##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630984.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878474
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.328266972044705	31.0	31.0	34.0	28.0	34.0
2	31.324024387745112	31.0	31.0	34.0	28.0	34.0
3	30.771270407547632	31.0	31.0	34.0	26.0	34.0
4	34.9628139250564	37.0	35.0	37.0	32.0	37.0
5	35.147152903785425	37.0	35.0	37.0	32.0	37.0
6	35.3549439141056	37.0	35.0	37.0	33.0	37.0
7	35.36460498546343	37.0	35.0	37.0	33.0	37.0
8	35.39370317163627	37.0	35.0	37.0	33.0	37.0
9	37.15267384122922	39.0	37.0	39.0	33.0	39.0
10	36.96057595330084	39.0	37.0	39.0	33.0	39.0
11	37.028623499386434	39.0	37.0	39.0	33.0	39.0
12	36.96054180317232	39.0	37.0	39.0	33.0	39.0
13	37.04297452172745	39.0	37.0	39.0	33.0	39.0
14	38.123219355382176	40.0	37.0	41.0	33.0	41.0
15	38.13683842663528	40.0	37.0	41.0	33.0	41.0
16	38.12766114876479	40.0	37.0	41.0	33.0	41.0
17	38.09074827484934	40.0	37.0	41.0	33.0	41.0
18	38.14839369178826	40.0	37.0	41.0	33.0	41.0
19	38.16596734792379	40.0	38.0	41.0	33.0	41.0
20	38.1537859970813	40.0	38.0	41.0	33.0	41.0
21	38.105235897704425	40.0	37.0	41.0	33.0	41.0
22	38.016015271937476	40.0	37.0	41.0	33.0	41.0
23	38.021071767633416	40.0	37.0	41.0	33.0	41.0
24	37.94088271252194	40.0	37.0	41.0	33.0	41.0
25	37.88983510041276	40.0	37.0	41.0	33.0	41.0
26	37.783706746016385	40.0	37.0	41.0	33.0	41.0
27	37.700882439320914	40.0	37.0	41.0	32.0	41.0
28	37.67514348745666	40.0	37.0	41.0	32.0	41.0
29	37.57471137449714	39.0	37.0	41.0	32.0	41.0
30	37.46764616824175	39.0	37.0	41.0	32.0	41.0
31	37.456624783431266	39.0	37.0	41.0	32.0	41.0
32	37.28164863160435	39.0	36.0	40.0	31.0	41.0
33	37.276965510646875	39.0	36.0	40.0	31.0	41.0
34	37.252432058319314	39.0	36.0	40.0	31.0	41.0
35	37.16861967457204	39.0	36.0	40.0	31.0	41.0
36	37.173677308605605	39.0	36.0	40.0	31.0	41.0
37	37.16432700341729	39.0	36.0	40.0	31.0	41.0
38	37.117186166010605	39.0	36.0	40.0	31.0	41.0
39	37.02087142021278	39.0	36.0	40.0	31.0	41.0
40	36.928569314515855	39.0	36.0	40.0	31.0	41.0
41	36.88601825438203	39.0	36.0	40.0	31.0	41.0
42	36.837965608543904	39.0	36.0	40.0	30.0	41.0
43	36.087745340214965	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	12.0
11	19.0
12	12.0
13	13.0
14	17.0
15	13.0
16	16.0
17	10.0
18	21.0
19	38.0
20	98.0
21	229.0
22	376.0
23	842.0
24	1415.0
25	2380.0
26	3882.0
27	5617.0
28	8369.0
29	11721.0
30	16058.0
31	20903.0
32	27711.0
33	35522.0
34	47167.0
35	63636.0
36	87799.0
37	131524.0
38	222112.0
39	190938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.56013268463267	22.80534199077036	15.5307954475602	24.10372987703677
2	13.730173004551075	22.45439250336379	41.366164507999095	22.44926998408604
3	14.790534495044817	29.085322957765396	34.79055726179716	21.333585285392623
4	11.711445074071628	19.796488000783178	37.26143289385912	31.23063403128607
5	12.081290965925001	39.00365861710193	35.72490477805832	13.190145638914755
6	26.844846859440345	40.164990654248165	17.962512265587826	15.027650220723665
7	23.548107286043756	33.89536855957034	22.661911450993426	19.894612703392472
8	24.083922802496147	35.60219198291583	22.649617404726833	17.664267809861194
9	23.52875554655004	15.454868328487809	19.278999719968944	41.737376404993206
10	13.931203427762235	29.28271070060127	33.86884529308779	22.91724057854871
11	30.54785912844319	23.338539330703014	25.58186127307126	20.531740267782542
12	19.988525556817844	26.813201073680045	29.015429028064577	24.182844341437537
13	28.767612928783322	21.040577182705462	27.28185467071308	22.909955217798135
14	21.24923446795238	23.004551073793873	26.528047500552095	29.21816695770165
15	23.970430542053606	28.80460890134483	23.13215872069065	24.092801835910908
16	24.42189524106576	27.90418384607854	23.210590182521056	24.463330730334647
17	20.77773502687615	30.939105767501374	24.675175360909943	23.607983844712535
18	23.669567909807235	26.010445385976137	25.944421804174056	24.375564900042573
19	24.911949585303606	27.3131589551882	25.947381481978976	21.827509977529218
20	22.989638851007545	25.612141053690834	26.163324127976466	25.234895967325155
21	25.2543615405806	26.034350475938954	25.695581201037253	23.01570678244319
22	20.32934383943065	31.449308687565026	26.798516518417166	21.42283095458716
23	23.456698775376392	27.003986458335703	25.927915908723538	23.611398857564367
24	25.449017273135006	27.874359400505877	26.138280700396372	20.53834262596275
25	21.60929065629717	28.438405689866748	26.923733656317662	23.028569997518424
26	26.30914517674968	27.28777402632292	25.63479397227465	20.76828682465275
27	20.931182937685122	28.950999118926685	27.23973617887382	22.878081764514373
28	22.153416037355687	27.307581100863544	27.347992086276886	23.191010775503884
29	24.260365133174115	26.16480396687893	27.69757556854272	21.87725533140423
30	20.340727215603422	28.47016530938878	28.18922358544476	22.999883889563037
31	25.928143576246992	26.430378132989706	25.781411857379954	21.860066433383345
32	21.39277884149104	26.651215630741493	30.9059801428386	21.050025384928865
33	20.636581162333776	29.596436547922877	28.66778071974811	21.09920156999524
34	22.696972249605565	28.412679259716278	26.61091847908988	22.27943001158828
35	20.983546468079876	26.314495363550883	32.09759196060441	20.60436620776483
36	22.337940565116327	28.433283170588997	25.417371487374695	23.81140477691998
37	22.320979334618897	27.16528889870389	27.875953073170066	22.637778693507148
38	22.046412301331628	25.399727254306903	28.630329412139687	23.923531032221785
39	24.028599594296473	25.855289968741246	26.81581925019978	23.3002911867625
40	20.960552048210875	25.242408995599185	31.799461338639507	21.997577617550434
41	22.20122621728133	26.727484251099064	26.596347757588727	24.474941774030874
42	23.071940660736686	25.054014119939804	29.593818371403135	22.28022684792037
43	22.838695282956582	24.840006647891684	29.455282683380496	22.866015385771234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	12.0
2	15.0
3	77.5
4	140.0
5	140.0
6	185.0
7	230.0
8	239.0
9	248.0
10	398.5
11	549.0
12	549.0
13	852.0
14	1155.0
15	2907.5
16	4660.0
17	5265.0
18	5870.0
19	5870.0
20	6129.0
21	6388.0
22	7816.5
23	9245.0
24	11860.0
25	14475.0
26	14475.0
27	18200.5
28	21926.0
29	27818.0
30	33710.0
31	36562.0
32	39414.0
33	39414.0
34	42847.5
35	46281.0
36	48638.5
37	50996.0
38	54960.0
39	58924.0
40	58924.0
41	61763.0
42	64602.0
43	60856.0
44	57110.0
45	58843.5
46	60577.0
47	60577.0
48	62305.5
49	64034.0
50	79231.0
51	94428.0
52	78427.0
53	62426.0
54	62426.0
55	63956.5
56	65487.0
57	55096.5
58	44706.0
59	34194.5
60	23683.0
61	23683.0
62	20598.5
63	17514.0
64	14581.0
65	11648.0
66	9631.0
67	7614.0
68	7614.0
69	6096.0
70	4578.0
71	3702.0
72	2826.0
73	2208.0
74	1590.0
75	1590.0
76	1149.0
77	708.0
78	550.5
79	393.0
80	272.0
81	151.0
82	151.0
83	116.0
84	81.0
85	66.0
86	51.0
87	36.5
88	22.0
89	22.0
90	15.5
91	9.0
92	5.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	878474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.75296559554333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24206801669027	38.79549678911802
2	11.17116837866039	10.669128384963823
3	3.019031568235092	4.325031318293687
4	1.2467218462663527	2.381386617278776
5	0.6946468214252025	1.6585722882285614
6	0.4222622775578518	1.209856560750952
7	0.2896347675305937	0.9681643362413141
8	0.21703996983910395	0.8291441770067595
9	0.1697610532710062	0.7295934362682203
>10	1.2358250743158115	11.867245611296823
>50	0.16430975709804163	5.4450600560574225
>100	0.11582823505383105	10.187906877892486
>500	0.00764227530218318	2.509541724136878
>1k	0.0031046743415119164	2.692468555562261
>5k	2.3882110319322437E-4	0.881675395105758
>10k+	7.164633095796732E-4	4.849727871798261
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	16883	1.9218553992491525	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	15126	1.7218494798935426	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	10516	1.1970758383287383	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	7731	0.8800488119170289	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	3772	0.4293809492369723	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3326	0.3786110915064077	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2814	0.32032820550181335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2516	0.28640574450695183	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1908	0.21719481737649607	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1361	0.15492774971143142	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1307	0.14878072657813435	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1255	0.14286137096829274	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1158	0.1318194960807036	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1085	0.12350963147457977	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	1080	0.12294046266594115	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1023	0.11645193824746095	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1004	0.1142890967746342	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.11258159034871834	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	972	0.11064641639934704	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	930	0.10586539840678266	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	910	0.10358872317222821	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	888	0.1010843804142183	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	886	0.10085671289076284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	1.138337617277233E-4	0.0
20	0.0	0.0	0.0	1.138337617277233E-4	0.0
21	0.0	0.0	0.0	5.691688086386165E-4	0.0
22	0.0	0.0	0.0	0.0010245038555495097	0.0
23	0.0	0.0	0.0	0.0012521713790049564	0.0
24	0.0	0.0	0.0	0.0020490077110990193	0.0
25	0.0	0.0	0.0	0.0025043427580099128	0.0
26	0.0	0.0	0.0	0.0028458440431930825	0.0
27	0.0	0.0	0.0	0.003756514137014869	0.0
28	0.0	0.0	0.0	0.008082197082668354	0.0
29	0.0	0.0	0.0	0.018099568114708003	0.0
30	0.0	0.0	0.0	0.03198728704549025	0.0
31	0.0	0.0	0.0	0.07080459979464389	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	25	1.2333888E-4	37.0	5
CTTATAC	2080	0.0	33.17548	1
GATTACG	95	0.0	31.157896	1
TTATACA	2210	0.0	31.056559	2
TATACAC	2260	0.0	30.696903	3
GGTATCA	585	0.0	30.042736	1
CGCCGTG	25	0.0054956465	29.6	37
CGTCTTC	550	0.0	28.92727	37
TTACGGG	125	0.0	25.16	3
CCGTCTT	1665	0.0	24.666666	37
GCCGTCT	1755	0.0	23.401709	36
CGAGTAA	40	0.0019308263	23.125002	21
TGTTACG	40	0.0019308263	23.125002	17
GTATCAA	1635	0.0	23.082567	1
ATTACGG	130	0.0	22.76923	2
ACGGTAC	145	0.0	21.689655	3
CGACACG	70	5.099664E-6	21.142857	21
TCGTACT	70	5.099664E-6	21.142857	11
CACGGTA	155	0.0	20.290321	2
GCGGGTA	55	5.141766E-4	20.181818	22
>>END_MODULE