FastQCFastQC Report
Fri 10 Feb 2017
ERR1630983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977609
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT212102.169579044382775No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG162451.661707287882988No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT127681.3060436227571555RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC84750.8669110042972191No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC47360.4844472585665639RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC37550.3841003918744611No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23700.24242821005125773No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC22290.2280052659089677No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT20680.21153651408692023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.20273954106396322No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA19660.20110289492015726No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG19080.19517005264886064No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18640.19066927575339424No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT17760.18166772196246148No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC17750.1815654315784736No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT16240.16611958359630485No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG16190.1656081316763655No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA15080.15425389905371165No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA15050.15394702790174802No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA13880.14197905297516697No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13470.1377851472316642No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG12910.13205688572834334No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12860.13154543380840397No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT12220.12499884923318015No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA12180.12458968769722865No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT12120.12397594539330142No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC11710.11978203964979864No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG11640.11906600696188353No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC11060.11313316469058693No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10660.10904154933107203No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT10360.10597283781143586No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG9980.10208580321989671No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG9860.10085831861204224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25000.033.2261
TTACGGG800.032.3753
CGTCTTC6000.031.14166637
TTATACA27000.030.8333322
TATACAC27550.030.2177873
GGTATCA4600.029.760871
GATTACG905.456968E-1226.7222211
AACCACG358.8684453E-426.4285735
CGACTGC358.8684453E-426.4285725
TGTACGA358.8684453E-426.428572
CCGTCTT19900.026.03015137
GCCGTCT20150.025.79900636
GTATCAA14300.024.0629371
GTACGGT400.001930981623.12500211
CGCCGAG705.1006373E-621.1428572
TGCCGTC25300.020.54743235
GTCGCCA2950.020.06779912
TCGCCAT3000.019.73333413
ATGCCGT26550.019.4406834
TACACAT45700.018.6619265
Produced by FastQC (version 0.11.5)