FastQCFastQC Report
Fri 10 Feb 2017
ERR1630988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144090
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT79535.5194669997917964RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG69694.836560483031438No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT62834.360469151224929No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC39052.71011173572073No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC26221.8196960233187593No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC26171.8162259698799361RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23181.6087167742383235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17881.2408911097230897No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG9570.6641682281907141No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8930.6197515441737802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7610.5281421333888542No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6850.4753973211187452No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5850.4059962523422861No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5410.375459782080644No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5350.37129571795405647No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4810.33381914081476854No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4350.30189464917759734No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3450.2394336872787841No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3430.2380456659032549No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3340.2317995697133736No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3280.22763550558678605No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3180.2206953987091401No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3120.21653133458255255No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT2830.19640502463737944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.17836074675550004No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2440.16933860781456034No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2340.16239850093691444No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2330.16170449024914985No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2260.1568464154347977No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2200.15268235130821015No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2120.14713026580609342No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2080.14435422305503504No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2030.14088416961621208No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1960.13602609480185995No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1800.12492192379762648No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1770.12283989173433271No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1760.12214588104656812No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1700.11798181691998057No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1690.11728780623221598No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.11381775279339303No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1500.10410160316468875No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA1500.10410160316468875No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1490.10340759247692415No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1460.10132556041363038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG200.00183659737.023
TGGGGAC200.00183659737.03
GAGCCCG200.00183659737.022
CCGCGTG200.00183659737.037
TGCCGCG200.00183659737.035
ATGCCGC251.2282727E-436.99999634
CTTATAC7150.034.4125861
CGTCTTC3350.032.5820937
TTATACA7600.032.3752
CATGCCG352.37394E-531.71428533
GGTATCA2300.031.3695661
GATGTCC303.5840058E-430.83333426
TGTCCTG303.5840058E-430.83333428
TATACAC8150.030.1901843
CCGGGGA250.005480595429.59999826
GAAGCAA250.005480595429.59999831
CATATAG250.005480595429.5999983
TGTGGGG250.005480595429.5999981
CCAGCAA250.005480595429.59999830
CCATGCC250.005480595429.59999832
Produced by FastQC (version 0.11.5)