##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630986.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1154148
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46532420452143	31.0	31.0	34.0	28.0	34.0
2	31.463048933065778	31.0	31.0	34.0	28.0	34.0
3	30.939300678942388	31.0	31.0	34.0	27.0	34.0
4	35.09422101844824	37.0	35.0	37.0	32.0	37.0
5	35.280606993210576	37.0	35.0	37.0	33.0	37.0
6	35.46625995972787	37.0	35.0	37.0	33.0	37.0
7	35.46922405098826	37.0	35.0	37.0	33.0	37.0
8	35.491393651420786	37.0	35.0	37.0	33.0	37.0
9	37.26618249999134	39.0	37.0	39.0	34.0	39.0
10	37.093215948041326	39.0	37.0	39.0	33.0	39.0
11	37.15915029961496	39.0	37.0	39.0	33.0	39.0
12	37.09241449103581	39.0	37.0	39.0	33.0	39.0
13	37.16522057829672	39.0	37.0	39.0	33.0	39.0
14	38.305035402738646	40.0	38.0	41.0	34.0	41.0
15	38.318812665273434	40.0	38.0	41.0	34.0	41.0
16	38.306259682467065	40.0	38.0	41.0	33.0	41.0
17	38.27906299711995	40.0	38.0	41.0	33.0	41.0
18	38.31351438463698	40.0	38.0	41.0	33.0	41.0
19	38.32969688462831	40.0	38.0	41.0	34.0	41.0
20	38.33585640663069	40.0	38.0	41.0	34.0	41.0
21	38.28832610722368	40.0	38.0	41.0	34.0	41.0
22	38.229820612261165	40.0	38.0	41.0	34.0	41.0
23	38.227278477283676	40.0	38.0	41.0	34.0	41.0
24	38.19218852348226	40.0	38.0	41.0	33.0	41.0
25	38.17942672863445	40.0	38.0	41.0	33.0	41.0
26	38.04655295508029	40.0	37.0	41.0	33.0	41.0
27	37.99512540852646	40.0	37.0	41.0	33.0	41.0
28	37.971140616281446	40.0	37.0	41.0	33.0	41.0
29	37.864730519829344	40.0	37.0	41.0	33.0	41.0
30	37.81985845836063	40.0	37.0	41.0	33.0	41.0
31	37.78447131563716	40.0	37.0	41.0	33.0	41.0
32	37.697809986240934	40.0	37.0	41.0	33.0	41.0
33	37.69047643803048	40.0	37.0	41.0	33.0	41.0
34	37.63437271476448	40.0	37.0	41.0	32.0	41.0
35	37.644116699071525	40.0	37.0	41.0	32.0	41.0
36	37.61716694912611	40.0	37.0	41.0	32.0	41.0
37	37.556264881107104	40.0	37.0	41.0	32.0	41.0
38	37.52506091073242	40.0	37.0	41.0	32.0	41.0
39	37.453505096400114	40.0	37.0	41.0	32.0	41.0
40	37.359955568956494	39.0	36.0	41.0	31.0	41.0
41	37.307080201152715	39.0	36.0	41.0	31.0	41.0
42	37.25943033302488	39.0	36.0	41.0	31.0	41.0
43	36.60982300363558	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	18.0
10	12.0
11	13.0
12	20.0
13	14.0
14	6.0
15	10.0
16	12.0
17	12.0
18	24.0
19	35.0
20	110.0
21	200.0
22	392.0
23	775.0
24	1386.0
25	2411.0
26	3963.0
27	6377.0
28	9410.0
29	13474.0
30	18641.0
31	25005.0
32	33090.0
33	43141.0
34	56862.0
35	76735.0
36	106479.0
37	159130.0
38	279179.0
39	317207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.2016864388276	22.233370416965588	14.849568686165032	24.715374458041776
2	13.717824750378634	21.804309326013648	41.06171825450462	23.416147669103097
3	14.601160336455985	28.29975011870228	34.007683589972864	23.091405954868875
4	11.69087500043322	19.23202223631631	38.16113704654862	30.915965716701844
5	12.67489091520325	37.99850625742972	35.95786675539012	13.368736071976905
6	27.959239196359565	40.15680831227884	17.714885785878415	14.16906670548318
7	24.670839441735374	33.19920842041056	22.962566325982454	19.167385811871615
8	24.531169312774445	35.72557418979195	22.00385045938649	17.739406038047115
9	24.026814585304486	15.008820359260683	19.976900709441075	40.98746434599376
10	14.194193465656053	29.025220335693515	33.91809369335648	22.86249250529395
11	31.852240787143415	22.956587889941325	25.479661187300067	19.71151013561519
12	20.62179200587793	25.969979586673457	29.932816241937775	23.475412165510836
13	28.609762352835165	20.95034605613838	27.0384734020247	23.401418189001756
14	21.295709042514478	22.051244727712564	26.759653008106415	29.893393221666546
15	23.415887737101308	28.92064102697401	22.98587356214281	24.67759767378187
16	24.450677036220657	27.766976159036794	23.782825079625837	23.99952172511671
17	21.025119828652823	30.271854216270356	25.47143000724344	23.23159594783338
18	23.66507588281572	26.23545680449994	26.087902071484763	24.01156524119957
19	23.796688119721214	27.28012351968725	25.674870120643106	23.24831823994843
20	23.823894335908395	26.13416996780309	25.752329857176033	24.28960583911249
21	24.787808842540123	26.353379289311253	25.648183768459504	23.21062809968912
22	22.065454343810327	30.434571649389852	25.996752582857656	21.50322142394216
23	24.212579322582545	25.742365797107475	26.038601635145582	24.0064532451644
24	23.31997282844141	27.56353604563713	25.452368327112296	23.664122798809164
25	22.996184198213747	27.561803165625204	26.13590284781501	23.306109788346035
26	24.18459331039	26.452759957994985	26.55205398267813	22.810592748936877
27	22.99107220217858	29.17190862870273	25.894685950155438	21.942333218963253
28	23.34050745658269	25.70043010081896	27.23862104340171	23.720441399196638
29	21.97508465118858	27.27925707968129	25.908808922252607	24.836849346877525
30	24.816834582739823	25.727896249007927	27.2811632476944	22.17410592055785
31	24.399730363870145	25.496296835414523	26.350693325292767	23.753279475422563
32	22.15114526039988	27.8070056873122	26.8157116764921	23.226137375795826
33	22.958927277957418	25.92189216634262	29.256993037288115	21.86218751841185
34	21.5157848040286	27.5909155498255	26.604733535040566	24.288566111105332
35	21.588998984532314	25.907335974242475	29.48937224688688	23.014292794338335
36	22.95468172192821	25.941127134474957	27.496560233176336	23.6076309104205
37	22.063894751799594	27.06108748618028	27.802586843281794	23.072430918738323
38	22.095086592014194	25.593164828080972	28.20964035808233	24.102108221822505
39	23.554691426056277	25.904996586226375	26.989952761690876	23.550359226026472
40	21.59402433656689	25.467011163213037	30.9146660566929	22.024298443527172
41	22.549707663141987	26.210070112325283	26.584892058904057	24.655330165628673
42	22.839705133137173	25.571330539930752	29.037697071779355	22.55126725515272
43	23.121211491073936	25.070181640482847	29.005118927555216	22.803487940887997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	12.5
2	19.0
3	86.0
4	153.0
5	153.0
6	188.5
7	224.0
8	240.0
9	256.0
10	379.5
11	503.0
12	503.0
13	775.0
14	1047.0
15	2569.5
16	4092.0
17	4919.5
18	5747.0
19	5747.0
20	6313.5
21	6880.0
22	8570.0
23	10260.0
24	13755.0
25	17250.0
26	17250.0
27	21837.0
28	26424.0
29	31433.5
30	36443.0
31	42580.0
32	48717.0
33	48717.0
34	55550.0
35	62383.0
36	66502.0
37	70621.0
38	75940.5
39	81260.0
40	81260.0
41	83585.5
42	85911.0
43	87113.5
44	88316.0
45	88928.5
46	89541.0
47	89541.0
48	86531.5
49	83522.0
50	80238.5
51	76955.0
52	74292.5
53	71630.0
54	71630.0
55	83770.5
56	95911.0
57	72202.0
58	48493.0
59	52863.5
60	57234.0
61	57234.0
62	47238.0
63	37242.0
64	27644.0
65	18046.0
66	14882.5
67	11719.0
68	11719.0
69	9620.5
70	7522.0
71	6031.5
72	4541.0
73	3522.5
74	2504.0
75	2504.0
76	1923.0
77	1342.0
78	1027.0
79	712.0
80	540.5
81	369.0
82	369.0
83	278.0
84	187.0
85	147.0
86	107.0
87	75.0
88	43.0
89	43.0
90	28.5
91	14.0
92	8.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1154148.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.13843203890233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58182518761029	41.2257654518583
2	12.975785496803965	13.790257915465839
3	3.9749956025033852	6.336751010356852
4	1.727286226555546	3.671411270462157
5	0.9192731142209815	2.442436595261086
6	0.5843284610571434	1.863017892976884
7	0.37231372474949204	1.3848917291826028
8	0.2732622514452281	1.1616582061775753
9	0.2043817124492769	0.977447136328234
>10	1.2292650417995117	12.242841261061189
>50	0.10623222531348409	3.867674341799977
>100	0.047299609143860305	4.168821359497851
>500	0.0017941221664083315	0.6585175466110267
>1k	0.0013048161210242413	1.5991454662437554
>5k	1.6310201512803016E-4	0.6726447327254775
>10k+	4.893060453840904E-4	3.936718083991321
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	19112	1.6559401393928683	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	14854	1.2870099848546286	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	11456	0.9925936708290445	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	7761	0.6724440886264154	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	4171	0.36139212648637786	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3459	0.29970159806194696	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2590	0.22440796154392675	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.201100725383573	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	2073	0.17961301323573753	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1385	0.12000194082561336	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1360	0.11783584081071058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.664400059611072E-5	0.0
4	0.0	0.0	0.0	8.664400059611072E-5	0.0
5	0.0	0.0	0.0	1.7328800119222144E-4	0.0
6	0.0	0.0	0.0	1.7328800119222144E-4	0.0
7	0.0	0.0	0.0	1.7328800119222144E-4	0.0
8	0.0	0.0	0.0	1.7328800119222144E-4	0.0
9	0.0	0.0	0.0	2.5993200178833217E-4	0.0
10	0.0	0.0	0.0	4.3322000298055363E-4	0.0
11	0.0	0.0	0.0	5.198640035766643E-4	0.0
12	0.0	0.0	0.0	5.198640035766643E-4	0.0
13	0.0	0.0	0.0	6.06508004172775E-4	0.0
14	0.0	0.0	0.0	6.06508004172775E-4	0.0
15	0.0	0.0	0.0	6.06508004172775E-4	0.0
16	0.0	0.0	0.0	6.931520047688857E-4	8.664400059611072E-5
17	0.0	0.0	0.0	7.797960053649966E-4	8.664400059611072E-5
18	0.0	0.0	0.0	7.797960053649966E-4	8.664400059611072E-5
19	0.0	0.0	0.0	7.797960053649966E-4	8.664400059611072E-5
20	0.0	0.0	0.0	9.53084006557218E-4	8.664400059611072E-5
21	0.0	0.0	0.0	0.0012996600089416608	8.664400059611072E-5
22	0.0	0.0	0.0	0.0015595920107299931	8.664400059611072E-5
23	0.0	0.0	0.0	0.001906168013114436	8.664400059611072E-5
24	0.0	0.0	0.0	0.0022527440154988788	8.664400059611072E-5
25	0.0	0.0	0.0	0.002512676017287211	8.664400059611072E-5
26	0.0	0.0	0.0	0.0026859640184794323	8.664400059611072E-5
27	0.0	0.0	0.0	0.002945896020267765	8.664400059611072E-5
28	0.0	0.0	0.0	0.007364740050669411	8.664400059611072E-5
29	0.0	0.0	0.0	0.01689558011624159	8.664400059611072E-5
30	0.0	0.0	0.0	0.031884992219368746	8.664400059611072E-5
31	0.0	0.0	0.0	0.07052821648523414	8.664400059611072E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2440	0.0	32.14754	1
TATACAC	2660	0.0	29.766916	3
CGTCTTC	570	0.0	29.535088	37
TTATACA	2705	0.0	29.066542	2
CCGTCTT	1735	0.0	24.84438	37
GCCGTCT	1760	0.0	24.59659	36
GTATCAA	1475	0.0	23.203388	1
GGTATCA	590	0.0	21.949152	1
CGAACTA	115	1.3460522E-10	20.913044	29
CGAGTAG	55	5.143112E-4	20.181818	18
GCGGTAA	55	5.143112E-4	20.181818	23
TGCCGTC	2160	0.0	20.127316	35
ATGCCGT	2250	0.0	19.322222	34
CGATTGG	50	0.0070348997	18.499998	37
ACGATCA	50	0.0070348997	18.499998	16
CCGTTAA	50	0.0070348997	18.499998	16
ATACACA	4405	0.0	18.395004	4
TACACAT	4430	0.0	18.207676	5
TATGCCG	2500	0.0	17.612	33
TTAACGG	85	2.7231308E-5	17.411764	35
>>END_MODULE