##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630985.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 203724 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.767764230036715 31.0 30.0 33.0 27.0 34.0 2 30.7660511279967 31.0 30.0 34.0 27.0 34.0 3 30.167496220376588 31.0 30.0 34.0 26.0 34.0 4 34.38926194262826 35.0 35.0 37.0 30.0 37.0 5 34.55952170583731 35.0 35.0 37.0 32.0 37.0 6 34.793092615499404 35.0 35.0 37.0 32.0 37.0 7 34.79543401857415 35.0 35.0 37.0 32.0 37.0 8 34.833853645127725 35.0 35.0 37.0 32.0 37.0 9 36.547745970037894 38.0 35.0 39.0 32.0 39.0 10 36.36176395515501 38.0 35.0 39.0 32.0 39.0 11 36.38389193222203 38.0 35.0 39.0 32.0 39.0 12 36.240865091987196 38.0 35.0 39.0 32.0 39.0 13 36.383003475290096 38.0 35.0 39.0 32.0 39.0 14 37.05214898588286 38.0 36.0 40.0 31.0 41.0 15 37.0308407453221 38.0 36.0 40.0 31.0 41.0 16 37.03424240639296 38.0 36.0 40.0 31.0 41.0 17 36.976311087549824 38.0 36.0 40.0 31.0 41.0 18 37.21763267950757 39.0 36.0 40.0 32.0 41.0 19 37.154071194360995 39.0 36.0 40.0 31.0 41.0 20 37.22202587814887 39.0 36.0 40.0 32.0 41.0 21 37.19922542263062 39.0 36.0 40.0 32.0 41.0 22 36.8182344740924 38.0 36.0 40.0 31.0 41.0 23 37.00968958002003 39.0 36.0 40.0 31.0 41.0 24 36.6826785258487 38.0 35.0 40.0 30.0 41.0 25 36.63905087274941 38.0 35.0 40.0 30.0 41.0 26 36.50186526869687 38.0 35.0 40.0 30.0 41.0 27 36.27322259527596 38.0 35.0 40.0 30.0 41.0 28 36.434087294575015 38.0 35.0 40.0 30.0 41.0 29 36.3062574856178 38.0 35.0 40.0 30.0 41.0 30 36.12006440085606 38.0 35.0 40.0 30.0 41.0 31 35.97502503386935 38.0 35.0 40.0 30.0 41.0 32 35.71989063635114 38.0 34.0 40.0 29.0 41.0 33 35.512350042213974 38.0 34.0 40.0 29.0 41.0 34 35.37226836307946 38.0 34.0 40.0 28.0 41.0 35 35.36747756768962 38.0 34.0 40.0 27.0 41.0 36 35.234159941882154 38.0 34.0 40.0 27.0 41.0 37 35.18443580530522 38.0 34.0 40.0 27.0 41.0 38 35.04699495395732 38.0 33.0 40.0 26.0 41.0 39 34.81450393669867 37.0 33.0 40.0 26.0 41.0 40 34.390032593116175 37.0 33.0 40.0 24.0 41.0 41 34.330545247491706 37.0 33.0 40.0 24.0 41.0 42 34.07433586617188 37.0 33.0 40.0 23.0 41.0 43 32.915287349551356 36.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 2.0 9 5.0 10 11.0 11 8.0 12 14.0 13 11.0 14 10.0 15 10.0 16 11.0 17 14.0 18 21.0 19 34.0 20 77.0 21 139.0 22 280.0 23 506.0 24 817.0 25 1165.0 26 1841.0 27 2661.0 28 3688.0 29 4961.0 30 6421.0 31 8031.0 32 9921.0 33 12472.0 34 16133.0 35 20504.0 36 26795.0 37 34577.0 38 37707.0 39 14876.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.844927450864894 24.149339302193162 17.607154778033024 25.398578468908916 2 12.62983251850543 21.5320728043824 42.36270640670711 23.475388270405055 3 14.687518407256878 31.95008933655338 35.305609550175724 18.05678270601402 4 9.41175315623098 20.725589523080245 27.95350572342974 41.90915159725904 5 13.189903987748131 37.96459916357425 29.616049164555967 19.229447684121656 6 25.452573089081305 36.556812157625025 15.6996721053975 22.290942647896173 7 21.34260077359565 34.40340853311343 15.638805442657713 28.615185250633207 8 27.264828886140073 28.515540633405976 25.40790481239324 18.81172566806071 9 20.05507451257584 11.12927293789637 14.331153914119103 54.484498635408684 10 15.687400600812865 26.397478942098136 27.74047240384049 30.174648053248514 11 30.451984056861242 19.206377255502545 21.912489446506058 28.42914924113016 12 18.876519212267578 27.255993402839135 20.84585026800966 33.02163711688363 13 36.576446564960435 15.751703284836346 23.442991498301623 24.228858651901593 14 22.67381359093676 25.6003220042803 22.445563605662564 29.28030079912038 15 29.28422768058746 24.41587638177142 18.170662268559422 28.1292336690817 16 28.65445406530404 21.675403977930927 17.49671123677132 32.17343071999372 17 18.814179968977637 33.66171879601814 18.226620329465355 29.297480905538865 18 26.366063890361467 16.07321670495376 23.326166774655906 34.234552630028865 19 24.55331723311932 24.997054838899686 25.955704777051302 24.49392315092969 20 20.17239009640494 18.982054151695433 21.970901808328914 38.87465394357071 21 29.47222713082406 17.43928059531523 25.08344623117551 28.0050460426852 22 19.721780448057174 33.94641770238165 26.772986982387938 19.558814867173236 23 30.277237831576052 19.163672419548018 22.76216842394612 27.79692132492981 24 25.668060709587483 28.770297068582984 26.939388584555573 18.622253637273957 25 24.99116451669906 24.63038228191082 21.724489996269465 28.653963205120654 26 24.37562584673382 21.556124953368283 28.6490546032868 25.4191945966111 27 20.7103728573953 28.18421000962086 27.949087981779268 23.15632915120457 28 19.309948754196853 18.875537491900808 27.491115430680722 34.32339832322162 29 18.621762777090574 22.555516286740882 34.58109991949893 24.241621016669612 30 26.545718717480515 18.08819775775068 29.65286367830987 25.713219846458934 31 22.506430268402347 23.038031847008696 21.089807779152185 33.36573010543677 32 27.970194969664842 20.828670161591173 32.289273723272665 18.911861145471324 33 23.610374820836032 19.429227778759497 31.00420176316978 25.956195637234693 34 17.531562309791678 35.18633052561309 24.18811725668061 23.09398990791463 35 24.426675305805894 19.313875635663937 38.079951306669805 18.17949775186036 36 22.90353615676111 19.80522667923269 22.61343778838036 34.677799375625845 37 21.88451041605309 24.144921560542695 29.744654532602933 24.22591349080128 38 20.653433076122596 17.84816712807524 30.157467946830025 31.340931848972136 39 29.93559914393984 19.191651450000982 24.943551078910684 25.929198327148494 40 18.07788969389959 18.95652942215939 41.20869411556812 21.756886768372897 41 24.656397871630244 21.833460956981014 23.279535057234295 30.230606114154448 42 23.55687106084703 17.77404724038405 36.265241208694114 22.40384049007481 43 25.526692976772498 18.114213347470105 31.918183424633327 24.44091025112407 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 2.0 2 4.0 3 27.0 4 50.0 5 50.0 6 96.0 7 142.0 8 168.5 9 195.0 10 309.0 11 423.0 12 423.0 13 723.5 14 1024.0 15 2664.5 16 4305.0 17 4318.0 18 4331.0 19 4331.0 20 3609.0 21 2887.0 22 2221.5 23 1556.0 24 1478.0 25 1400.0 26 1400.0 27 1364.0 28 1328.0 29 1264.5 30 1201.0 31 1241.5 32 1282.0 33 1282.0 34 1577.0 35 1872.0 36 1906.0 37 1940.0 38 2133.0 39 2326.0 40 2326.0 41 2947.5 42 3569.0 43 4992.5 44 6416.0 45 8893.0 46 11370.0 47 11370.0 48 14491.5 49 17613.0 50 18290.5 51 18968.0 52 31339.0 53 43710.0 54 43710.0 55 30602.0 56 17494.0 57 24153.0 58 30812.0 59 23516.5 60 16221.0 61 16221.0 62 10685.5 63 5150.0 64 3980.5 65 2811.0 66 2315.0 67 1819.0 68 1819.0 69 1422.5 70 1026.0 71 693.0 72 360.0 73 212.5 74 65.0 75 65.0 76 48.5 77 32.0 78 22.0 79 12.0 80 8.0 81 4.0 82 4.0 83 3.5 84 3.0 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 203724.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.48997663505527 #Duplication Level Percentage of deduplicated Percentage of total 1 74.26394844644612 15.216665684946301 2 10.387370337541624 4.256739510317882 3 3.983901492465803 2.4489014549095836 4 2.3644682940852357 1.9379160040054193 5 1.4469491890855952 1.482397753823801 6 1.0828162805739885 1.3312128173411086 7 0.7881560980284119 1.1304510023364944 8 0.5581774189684499 0.9149633818303196 9 0.5294300840859545 0.97632090475349 >10 3.7060105886016816 15.314346861439986 >50 0.4815178592817958 6.9348726708684305 >100 0.3473636298301512 13.801515776246296 >500 0.021560501161871453 3.006518623235358 >1k 0.02874733488249527 11.019320256818048 >5k 0.004791222480415878 8.245469360507354 >10k+ 0.004791222480415878 11.982387936620134 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG 13047 6.404252812628851 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT 11364 5.578135123991283 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 9959 4.888476566334845 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC 6839 3.3569927941725077 No Hit CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC 3754 1.8426891284286584 RNA PCR Primer, Index 26 (100% over 23bp) CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 3387 1.6625434411262296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 1.4450923798865132 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2664 1.307651528538611 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 1656 0.8128644636861637 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG 1563 0.7672144666313248 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA 1134 0.5566354479590033 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 1115 0.5473091044746814 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.5379827609903595 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 1075 0.5276746971392668 No Hit TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT 1051 0.5158940527380181 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 1010 0.4957687852192182 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 883 0.4334295419292769 No Hit ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 821 0.4029962105593842 RNA PCR Primer, Index 26 (95% over 21bp) CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 721 0.3539101922208478 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 718 0.3524376116706917 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 714 0.3504741709371503 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 636 0.3121870766330918 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 585 0.28715320728043825 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 535 0.26261019811117003 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 512 0.2513204138933066 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 493 0.24199407040898469 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 481 0.23610374820836033 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 437 0.2145059001394043 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 423 0.2076338575720092 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 422 0.20714299738862382 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 421 0.20665213720523845 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 393 0.19290805207044825 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 392 0.19241719188706288 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 378 0.18554514931966778 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 373 0.18309084840274095 No Hit AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT 361 0.1772005262021166 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 360 0.17670966601873123 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 355 0.1742553651018044 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 349 0.17131020400149222 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 344 0.1688559030845654 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 343 0.16836504290118004 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.16640160216763858 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 336 0.16492902161748246 No Hit ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 334 0.16394730125071175 RNA PCR Primer, Index 26 (95% over 23bp) CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG 325 0.15952955960024348 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 293 0.1438220337319118 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 293 0.1438220337319118 No Hit GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG 289 0.14185859299837036 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 281 0.13793171153128742 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG 281 0.13793171153128742 No Hit ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC 280 0.13744085134790207 No Hit CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT 280 0.13744085134790207 No Hit GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG 273 0.1340048300642045 No Hit CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC 269 0.13204138933066306 No Hit GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA 266 0.13056880878050695 No Hit ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA 261 0.12811450786358014 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 259 0.12713278749680942 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA 259 0.12713278749680942 No Hit TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA 253 0.12418762639649723 No Hit TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA 247 0.12124246529618503 No Hit CTGATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT 245 0.1202607449294143 No Hit CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA 236 0.11584300327894602 No Hit CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG 235 0.11535214309556065 No Hit AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA 234 0.1148612829121753 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 233 0.11437042272878994 No Hit GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG 232 0.11387956254540457 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 224 0.10995268107832165 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 222 0.10897096071155092 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT 221 0.10848010052816556 No Hit CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT 218 0.10700751997800946 No Hit GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 217 0.10651665979462409 No Hit CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA 214 0.10504407924446801 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 212 0.10406235887769728 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 208 0.10209891814415581 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC 205 0.10062633759399973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 4.908601833853645E-4 0.0 18 0.0 0.0 0.0 9.81720366770729E-4 0.0 19 0.0 0.0 0.0 9.81720366770729E-4 0.0 20 0.0 0.0 0.0 9.81720366770729E-4 0.0 21 0.0 0.0 0.0 0.0014725805501560935 0.0 22 0.0 0.0 0.0 0.0014725805501560935 0.0 23 0.0 0.0 0.0 0.0024543009169268226 0.0 24 0.0 0.0 0.0 0.002945161100312187 0.0 25 0.0 0.0 0.0 0.002945161100312187 0.0 26 0.0 0.0 0.0 0.0034360212836975516 0.0 27 0.0 0.0 0.0 0.004417741650468281 0.0 28 0.0 0.0 0.0 0.01619838605171703 0.0 29 0.0 0.0 0.0 0.04368655632129744 0.0 30 0.0 0.0 0.0 0.07706504879150222 0.0 31 0.0 0.0 0.0 0.13400483006420452 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCAGA 35 5.601032E-7 37.0 1 CTCAGAG 35 5.601032E-7 37.0 2 GGGATCA 20 0.0018383834 37.0 1 ATTACGG 25 1.2300632E-4 36.999996 2 GATTACG 25 1.2300632E-4 36.999996 1 CGTCTTC 470 0.0 35.031914 37 CTTATAC 1260 0.0 34.944443 1 TTATACA 1340 0.0 32.720146 2 TCAGAGT 40 1.5915248E-6 32.375 3 CAGACAC 35 2.3787434E-5 31.714285 18 GGTATCA 400 0.0 31.449999 1 TCCCATG 30 3.5891807E-4 30.833334 2 TTACGGG 30 3.5891807E-4 30.833334 3 TATACAC 1440 0.0 30.57639 3 GATACAC 25 0.0054858653 29.599998 3 GGATCAA 25 0.0054858653 29.599998 2 TGATACA 25 0.0054858653 29.599998 2 GATCAAC 25 0.0054858653 29.599998 3 TACTATA 40 5.91653E-5 27.750002 2 CTCCCAT 35 8.844263E-4 26.42857 1 >>END_MODULE