##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630988.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144090
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276021930737734	31.0	31.0	34.0	28.0	34.0
2	31.31356096883892	31.0	31.0	34.0	28.0	34.0
3	30.763786522312444	31.0	31.0	34.0	26.0	34.0
4	34.89137344715109	37.0	35.0	37.0	32.0	37.0
5	35.10764105767229	35.0	35.0	37.0	32.0	37.0
6	35.31338746616698	37.0	35.0	37.0	33.0	37.0
7	35.295391769033245	37.0	35.0	37.0	32.0	37.0
8	35.33207717398848	37.0	35.0	37.0	32.0	37.0
9	37.115427857589005	39.0	37.0	39.0	33.0	39.0
10	36.957464084946906	39.0	37.0	39.0	33.0	39.0
11	36.975168297591786	39.0	37.0	39.0	33.0	39.0
12	36.872635158581446	39.0	37.0	39.0	33.0	39.0
13	37.024075230758555	39.0	37.0	39.0	33.0	39.0
14	37.87912415851204	40.0	37.0	41.0	33.0	41.0
15	37.885321673953776	39.0	37.0	41.0	33.0	41.0
16	37.91389409396905	40.0	37.0	41.0	33.0	41.0
17	37.95809563467277	40.0	37.0	41.0	33.0	41.0
18	38.07338469012423	40.0	37.0	41.0	33.0	41.0
19	38.05282115344576	40.0	37.0	41.0	33.0	41.0
20	38.01559442015407	40.0	37.0	41.0	33.0	41.0
21	37.96248872232633	40.0	37.0	41.0	33.0	41.0
22	37.78532167395378	39.0	37.0	41.0	33.0	41.0
23	37.83289610660004	39.0	37.0	41.0	33.0	41.0
24	37.73174404885835	39.0	37.0	41.0	32.0	41.0
25	37.64178638351031	39.0	37.0	41.0	32.0	41.0
26	37.402907904781735	39.0	36.0	41.0	32.0	41.0
27	37.31994586716635	39.0	36.0	41.0	31.0	41.0
28	37.27818030397668	39.0	36.0	41.0	31.0	41.0
29	37.23157054618641	39.0	36.0	40.0	31.0	41.0
30	36.992643486709696	39.0	36.0	40.0	31.0	41.0
31	36.81323478381567	39.0	35.0	40.0	31.0	41.0
32	36.53783746269693	38.0	35.0	40.0	30.0	41.0
33	36.135720730099244	38.0	35.0	40.0	30.0	41.0
34	36.1227566104518	38.0	35.0	40.0	30.0	41.0
35	36.07571656603512	38.0	35.0	40.0	29.0	41.0
36	35.98297591782914	38.0	35.0	40.0	28.0	41.0
37	35.913116801998754	38.0	35.0	40.0	28.0	41.0
38	35.77953362481782	38.0	35.0	40.0	27.0	41.0
39	35.52738566173919	38.0	34.0	40.0	26.0	41.0
40	35.031272121590675	38.0	34.0	40.0	26.0	41.0
41	35.05300853633146	38.0	34.0	40.0	26.0	41.0
42	34.82660142966202	38.0	33.0	40.0	24.0	41.0
43	33.83540148518287	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	5.0
11	10.0
12	5.0
13	8.0
14	6.0
15	3.0
16	6.0
17	5.0
18	16.0
19	15.0
20	35.0
21	60.0
22	139.0
23	240.0
24	421.0
25	615.0
26	990.0
27	1506.0
28	2091.0
29	2796.0
30	3538.0
31	4453.0
32	5345.0
33	6839.0
34	8759.0
35	11381.0
36	15509.0
37	22607.0
38	31231.0
39	25447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56506350197793	23.48254563120272	19.038101186758276	24.91428968006107
2	11.9286557012978	21.84259837601499	41.373447151086125	24.855298771601085
3	13.200777291970297	30.852245124574917	36.82559511416476	19.121382469290026
4	8.909709209521827	19.38233048788951	30.23873967659102	41.469220625997636
5	12.470678048441947	37.83885071830106	31.126379346241933	18.56409188701506
6	26.37934624193213	37.21215906724964	15.71170796030259	20.69678673051565
7	21.686445971267958	34.93996807550837	17.367617461308903	26.00596849191478
8	28.81185370254702	28.545353598445416	23.702547019224095	18.94024567978347
9	20.428204594350753	12.210424040530224	15.667985286973419	51.69338607814561
10	15.819279616906101	26.18641127073357	28.593934346588938	29.400374765771392
11	29.298355194670002	20.563536678464846	22.051495593032133	28.086612533833023
12	18.310777985980984	29.4316052467208	21.958498160871677	30.29911860642654
13	33.79762648344784	16.548684849746685	23.749045735304325	25.904642931501144
14	23.49642584495801	24.756749253938512	23.394406273856617	28.352418627246863
15	27.32528280935526	27.4460406690263	19.167881185370256	26.060795336248177
16	28.823651884239016	22.606704143243807	19.030467069192866	29.53917690332431
17	18.40238739676591	34.34450690540634	20.089527378721634	27.16357831910611
18	24.753973211187454	18.250399056145465	24.723436740925813	32.272190991741276
19	23.152890554514542	26.779790408772296	29.244916371712126	20.822402665001043
20	19.662710805746407	28.162259698799364	23.408980498299677	28.76604899715456
21	28.08036643764314	25.331390103407593	25.267541120133252	21.320702338816016
22	25.331390103407593	33.681726698591156	23.367339857033798	17.61954334096745
23	28.900687070580886	20.993823304878894	24.474980914706087	25.63050870983413
24	25.60136026094802	25.449371920327575	25.803317371087513	23.145950447636892
25	26.021930737733364	29.86744395863696	23.238947879797347	20.871677423832327
26	23.232701783607467	30.297036574363247	28.204594350753005	18.265667291276287
27	23.205635366784648	34.531889791102785	25.267541120133252	16.99493372197932
28	18.646679158859047	27.357207300992435	30.489277534874038	23.506836005274483
29	23.741411617738915	22.10979249080436	28.735512526892915	25.413283364563817
30	25.15927545284197	25.343882295787356	31.376223193837184	18.120619057533485
31	22.156291206884585	25.90533694218891	22.637240613505448	29.301131237421057
32	32.1070164480533	21.341522659448955	27.970712748976332	18.58074814352141
33	21.79124158512041	25.55000347005344	32.6538968700118	20.00485807481435
34	18.31771809285863	29.615518078978415	26.182247206606984	25.88451662155597
35	23.186897078215004	20.75577763897564	38.83753209799431	17.219793184815046
36	23.791380387257963	26.39322645568742	23.47629953501284	26.33909362204178
37	20.325491012561596	23.972517176764523	30.456659032549098	25.245332778124784
38	20.638489832743424	19.465611770421265	29.877854118953433	30.018044277881877
39	28.31286001804428	20.81615656881116	26.516760358109515	24.35422305503505
40	17.736137136511903	19.888958289957664	40.83767090013186	21.53723367339857
41	23.579013116802	22.2520646817961	24.645707543896176	29.523214657505726
42	21.603164688736207	19.40315080852245	37.36345339718232	21.630231105559027
43	23.45062113956555	19.12068845860226	33.08557151780138	24.343118884030815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	13.0
2	17.0
3	44.0
4	71.0
5	71.0
6	96.0
7	121.0
8	138.0
9	155.0
10	247.5
11	340.0
12	340.0
13	560.5
14	781.0
15	2053.5
16	3326.0
17	3128.5
18	2931.0
19	2931.0
20	2414.0
21	1897.0
22	1508.5
23	1120.0
24	1104.5
25	1089.0
26	1089.0
27	1212.0
28	1335.0
29	1397.0
30	1459.0
31	1702.0
32	1945.0
33	1945.0
34	2431.0
35	2917.0
36	3300.0
37	3683.0
38	4156.5
39	4630.0
40	4630.0
41	5082.0
42	5534.0
43	6211.0
44	6888.0
45	7976.0
46	9064.0
47	9064.0
48	11037.0
49	13010.0
50	18714.0
51	24418.0
52	17822.5
53	11227.0
54	11227.0
55	16878.0
56	22529.0
57	18120.5
58	13712.0
59	8936.0
60	4160.0
61	4160.0
62	3390.0
63	2620.0
64	1993.5
65	1367.0
66	1143.0
67	919.0
68	919.0
69	690.0
70	461.0
71	342.0
72	223.0
73	131.0
74	39.0
75	39.0
76	33.0
77	27.0
78	29.0
79	31.0
80	20.5
81	10.0
82	10.0
83	7.0
84	4.0
85	8.0
86	12.0
87	10.0
88	8.0
89	8.0
90	4.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	144090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.780415018391285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.49290656042211	18.173363869803595
2	11.513715777855547	5.9365674231383165
3	4.972137723100116	3.845513220903602
4	2.8185317791477105	2.9065167603581092
5	1.9490133792769266	2.5123186897078216
6	1.3029315960912053	2.015407037268374
7	0.9906587342181065	1.7877715316815879
8	0.829138288421676	1.7100423346519535
9	0.6030096643066735	1.3991255465334167
>10	4.046087167200581	19.92921090984801
>50	0.2853527875736937	5.076688180997987
>100	0.1561364309365494	7.355125268929141
>500	0.018844052009583546	3.44021097924908
>1k	0.013460037149702533	9.195641612880838
>5k	0.00807602228982152	14.716496634048164
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7953	5.5194669997917964	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	6969	4.836560483031438	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	6283	4.360469151224929	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	3905	2.71011173572073	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2622	1.8196960233187593	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2617	1.8162259698799361	RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2318	1.6087167742383235	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1788	1.2408911097230897	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	957	0.6641682281907141	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	893	0.6197515441737802	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.5281421333888542	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	685	0.4753973211187452	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	585	0.4059962523422861	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	541	0.375459782080644	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	535	0.37129571795405647	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	481	0.33381914081476854	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	435	0.30189464917759734	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	345	0.2394336872787841	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	343	0.2380456659032549	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	334	0.2317995697133736	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	328	0.22763550558678605	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	318	0.2206953987091401	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	312	0.21653133458255255	No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT	283	0.19640502463737944	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.17836074675550004	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	244	0.16933860781456034	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	234	0.16239850093691444	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	233	0.16170449024914985	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	226	0.1568464154347977	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	220	0.15268235130821015	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	212	0.14713026580609342	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	208	0.14435422305503504	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	203	0.14088416961621208	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	196	0.13602609480185995	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	180	0.12492192379762648	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	177	0.12283989173433271	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	176	0.12214588104656812	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	170	0.11798181691998057	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	169	0.11728780623221598	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	164	0.11381775279339303	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	150	0.10410160316468875	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	150	0.10410160316468875	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	149	0.10340759247692415	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	146	0.10132556041363038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.940106877645916E-4	0.0
10	0.0	0.0	0.0	6.940106877645916E-4	0.0
11	0.0	0.0	0.0	6.940106877645916E-4	0.0
12	0.0	0.0	0.0	6.940106877645916E-4	0.0
13	0.0	0.0	0.0	6.940106877645916E-4	0.0
14	0.0	0.0	0.0	6.940106877645916E-4	0.0
15	0.0	0.0	0.0	6.940106877645916E-4	0.0
16	0.0	0.0	0.0	6.940106877645916E-4	0.0
17	0.0	0.0	0.0	0.0027760427510583664	0.0
18	0.0	0.0	0.0	0.003470053438822958	0.0
19	0.0	0.0	0.0	0.003470053438822958	0.0
20	0.0	0.0	0.0	0.00416406412658755	0.0
21	0.0	0.0	0.0	0.005552085502116733	0.0
22	0.0	0.0	0.0	0.006246096189881324	0.0
23	0.0	0.0	0.0	0.007634117565410508	0.0
24	0.0	0.0	0.0	0.0083281282531751	0.0
25	0.0	0.0	0.0	0.0083281282531751	0.0
26	0.0	0.0	0.0	0.010410160316468874	0.0
27	0.0	0.0	0.0	0.010410160316468874	0.0
28	0.0	0.0	0.0	0.020126309945173157	0.0
29	0.0	0.0	0.0	0.054132833645638145	0.0
30	0.0	0.0	0.0	0.07703518634186966	0.0
31	0.0	0.0	0.0	0.12700395586092025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCGG	20	0.001836597	37.0	23
TGGGGAC	20	0.001836597	37.0	3
GAGCCCG	20	0.001836597	37.0	22
CCGCGTG	20	0.001836597	37.0	37
TGCCGCG	20	0.001836597	37.0	35
ATGCCGC	25	1.2282727E-4	36.999996	34
CTTATAC	715	0.0	34.412586	1
CGTCTTC	335	0.0	32.58209	37
TTATACA	760	0.0	32.375	2
CATGCCG	35	2.37394E-5	31.714285	33
GGTATCA	230	0.0	31.369566	1
GATGTCC	30	3.5840058E-4	30.833334	26
TGTCCTG	30	3.5840058E-4	30.833334	28
TATACAC	815	0.0	30.190184	3
CCGGGGA	25	0.0054805954	29.599998	26
GAAGCAA	25	0.0054805954	29.599998	31
CATATAG	25	0.0054805954	29.599998	3
TGTGGGG	25	0.0054805954	29.599998	1
CCAGCAA	25	0.0054805954	29.599998	30
CCATGCC	25	0.0054805954	29.599998	32
>>END_MODULE