FastQCFastQC Report
Fri 10 Feb 2017
ERR1630982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999188
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT198611.9877140237873152No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG141611.4172508076558166No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT105401.0548565435133328TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC73710.7376990115974171No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC36850.3687994651657146RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC31910.31935931976765136TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24790.248101458384208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.2136735028843421No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG18880.18895343018531047No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17870.17884522232052427No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC17380.17394124028711314No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15460.15472563721742055No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC14870.14882084252412958No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14650.14661905467239397No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA14490.14501775441658626No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT13760.13771182199946358No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA13420.13430905895587217No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT13150.13160686477419664No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA11490.11499337462019159No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT11190.11199093664055212No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG10840.10848809233097276No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC10830.10838801106498477No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA10630.10638638574522513No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10360.10368419156354959No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10100.10108207864786206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23300.032.950641
TTATACA25750.029.9592232
GATTACG1050.029.952381
TATACAC26450.029.6559543
CGTCTTC5700.029.5350937
TTACGGG1100.028.5909083
GGTATCA4900.027.9387741
TGGACCG358.8685815E-426.428575
CCGTCTT17100.025.6403537
GCCGTCT17450.025.02005836
GGTCTAT451.3230431E-424.6666661
GTATCAA15050.023.1096361
TGCCGTC20750.021.04096435
ATGCCGT21450.020.35431134
TTAACGG555.142447E-420.18181835
GTCTAGA656.900979E-519.9230751
ATTACGG1600.019.656252
CGAATTA951.6755621E-719.47368415
CCGAATT1052.2584572E-819.3809514
TATGCCG22750.019.35384833
Produced by FastQC (version 0.11.5)