FastQCFastQC Report
Fri 10 Feb 2017
ERR1630985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203724
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG130476.404252812628851No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT113645.578135123991283No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT99594.888476566334845RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC68393.3569927941725077No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC37541.8426891284286584RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC33871.6625434411262296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29441.4450923798865132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26641.307651528538611No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16560.8128644636861637No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG15630.7672144666313248No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA11340.5566354479590033No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11150.5473091044746814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10960.5379827609903595No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10750.5276746971392668No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT10510.5158940527380181No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10100.4957687852192182No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8830.4334295419292769No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8210.4029962105593842RNA PCR Primer, Index 26 (95% over 21bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7210.3539101922208478No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7180.3524376116706917No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7140.3504741709371503No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6360.3121870766330918No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5850.28715320728043825No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5350.26261019811117003No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5120.2513204138933066No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4930.24199407040898469No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4810.23610374820836033No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4370.2145059001394043No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4230.2076338575720092No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4220.20714299738862382No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4210.20665213720523845No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3930.19290805207044825No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3920.19241719188706288No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3780.18554514931966778No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3730.18309084840274095No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3610.1772005262021166No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3600.17670966601873123No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3550.1742553651018044No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3490.17131020400149222No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3440.1688559030845654No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3430.16836504290118004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.16640160216763858No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3360.16492902161748246No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3340.16394730125071175RNA PCR Primer, Index 26 (95% over 23bp)
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3250.15952955960024348No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2930.1438220337319118No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2930.1438220337319118No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2890.14185859299837036No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2810.13793171153128742No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2810.13793171153128742No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2800.13744085134790207No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2800.13744085134790207No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2730.1340048300642045No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2690.13204138933066306No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2660.13056880878050695No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2610.12811450786358014No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2590.12713278749680942No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2590.12713278749680942No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2530.12418762639649723No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2470.12124246529618503No Hit
CTGATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT2450.1202607449294143No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2360.11584300327894602No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2350.11535214309556065No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2340.1148612829121753No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2330.11437042272878994No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2320.11387956254540457No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2240.10995268107832165No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10897096071155092No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2210.10848010052816556No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2180.10700751997800946No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2170.10651665979462409No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA2140.10504407924446801No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2120.10406235887769728No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2080.10209891814415581No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2050.10062633759399973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAGA355.601032E-737.01
CTCAGAG355.601032E-737.02
GGGATCA200.001838383437.01
ATTACGG251.2300632E-436.9999962
GATTACG251.2300632E-436.9999961
CGTCTTC4700.035.03191437
CTTATAC12600.034.9444431
TTATACA13400.032.7201462
TCAGAGT401.5915248E-632.3753
CAGACAC352.3787434E-531.71428518
GGTATCA4000.031.4499991
TCCCATG303.5891807E-430.8333342
TTACGGG303.5891807E-430.8333343
TATACAC14400.030.576393
GATACAC250.005485865329.5999983
GGATCAA250.005485865329.5999982
TGATACA250.005485865329.5999982
GATCAAC250.005485865329.5999983
TACTATA405.91653E-527.7500022
CTCCCAT358.844263E-426.428571
Produced by FastQC (version 0.11.5)