##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630983.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977609
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.493894798431683	31.0	31.0	34.0	28.0	34.0
2	31.50944293679784	31.0	31.0	34.0	28.0	34.0
3	30.989916213946476	31.0	31.0	34.0	27.0	34.0
4	35.13747009284898	37.0	35.0	37.0	32.0	37.0
5	35.3213656993747	37.0	35.0	37.0	33.0	37.0
6	35.50604689604944	37.0	35.0	37.0	33.0	37.0
7	35.511349629555376	37.0	35.0	37.0	33.0	37.0
8	35.53430359172226	37.0	35.0	37.0	33.0	37.0
9	37.31162560901137	39.0	37.0	39.0	34.0	39.0
10	37.153298506867266	39.0	37.0	39.0	33.0	39.0
11	37.20646802556032	39.0	37.0	39.0	33.0	39.0
12	37.14227262637721	39.0	37.0	39.0	33.0	39.0
13	37.21510747139194	39.0	37.0	39.0	34.0	39.0
14	38.356752034811464	40.0	38.0	41.0	34.0	41.0
15	38.37917613278928	40.0	38.0	41.0	34.0	41.0
16	38.37022265547883	40.0	38.0	41.0	34.0	41.0
17	38.34573536045597	40.0	38.0	41.0	34.0	41.0
18	38.38556928178853	40.0	38.0	41.0	34.0	41.0
19	38.404636209363865	40.0	38.0	41.0	34.0	41.0
20	38.40585141912564	40.0	38.0	41.0	34.0	41.0
21	38.366196505965064	40.0	38.0	41.0	34.0	41.0
22	38.30530508618476	40.0	38.0	41.0	34.0	41.0
23	38.31250019179447	40.0	38.0	41.0	34.0	41.0
24	38.24946988008498	40.0	38.0	41.0	34.0	41.0
25	38.21170733902818	40.0	38.0	41.0	34.0	41.0
26	38.10554833271789	40.0	37.0	41.0	33.0	41.0
27	38.04823707637716	40.0	37.0	41.0	33.0	41.0
28	38.033039794028085	40.0	37.0	41.0	33.0	41.0
29	37.98509935976449	40.0	37.0	41.0	33.0	41.0
30	37.90593990030779	40.0	37.0	41.0	33.0	41.0
31	37.88436378961323	40.0	37.0	41.0	33.0	41.0
32	37.79604013465506	40.0	37.0	41.0	33.0	41.0
33	37.778980144413566	40.0	37.0	41.0	33.0	41.0
34	37.75174226096527	40.0	37.0	41.0	33.0	41.0
35	37.719604668123964	40.0	37.0	41.0	33.0	41.0
36	37.69205479900451	40.0	37.0	41.0	33.0	41.0
37	37.66246730543602	40.0	37.0	41.0	33.0	41.0
38	37.62185802299283	40.0	37.0	41.0	33.0	41.0
39	37.54282233490076	40.0	37.0	41.0	32.0	41.0
40	37.4633560042921	40.0	37.0	41.0	32.0	41.0
41	37.44551144680542	40.0	37.0	41.0	32.0	41.0
42	37.41698163580736	40.0	37.0	41.0	32.0	41.0
43	36.75944472687956	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	7.0
11	9.0
12	7.0
13	4.0
14	7.0
15	5.0
16	3.0
17	7.0
18	18.0
19	39.0
20	66.0
21	183.0
22	298.0
23	578.0
24	1092.0
25	1974.0
26	3217.0
27	5053.0
28	7509.0
29	10736.0
30	15323.0
31	20607.0
32	27215.0
33	35291.0
34	47075.0
35	63637.0
36	88299.0
37	131050.0
38	234079.0
39	284208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.44994164333593	22.96930572447676	15.20505641826129	24.37569621392602
2	13.13572195018663	22.201820973415753	42.26280650034932	22.3996505760483
3	14.190949551405522	29.0536400544594	35.640833912126425	21.11457648200866
4	11.579885209731088	19.815590895746663	37.40493387438127	31.199590020140977
5	11.740378822208061	39.41268953129523	36.14850108785823	12.698430558638474
6	26.707098645777606	40.457278932579385	18.209222705601114	14.626399716041893
7	22.959895009149875	34.455288361706984	23.035385312532924	19.549431316610217
8	23.46838050795359	36.78904347239029	22.51534100033858	17.22723501931754
9	22.94178961118402	15.786168089696393	19.431285923104227	41.84075637601536
10	13.54529264767407	29.784914009588704	34.22758996694998	22.442203375787255
11	29.913595312645448	23.456003371491054	26.43541538590582	20.19498592995768
12	19.334416929467714	27.173542796762305	29.267426956994054	24.22461331677593
13	27.854080721433622	21.079593170684806	28.109192939099376	22.9571331687822
14	20.769960178353514	23.25878751116244	26.502927039337816	29.468325271146234
15	23.56944340733361	29.34946384495233	23.383581779627644	23.697510968086423
16	24.700263602319534	28.32338900316998	23.4134505717521	23.562896822758383
17	20.63882390608106	31.598829388845644	24.757239346200784	23.005107358872515
18	22.70437362994817	26.9702918037784	26.017252296163395	24.308082270110035
19	23.551644880519717	29.258936855123057	26.13457936659748	21.05483889775974
20	23.756021067727488	25.97183536567278	26.54005844872541	23.732085117874323
21	24.015020319984778	27.078105868501616	25.191359735845314	23.71551407566829
22	20.08359170179489	32.154061593131814	25.6376526811844	22.124694023888896
23	22.76585015072488	27.911465626850816	25.778301959167727	23.544382263256576
24	25.093876999904868	29.67341749104192	25.022785183033296	20.209920326019912
25	22.501122636964265	30.113266142189772	25.677750511707647	21.707860709138316
26	24.063915123530982	29.583095081980627	25.350728154098416	21.00226164038997
27	22.005525726543024	30.01670401970522	26.230527746778108	21.747242506973645
28	21.385748289960503	28.104794452587896	27.445942089322013	23.06351516812959
29	21.938218653879005	29.434160282894283	25.674477219420034	22.953143843806675
30	22.315567880410267	29.72763139455549	27.057340920552083	20.89945980448216
31	25.60491975830828	26.825039458515626	25.767561468849	21.802479314327098
32	21.254509727304065	28.9676138415256	26.93888865589413	22.83898777527621
33	20.37736968460806	30.28501169690541	28.351007406846705	20.986611211639826
34	22.484040142838293	29.43876335017374	26.284230198371745	21.792966308616226
35	20.89015137953926	28.954418381991164	29.78563004227662	20.369800196192955
36	22.029563966780174	29.278985770384686	25.01501111385022	23.676439148984922
37	22.14709561798224	27.634258686243683	27.575953167370592	22.642692528403483
38	21.987727199729136	26.095913601450064	28.643762485819995	23.2725967130008
39	23.508069176940882	26.43040315709041	26.984510167152713	23.077017498815987
40	20.870920787349544	25.218671268370073	32.015560413212235	21.894847531068145
41	21.621936786588503	27.334240990007252	26.649304578824456	24.394517644579786
42	23.034464699077034	25.372516005887835	29.669223585298415	21.923795709736716
43	22.226984407876767	25.482887330210747	29.649583831572745	22.640544430339737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	14.5
2	19.0
3	86.5
4	154.0
5	154.0
6	207.5
7	261.0
8	257.0
9	253.0
10	424.0
11	595.0
12	595.0
13	835.0
14	1075.0
15	2837.5
16	4600.0
17	5236.0
18	5872.0
19	5872.0
20	6543.0
21	7214.0
22	9385.5
23	11557.0
24	14526.5
25	17496.0
26	17496.0
27	21627.5
28	25759.0
29	33683.5
30	41608.0
31	43412.0
32	45216.0
33	45216.0
34	49253.5
35	53291.0
36	55823.5
37	58356.0
38	63951.0
39	69546.0
40	69546.0
41	73917.0
42	78288.0
43	72231.0
44	66174.0
45	67501.0
46	68828.0
47	68828.0
48	70155.5
49	71483.0
50	88952.5
51	106422.0
52	86805.0
53	67188.0
54	67188.0
55	67023.0
56	66858.0
57	54736.5
58	42615.0
59	32350.0
60	22085.0
61	22085.0
62	19195.5
63	16306.0
64	13496.0
65	10686.0
66	8956.0
67	7226.0
68	7226.0
69	5764.5
70	4303.0
71	3617.0
72	2931.0
73	2303.5
74	1676.0
75	1676.0
76	1257.0
77	838.0
78	650.0
79	462.0
80	326.0
81	190.0
82	190.0
83	134.5
84	79.0
85	65.5
86	52.0
87	40.0
88	28.0
89	28.0
90	18.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	977609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.88383390135535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2878256997941	35.23337607310734
2	11.674875647935098	10.246766075059245
3	3.196728725529459	4.208541372564785
4	1.3625097808547082	2.391686116480004
5	0.7400071773605293	1.623717602855014
6	0.44868558332148595	1.1814026167447698
7	0.3144444134498454	0.9659318487729489
8	0.23400167804113786	0.8215112617436581
9	0.17211090890345485	0.6797597885037463
>10	1.2593415039110034	11.072456265828636
>50	0.15274967876303813	4.756732005064335
>100	0.13803434942413212	12.509252619603512
>500	0.011444472286080802	3.455631453845149
>1k	0.006306137790289422	4.836961577038715
>5k	2.3356065889960822E-4	0.8686482743982689
>10k+	7.006819766988248E-4	5.147625048389883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	21210	2.169579044382775	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	16245	1.661707287882988	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	12768	1.3060436227571555	RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	8475	0.8669110042972191	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	4736	0.4844472585665639	RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	3755	0.3841003918744611	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2370	0.24242821005125773	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2229	0.2280052659089677	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2068	0.21153651408692023	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1982	0.20273954106396322	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1966	0.20110289492015726	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1908	0.19517005264886064	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1864	0.19066927575339424	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1776	0.18166772196246148	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1775	0.1815654315784736	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1624	0.16611958359630485	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1619	0.1656081316763655	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1508	0.15425389905371165	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	1505	0.15394702790174802	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1388	0.14197905297516697	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1347	0.1377851472316642	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1291	0.13205688572834334	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1286	0.13154543380840397	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1222	0.12499884923318015	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1218	0.12458968769722865	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	1212	0.12397594539330142	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1171	0.11978203964979864	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	1164	0.11906600696188353	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1106	0.11313316469058693	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	1066	0.10904154933107203	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1036	0.10597283781143586	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	998	0.10208580321989671	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	986	0.10085831861204224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0229038398787245E-4	0.0
8	0.0	0.0	0.0	2.045807679757449E-4	0.0
9	0.0	0.0	0.0	3.0687115196361737E-4	0.0
10	0.0	0.0	0.0	5.114519199393622E-4	0.0
11	0.0	0.0	0.0	6.137423039272347E-4	0.0
12	0.0	0.0	0.0	6.137423039272347E-4	0.0
13	0.0	0.0	0.0	6.137423039272347E-4	0.0
14	0.0	0.0	0.0	6.137423039272347E-4	0.0
15	0.0	0.0	0.0	6.137423039272347E-4	0.0
16	0.0	0.0	0.0	7.160326879151072E-4	0.0
17	0.0	0.0	0.0	8.183230719029796E-4	0.0
18	0.0	0.0	0.0	9.206134558908521E-4	0.0
19	0.0	0.0	0.0	9.206134558908521E-4	0.0
20	0.0	0.0	0.0	0.0010229038398787244	0.0
21	0.0	0.0	0.0	0.001329774991842342	0.0
22	0.0	0.0	0.0	0.0016366461438059592	0.0
23	0.0	0.0	0.0	0.0018412269117817042	0.0
24	0.0	0.0	0.0	0.002659549983684684	0.0
25	0.0	0.0	0.0	0.002659549983684684	0.0
26	0.0	0.0	0.0	0.0027618403676725562	0.0
27	0.0	0.0	0.0	0.003171001903624046	0.0
28	0.0	0.0	0.0	0.007058036495163199	0.0
29	0.0	0.0	0.0	0.016468751822047465	0.0
30	0.0	0.0	0.0	0.029766501740470883	0.0
31	0.0	0.0	0.0	0.07344449570329242	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2500	0.0	33.226	1
TTACGGG	80	0.0	32.375	3
CGTCTTC	600	0.0	31.141666	37
TTATACA	2700	0.0	30.833332	2
TATACAC	2755	0.0	30.217787	3
GGTATCA	460	0.0	29.76087	1
GATTACG	90	5.456968E-12	26.722221	1
AACCACG	35	8.8684453E-4	26.42857	35
CGACTGC	35	8.8684453E-4	26.42857	25
TGTACGA	35	8.8684453E-4	26.42857	2
CCGTCTT	1990	0.0	26.030151	37
GCCGTCT	2015	0.0	25.799006	36
GTATCAA	1430	0.0	24.062937	1
GTACGGT	40	0.0019309816	23.125002	11
CGCCGAG	70	5.1006373E-6	21.142857	2
TGCCGTC	2530	0.0	20.547432	35
GTCGCCA	295	0.0	20.067799	12
TCGCCAT	300	0.0	19.733334	13
ATGCCGT	2655	0.0	19.44068	34
TACACAT	4570	0.0	18.661926	5
>>END_MODULE