FastQCFastQC Report
Fri 10 Feb 2017
ERR1630987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956377
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT199182.082651506675715No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG142431.4892662621539414No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT125201.3091071826277711RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC72550.7585920614987605No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC49230.5147551645428529RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC34130.3568676369256057RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24830.25962564971763225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.25293372801729863No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19710.20609027611496303No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17810.18622363356709748No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC16450.1720032999538885No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG16200.16938926803969565No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA15690.16405664293474226No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC15400.16102436591427857No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT15200.1589331403829243No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA14310.1496271867683978No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT13610.1423078974086579No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA13160.13760263996311078No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13130.13728895613340764No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12620.13195633102845425No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG11660.12191844847795379No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT11550.12076827443570892No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT10830.11323986252283356No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10790.11282161741656273No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC10760.11250793358685957No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC9960.1041430314614425No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9930.10382934763173937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9750.10194724465352054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22000.033.5522731
CGTCTTC6900.032.44202837
TTATACA24800.029.9133052
TCGGTAT250.00549588729.625
TATACAC25500.029.3098033
GGTATCA5800.025.1982751
CCGTCTT20700.024.3091837
GCCGTCT21050.023.81710236
CGAATTA952.8194336E-1023.36842215
CGGCCTT3000.021.58333424
GATTACG603.725443E-521.5833341
GTATCAA14000.020.7464281
TGCCGTC24400.020.62295235
ATTACGG656.900582E-519.9230772
TTACGGG656.900582E-519.9230773
TATACTG1303.274181E-1119.9230775
ATGCCGT25800.019.57558334
TCTCGCC3550.019.2816912
ATCTCGC3800.018.98684311
TCGCCGA500.00703403818.51
Produced by FastQC (version 0.11.5)