599 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1427 0.1844031386008622 No Hit TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA 1304 0.16850854431361198 No Hit TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT 973 0.12573528651621507 No Hit ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT 936 0.12095398579566013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.584486875975644E-4 0.0 10 0.0 0.0 0.0 2.584486875975644E-4 0.0 11 0.0 0.0 0.0 6.46121718993911E-4 0.0 12 0.0 0.0 0.0 6.46121718993911E-4 0.0 13 0.0 0.0 0.0 6.46121718993911E-4 0.0 14 0.0 0.0 0.0 6.46121718993911E-4 0.0 15 0.0 0.0 0.0 6.46121718993911E-4 0.0 16 0.0 0.0 0.0 6.46121718993911E-4 0.0 17 0.0 0.0 0.0 9.045704065914754E-4 0.0 18 0.0 0.0 0.0 9.045704065914754E-4 0.0 19 0.0 0.0 0.0 9.045704065914754E-4 0.0 20 0.0 0.0 0.0 0.0010337947503902575 0.0 21 0.0 0.0 0.0 0.0011630190941890398 0.0 22 0.0 0.0 0.0 0.001292243437987822 0.0 23 0.0 0.0 0.0 0.0016799164693841685 0.0 24 0.0 0.0 0.0 0.0018091408131829508 0.0 25 0.0 0.0 0.0 0.002067589500780515 0.0 26 0.0 0.0 0.0 0.0021968138445792973 0.0 27 0.0 0.0 0.0 0.003230608594969555 0.0 28 0.0 0.0 0.0 0.008787255378317189 0.0 29 0.0 0.0 0.0 0.018479081163225854 0.0 30 0.0 0.0 0.0 0.03385677807528093 0.0 31 0.0 0.0 0.0 0.07262408121491559 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 2090 0.0 32.57416 1 TTATACA 2195 0.0 31.268791 2 GATTACG 60 1.2696546E-9 30.833334 1 TATACAC 2300 0.0 29.76087 3 CGTCTTC 490 0.0 29.44898 37 TTACGGG 70 6.5847416E-9 26.42857 3 CGGTAAT 35 8.8667666E-4 26.42857 24 CCGTCTT 1525 0.0 24.990164 37 GCCGTCT 1605 0.0 23.744549 36 GTATCAA 1025 0.0 21.658537 1 GGTATCA 395 0.0 21.075949 1 GCGGTAA 45 0.003824695 20.555555 23 TGCCGTC 2010 0.0 18.960197 35 CGCGATC 50 0.007032854 18.5 28 TCTATAC 100 2.8719114E-7 18.5 3 TACACAT 3765 0.0 18.328022 5 ATACACA 3805 0.0 18.135347 4 ATGCCGT 2100 0.0 18.059523 34 GTTCTAT 100 5.8727946E-6 16.650002 1 GTATAGA 135 2.2158929E-8 16.444443 1 >>END_MODULE