FastQCFastQC Report
Fri 10 Feb 2017
ERR1630984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878474
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT168831.9218553992491525No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG151261.7218494798935426No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT105161.1970758383287383TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC77310.8800488119170289No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC37720.4293809492369723RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC33260.3786110915064077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28140.32032820550181335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25160.28640574450695183No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG19080.21719481737649607No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA13610.15492774971143142No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13070.14878072657813435No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC12550.14286137096829274No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11580.1318194960807036No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10850.12350963147457977No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT10800.12294046266594115No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC10230.11645193824746095No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT10040.1142890967746342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9890.11258159034871834No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC9720.11064641639934704No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9300.10586539840678266No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9100.10358872317222821No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8880.1010843804142183No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT8860.10085671289076284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG251.2333888E-437.05
CTTATAC20800.033.175481
GATTACG950.031.1578961
TTATACA22100.031.0565592
TATACAC22600.030.6969033
GGTATCA5850.030.0427361
CGCCGTG250.005495646529.637
CGTCTTC5500.028.9272737
TTACGGG1250.025.163
CCGTCTT16650.024.66666637
GCCGTCT17550.023.40170936
CGAGTAA400.001930826323.12500221
TGTTACG400.001930826323.12500217
GTATCAA16350.023.0825671
ATTACGG1300.022.769232
ACGGTAC1450.021.6896553
CGACACG705.099664E-621.14285721
TCGTACT705.099664E-621.14285711
CACGGTA1550.020.2903212
GCGGGTA555.141766E-420.18181822
Produced by FastQC (version 0.11.5)