##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630982.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 999188 Sequences flagged as poor quality 0 Sequence length 43 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.487734040040515 31.0 31.0 34.0 28.0 34.0 2 31.494239322329733 31.0 31.0 34.0 28.0 34.0 3 30.96459425053143 31.0 31.0 34.0 27.0 34.0 4 35.1229408279523 37.0 35.0 37.0 32.0 37.0 5 35.306705044496134 37.0 35.0 37.0 33.0 37.0 6 35.49687145962522 37.0 35.0 37.0 33.0 37.0 7 35.50291937052887 37.0 35.0 37.0 33.0 37.0 8 35.52167159733704 37.0 35.0 37.0 33.0 37.0 9 37.29770673787115 39.0 37.0 39.0 34.0 39.0 10 37.134521231239766 39.0 37.0 39.0 33.0 39.0 11 37.203881551820075 39.0 37.0 39.0 33.0 39.0 12 37.13575223081142 39.0 37.0 39.0 33.0 39.0 13 37.20188993462692 39.0 37.0 39.0 33.0 39.0 14 38.34238401582085 40.0 38.0 41.0 34.0 41.0 15 38.35611316388908 40.0 38.0 41.0 34.0 41.0 16 38.35083187548289 40.0 38.0 41.0 34.0 41.0 17 38.31692434256616 40.0 38.0 41.0 33.0 41.0 18 38.354211619835304 40.0 38.0 41.0 34.0 41.0 19 38.382293422258876 40.0 38.0 41.0 34.0 41.0 20 38.354814109056555 40.0 38.0 41.0 34.0 41.0 21 38.30836739432419 40.0 38.0 41.0 34.0 41.0 22 38.22371465630091 40.0 38.0 41.0 34.0 41.0 23 38.24211759949079 40.0 38.0 41.0 34.0 41.0 24 38.20081906508084 40.0 38.0 41.0 33.0 41.0 25 38.15779412883261 40.0 38.0 41.0 33.0 41.0 26 38.07795730132868 40.0 37.0 41.0 33.0 41.0 27 37.96746257961465 40.0 37.0 41.0 33.0 41.0 28 37.97281792815767 40.0 37.0 41.0 33.0 41.0 29 37.88207624591168 40.0 37.0 41.0 33.0 41.0 30 37.80094436682586 40.0 37.0 41.0 33.0 41.0 31 37.81018687173985 40.0 37.0 41.0 33.0 41.0 32 37.68280944126631 40.0 37.0 41.0 32.0 41.0 33 37.722130369860324 40.0 37.0 41.0 33.0 41.0 34 37.70411674279515 40.0 37.0 41.0 33.0 41.0 35 37.693899446350436 40.0 37.0 41.0 33.0 41.0 36 37.6785589898998 40.0 37.0 41.0 33.0 41.0 37 37.62953618338091 40.0 37.0 41.0 33.0 41.0 38 37.57238377562581 40.0 37.0 41.0 32.0 41.0 39 37.512177888445414 40.0 37.0 41.0 32.0 41.0 40 37.439095545583015 39.0 37.0 41.0 32.0 41.0 41 37.40413015368479 39.0 37.0 41.0 32.0 41.0 42 37.368565275003306 39.0 37.0 41.0 31.0 41.0 43 36.67875014511784 39.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 19.0 11 12.0 12 16.0 13 8.0 14 11.0 15 17.0 16 7.0 17 20.0 18 19.0 19 32.0 20 73.0 21 182.0 22 342.0 23 656.0 24 1258.0 25 1972.0 26 3412.0 27 5306.0 28 7889.0 29 11498.0 30 15867.0 31 21505.0 32 28245.0 33 36616.0 34 48573.0 35 65583.0 36 90568.0 37 135945.0 38 241949.0 39 281582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.064508380805215 22.24916632305432 15.109568970003643 24.576756326136824 2 13.392674851979807 22.192420245239134 41.96507564142083 22.449829261360225 3 14.55912200706974 28.477723911816394 35.07398007181832 21.889174009295548 4 11.744536563689715 19.73892800954375 37.45431290207649 31.062222524690046 5 12.052987025464677 38.71293490314135 36.28766558445457 12.946412486939396 6 27.28145253946204 40.15520602729416 18.033543237108532 14.529798196135262 7 23.675924850978994 33.65032406313927 22.965047618666357 19.70870346721538 8 23.768099696953925 36.505042094180475 22.253870142555755 17.472988066309846 9 23.59235699387903 15.620483832872292 19.36842716285624 41.41873201039244 10 13.704327914266385 29.730140874390003 34.006513288790494 22.559017922553114 11 30.516479381257582 23.505486455001463 25.902432775413637 20.07560138832732 12 19.967313458528324 26.650840482471764 29.414284398931933 23.967561660067975 13 28.073495678490936 21.211023350960982 27.7132031209342 23.002277849613886 14 21.151775241496097 22.573429624855383 26.592593185666765 29.682201947981763 15 23.603966420733634 28.96742154629559 23.4335280247561 23.995084008214672 16 24.469068883933755 28.219013839237462 23.39589746874462 23.916019808084165 17 20.98834253413772 30.889382178328802 24.878701505622566 23.24357378191091 18 23.468156142787944 26.487607937645368 26.11900863501163 23.92522728455506 19 25.008306745077004 27.36892356593554 26.12201107299127 21.500758615996187 20 23.637693807371583 26.27073183424941 26.340988882972976 23.75058547540603 21 23.695841022910606 27.751033839477657 25.230987561900264 23.322137575711476 22 21.843336789473053 30.935719804481238 25.564358258906232 21.656585147139477 23 22.347145882456555 26.646737150566256 26.224694451894937 24.781422515082248 24 24.777219101910752 28.335508432847472 26.47219542268322 20.415077042558558 25 22.472247464941532 29.083515814841654 25.713579426494316 22.730657293722505 26 24.879702318282444 26.960391838172598 25.818064268185765 22.341841575359194 27 23.387190398603668 29.011056978266353 26.138624563145274 21.463128059984708 28 20.274362782579455 27.832199746193908 27.46199914330436 24.431438327922272 29 24.917633118091892 26.827583998206546 26.5414516587469 21.71333122495466 30 22.58403823905011 27.69418768039648 26.8970403968022 22.824733683751205 31 22.019780061409865 27.970712218321275 27.350808856791716 22.658698863477145 32 23.579546591832568 28.288270075301142 26.841495294178873 21.290688038687414 33 20.6995080004964 25.924750897728956 28.27485918565876 25.100881916115885 34 20.637857940647805 28.463212128248138 28.568497620067497 22.33043231103656 35 21.179998158504706 28.466214566227777 29.296788992662044 21.056998282605477 36 22.328230523184825 26.571576119809286 25.433451962993953 25.66674139401194 37 22.468144133036024 27.48131482764004 27.425569562484736 22.624971476839193 38 22.2501671357142 25.935359511923682 28.470518060665263 23.343955291696858 39 23.69403956012282 26.252116718775646 26.675560555170797 23.378283165930736 40 21.14747174705861 25.4238441614591 31.267188957433433 22.16149513404885 41 21.90238473640596 26.86681585447383 26.754824917833282 24.475974491286927 42 23.30062010352406 25.317557856979867 29.075909638626563 22.305912400869506 43 22.54780882076246 25.201263425901832 29.393967901936374 22.856959851399335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6.0 1 11.0 2 16.0 3 91.5 4 167.0 5 167.0 6 195.5 7 224.0 8 241.0 9 258.0 10 427.0 11 596.0 12 596.0 13 881.5 14 1167.0 15 2884.5 16 4602.0 17 5216.0 18 5830.0 19 5830.0 20 6391.5 21 6953.0 22 8994.5 23 11036.0 24 14022.0 25 17008.0 26 17008.0 27 21450.0 28 25892.0 29 32847.5 30 39803.0 31 42963.0 32 46123.0 33 46123.0 34 50087.0 35 54051.0 36 56779.5 37 59508.0 38 64277.5 39 69047.0 40 69047.0 41 72800.5 42 76554.0 43 71327.0 44 66100.0 45 67335.0 46 68570.0 47 68570.0 48 68080.0 49 67590.0 50 69449.0 51 71308.0 52 88208.0 53 105108.0 54 105108.0 55 77953.5 56 50799.0 57 54142.0 58 57485.0 59 46618.5 60 35752.0 61 35752.0 62 28129.0 63 20506.0 64 17161.5 65 13817.0 66 11547.0 67 9277.0 68 9277.0 69 7430.5 70 5584.0 71 4700.0 72 3816.0 73 3008.0 74 2200.0 75 2200.0 76 1667.0 77 1134.0 78 941.0 79 748.0 80 511.0 81 274.0 82 274.0 83 204.5 84 135.0 85 111.5 86 88.0 87 61.5 88 35.0 89 35.0 90 27.0 91 19.0 92 10.5 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 999188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.19786276378072 #Duplication Level Percentage of deduplicated Percentage of total 1 82.23430956251576 41.279865858934265 2 10.98122810811097 11.024683630974517 3 2.817347965430719 4.242745395795242 4 1.1205775468852879 2.25002391658887 5 0.5969047928222676 1.4981672436567575 6 0.37909496940270415 1.1417854349109957 7 0.256187775634083 0.9002055162126564 8 0.20201970750440224 0.8112766042308083 9 0.1616689634860896 0.7303892798013661 >10 1.0086666550469834 9.875008447210968 >50 0.1242962521254155 4.3769819029389545 >100 0.10530846937062133 10.668314832389326 >500 0.007393573735403867 2.537906382190063 >1k 0.004196352660634627 3.4533221224322084 >5k 1.9982631717307747E-4 0.7393742517340404 >10k+ 5.994789515192324E-4 4.469949179998957 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT 19861 1.9877140237873152 No Hit ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG 14161 1.4172508076558166 No Hit TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT 10540 1.0548565435133328 TruSeq Adapter, Index 11 (95% over 23bp) ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC 7371 0.7376990115974171 No Hit CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC 3685 0.3687994651657146 RNA PCR Primer, Index 11 (95% over 24bp) CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC 3191 0.31935931976765136 TruSeq Adapter, Index 11 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2479 0.248101458384208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2135 0.2136735028843421 No Hit CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG 1888 0.18895343018531047 No Hit GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT 1787 0.17884522232052427 No Hit GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC 1738 0.17394124028711314 No Hit GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC 1546 0.15472563721742055 No Hit GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC 1487 0.14882084252412958 No Hit GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG 1465 0.14661905467239397 No Hit ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA 1449 0.14501775441658626 No Hit ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT 1376 0.13771182199946358 No Hit TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA 1342 0.13430905895587217 No Hit GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT 1315 0.13160686477419664 No Hit ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA 1149 0.11499337462019159 No Hit TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT 1119 0.11199093664055212 No Hit CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG 1084 0.10848809233097276 No Hit CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC 1083 0.10838801106498477 No Hit ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA 1063 0.10638638574522513 No Hit TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA 1036 0.10368419156354959 No Hit CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC 1010 0.10108207864786206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0008126598798224E-4 0.0 0.0 0.0 3 0.0 1.0008126598798224E-4 0.0 0.0 0.0 4 0.0 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 5 0.0 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 6 0.0 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 7 0.0 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 8 0.0 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 9 1.0008126598798224E-4 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 10 3.002437979639467E-4 1.0008126598798224E-4 0.0 1.0008126598798224E-4 0.0 11 3.002437979639467E-4 1.0008126598798224E-4 0.0 2.0016253197596449E-4 0.0 12 3.002437979639467E-4 1.0008126598798224E-4 0.0 3.002437979639467E-4 0.0 13 3.002437979639467E-4 1.0008126598798224E-4 0.0 3.002437979639467E-4 0.0 14 3.002437979639467E-4 1.0008126598798224E-4 0.0 3.002437979639467E-4 0.0 15 3.002437979639467E-4 1.0008126598798224E-4 0.0 3.002437979639467E-4 0.0 16 3.002437979639467E-4 1.0008126598798224E-4 0.0 4.0032506395192897E-4 0.0 17 3.002437979639467E-4 1.0008126598798224E-4 0.0 4.0032506395192897E-4 0.0 18 3.002437979639467E-4 1.0008126598798224E-4 0.0 4.0032506395192897E-4 0.0 19 4.0032506395192897E-4 1.0008126598798224E-4 0.0 4.0032506395192897E-4 0.0 20 5.004063299399112E-4 1.0008126598798224E-4 0.0 6.004875959278934E-4 0.0 21 6.004875959278934E-4 1.0008126598798224E-4 0.0 8.006501279038579E-4 0.0 22 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.0011008939258678047 0.0 23 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.0019015440537716625 0.0 24 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.002001625319759645 0.0 25 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.002101706585747627 0.0 26 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.002101706585747627 0.0 27 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.0027021941816755203 0.0 28 6.004875959278934E-4 1.0008126598798224E-4 0.0 0.007506094949098668 0.0 29 7.005688619158757E-4 1.0008126598798224E-4 0.0 0.019015440537716626 0.0 30 7.005688619158757E-4 1.0008126598798224E-4 0.0 0.035128524361781766 0.0 31 7.005688619158757E-4 1.0008126598798224E-4 0.0 0.07686241227877036 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 2330 0.0 32.95064 1 TTATACA 2575 0.0 29.959223 2 GATTACG 105 0.0 29.95238 1 TATACAC 2645 0.0 29.655954 3 CGTCTTC 570 0.0 29.53509 37 TTACGGG 110 0.0 28.590908 3 GGTATCA 490 0.0 27.938774 1 TGGACCG 35 8.8685815E-4 26.42857 5 CCGTCTT 1710 0.0 25.64035 37 GCCGTCT 1745 0.0 25.020058 36 GGTCTAT 45 1.3230431E-4 24.666666 1 GTATCAA 1505 0.0 23.109636 1 TGCCGTC 2075 0.0 21.040964 35 ATGCCGT 2145 0.0 20.354311 34 TTAACGG 55 5.142447E-4 20.181818 35 GTCTAGA 65 6.900979E-5 19.923075 1 ATTACGG 160 0.0 19.65625 2 CGAATTA 95 1.6755621E-7 19.473684 15 CCGAATT 105 2.2584572E-8 19.38095 14 TATGCCG 2275 0.0 19.353848 33 >>END_MODULE