FastQCFastQC Report
Fri 10 Feb 2017
ERR1630986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154148
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT191121.6559401393928683No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG148541.2870099848546286No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT114560.9925936708290445Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC77610.6724440886264154No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC41710.36139212648637786Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC34590.29970159806194696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25900.22440796154392675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23210.201100725383573No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG20730.17961301323573753No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13850.12000194082561336No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA13600.11783584081071058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC24400.032.147541
TATACAC26600.029.7669163
CGTCTTC5700.029.53508837
TTATACA27050.029.0665422
CCGTCTT17350.024.8443837
GCCGTCT17600.024.5965936
GTATCAA14750.023.2033881
GGTATCA5900.021.9491521
CGAACTA1151.3460522E-1020.91304429
CGAGTAG555.143112E-420.18181818
GCGGTAA555.143112E-420.18181823
TGCCGTC21600.020.12731635
ATGCCGT22500.019.32222234
CGATTGG500.007034899718.49999837
ACGATCA500.007034899718.49999816
CCGTTAA500.007034899718.49999816
ATACACA44050.018.3950044
TACACAT44300.018.2076765
TATGCCG25000.017.61233
TTAACGG852.7231308E-517.41176435
Produced by FastQC (version 0.11.5)