FastQCFastQC Report
Fri 10 Feb 2017
ERR1630980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869280
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40420.46498251426467885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39570.4552043070127002No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT33350.3836508374746917No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG31760.36535983802687283No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT20290.23341155899134916No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC19660.22616418185164733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12680.1458678446530462No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10340.11894901527701085No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9680.11135652494018039No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC8890.10226854408245906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG1150.032.1739121
GGTATCA5450.027.1559661
ATTACGG1400.026.428572
TCGACTA358.8676496E-426.4285727
GCCCGAT358.8676496E-426.4285712
GCAACGC509.087764E-625.8999983
GTATCAA17950.025.5598871
TTACGGG1550.025.0645163
CTTATAC5850.024.6666681
TATAACG451.3228496E-424.6666662
CGTCTTC1350.023.29629737
ACCGATG400.001930810523.12527
CGCCCGA400.001930810523.12511
CCGTCTT3250.022.7692337
GCCGTCT3350.022.08955236
TTATACA6800.021.7647062
TATACAC7100.021.1056353
CGAACGC450.00382506920.55555530
ACTACGC450.00382506920.55555530
ATGACCG450.00382506920.5555555
Produced by FastQC (version 0.11.5)