FastQCFastQC Report
Fri 10 Feb 2017
ERR1630981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622144
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG135062.170880053492439No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT129972.0890661968933237No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT118181.899560230428968TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC71031.1416970990638824No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC43240.6950159448616398RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC33000.530423824709392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.31584327744059254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17930.28819694475876967No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG16170.25990767410760207No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA12190.19593534615780273No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT10850.17439692418475464No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9290.14932234338031067No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8880.1427322291945273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7580.12183674519082399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16100.033.7826081
CGTCTTC6150.031.28455537
GATTACG551.8528226E-830.2727281
TTATACA19350.028.5865632
TTACGGG652.9849616E-928.4615383
TATACAC19500.028.4615383
CCGTCTT18200.024.59890237
GTATCAA7850.024.2738861
GCCGTCT18750.023.6836
GGTATCA2650.022.3396221
TGCCGTC21550.020.51740335
ATGCCGT23050.019.1822134
TTAACGG609.2303246E-418.535
TATAGAC500.007031342518.53
GTTATAC609.2303246E-418.53
CCGTACA2200.017.6590917
TATGCCG25150.017.65407633
AACCGTT1054.788526E-717.6190510
ACCGTTA1054.788526E-717.6190511
GCAACTT650.001578832617.07692313
Produced by FastQC (version 0.11.5)