##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630980.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869280
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569317136020615	31.0	31.0	34.0	30.0	34.0
2	31.554098794404563	31.0	31.0	34.0	30.0	34.0
3	31.02582482054114	31.0	31.0	34.0	27.0	34.0
4	35.18141335357998	37.0	35.0	37.0	32.0	37.0
5	35.385083057242774	37.0	35.0	37.0	33.0	37.0
6	35.576676099760725	37.0	35.0	37.0	33.0	37.0
7	35.59118350819069	37.0	35.0	37.0	33.0	37.0
8	35.61061913307565	37.0	35.0	37.0	33.0	37.0
9	37.38036651021535	39.0	37.0	39.0	34.0	39.0
10	37.22805770292656	39.0	37.0	39.0	34.0	39.0
11	37.29102590649733	39.0	37.0	39.0	34.0	39.0
12	37.22986034419289	39.0	37.0	39.0	34.0	39.0
13	37.28788077489416	39.0	37.0	39.0	34.0	39.0
14	38.46937120375483	40.0	38.0	41.0	34.0	41.0
15	38.4680965856801	40.0	38.0	41.0	34.0	41.0
16	38.48418116142095	40.0	38.0	41.0	34.0	41.0
17	38.47778276274618	40.0	38.0	41.0	34.0	41.0
18	38.502536581998896	40.0	38.0	41.0	34.0	41.0
19	38.54860919381557	40.0	38.0	41.0	34.0	41.0
20	38.53363933370145	40.0	38.0	41.0	34.0	41.0
21	38.491113335173935	40.0	38.0	41.0	34.0	41.0
22	38.438966731087795	40.0	38.0	41.0	34.0	41.0
23	38.414067964292286	40.0	38.0	41.0	34.0	41.0
24	38.3945633167679	40.0	38.0	41.0	34.0	41.0
25	38.366303147432355	40.0	38.0	41.0	34.0	41.0
26	38.269082459046565	40.0	38.0	41.0	34.0	41.0
27	38.22083793484263	40.0	38.0	41.0	34.0	41.0
28	38.18066445794221	40.0	38.0	41.0	34.0	41.0
29	38.10539526964845	40.0	38.0	41.0	33.0	41.0
30	38.00625920301859	40.0	37.0	41.0	33.0	41.0
31	37.97239209460703	40.0	37.0	41.0	33.0	41.0
32	37.8870398490705	40.0	37.0	41.0	33.0	41.0
33	37.87886526780784	40.0	37.0	41.0	33.0	41.0
34	37.818582044910734	40.0	37.0	41.0	33.0	41.0
35	37.764734032762746	40.0	37.0	41.0	33.0	41.0
36	37.7378922786674	40.0	37.0	41.0	33.0	41.0
37	37.685474185532854	40.0	37.0	41.0	33.0	41.0
38	37.64045877047672	40.0	37.0	41.0	33.0	41.0
39	37.57000621203755	40.0	37.0	41.0	32.0	41.0
40	37.52362414872078	40.0	37.0	41.0	32.0	41.0
41	37.451831400699426	40.0	37.0	41.0	32.0	41.0
42	37.38228994110068	40.0	37.0	41.0	32.0	41.0
43	36.80796636296705	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	3.0
12	0.0
13	2.0
14	2.0
15	2.0
16	8.0
17	8.0
18	6.0
19	26.0
20	55.0
21	112.0
22	230.0
23	494.0
24	842.0
25	1491.0
26	2603.0
27	4003.0
28	6234.0
29	8943.0
30	12503.0
31	17009.0
32	22922.0
33	30181.0
34	40566.0
35	55278.0
36	77903.0
37	118536.0
38	208901.0
39	260412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.82040309221424	23.33747469169888	15.50110436223081	22.341017853856066
2	13.643244984354869	23.78347598012148	41.25368120743604	21.319597828087613
3	14.80708172280508	29.19669151481686	34.353487944045646	21.642738818332415
4	11.711186269096263	18.961669427572243	41.16441192711209	28.162732376219402
5	11.747538192527148	39.58540401251611	36.398628750230074	12.26842904472667
6	26.42278667402908	42.069413767715815	18.478510951592124	13.029288606662986
7	23.721240566905948	33.85042794036444	24.85091109884042	17.577420393889195
8	23.90495582551077	36.351003129026324	22.270384686177067	17.473656359285847
9	24.71884778207252	15.828616786305908	21.439697220688387	38.01283821093319
10	13.76610528253267	29.732997423154796	35.32567182035708	21.175225473955457
11	31.320633167678995	24.97204583103258	25.929159764402726	17.778161236885698
12	20.59278943493466	27.355167494938335	31.986816675869683	20.065226394257316
13	27.224484630958955	23.149157923799006	27.956009571139333	21.670347874102706
14	20.782371617890668	22.65702650469354	29.616119087060554	26.944482790355234
15	22.230121479845387	30.27148904840788	25.74855052457206	21.749838947174673
16	21.605811706239646	29.8771397018222	25.92823946254371	22.58880912939444
17	20.696323394073257	29.52800018406037	27.797832689122032	21.97784373274434
18	21.68783360942389	28.12994662249218	29.04392140622124	21.138298361862688
19	22.96578777839131	27.734331860850357	29.466800110436225	19.833080250322105
20	22.78264770844837	27.314789250874288	29.011480765691143	20.891082274986196
21	22.168806368488863	27.990175777655068	28.721930793300203	21.119087060555863
22	20.664803055402174	29.440916620651574	28.80993465856801	21.084345665378244
23	21.37930241119087	27.77908153874471	29.121341800110436	21.720274249953984
24	21.710265967237255	28.415355236517577	28.83087152586048	21.043507270384687
25	21.35928584575741	28.182058715258606	29.031497331124612	21.427158107859377
26	21.895131603165837	27.86605006442113	28.90978741027057	21.329030922142465
27	20.943079330020247	28.65118258788883	28.666712681759616	21.739025400331307
28	20.975289895085588	28.104523283637032	29.357744340143565	21.56244248113381
29	21.665516289342904	28.42651389655807	28.958218295600958	20.949751518498065
30	20.86991533222897	28.439168047119455	29.02505521811154	21.66586140254003
31	22.53934290447267	27.691767899871156	28.60942389103626	21.159465304619914
32	20.749470826431068	28.439513160316583	29.530646051905023	21.280369961347322
33	20.80940548499908	27.57442941284741	29.79454260997607	21.821622492177433
34	21.297625621203757	28.020545739002394	28.972252898950856	21.709575740842997
35	20.86140254003313	28.293990428860667	30.03991809313455	20.804688937971655
36	21.618810969998158	28.231639977912753	28.081285661697038	22.068263390392048
37	21.8067826247009	27.81945978280876	28.838003865267808	21.535753727222527
38	21.57981317872262	27.86915608319529	28.969491993373826	21.581538744708265
39	21.64526964844469	27.861218479661332	28.998941652862136	21.494570219031843
40	21.068930609239832	27.71120927664274	29.879440456469723	21.340419657647708
41	21.370329468065524	27.87329744156083	28.731018774157924	22.02535431621572
42	21.402309957666116	27.50172556598564	29.360045094791094	21.73591938155715
43	21.394832505061657	27.24381096999816	29.513620467513345	21.847736057426836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	31.5
2	46.0
3	151.5
4	257.0
5	257.0
6	322.0
7	387.0
8	403.5
9	420.0
10	665.5
11	911.0
12	911.0
13	1443.5
14	1976.0
15	4699.5
16	7423.0
17	8219.5
18	9016.0
19	9016.0
20	9447.0
21	9878.0
22	12486.0
23	15094.0
24	18775.5
25	22457.0
26	22457.0
27	27115.5
28	31774.0
29	37213.0
30	42652.0
31	45758.0
32	48864.0
33	48864.0
34	52034.0
35	55204.0
36	57667.0
37	60130.0
38	62487.5
39	64845.0
40	64845.0
41	66094.0
42	67343.0
43	64287.5
44	61232.0
45	60782.5
46	60333.0
47	60333.0
48	58605.5
49	56878.0
50	55345.0
51	53812.0
52	55774.0
53	57736.0
54	57736.0
55	49300.5
56	40865.0
57	37930.5
58	34996.0
59	29372.0
60	23748.0
61	23748.0
62	19915.0
63	16082.0
64	12997.5
65	9913.0
66	8135.5
67	6358.0
68	6358.0
69	5066.5
70	3775.0
71	3040.0
72	2305.0
73	1802.5
74	1300.0
75	1300.0
76	977.5
77	655.0
78	494.0
79	333.0
80	233.0
81	133.0
82	133.0
83	99.5
84	66.0
85	51.0
86	36.0
87	28.0
88	20.0
89	20.0
90	15.0
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	869280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.189727611901354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4424373854123	46.5767081012106
2	11.559690492400424	13.221911010765321
3	3.0181571529683096	5.178227564045079
4	1.207867424208995	2.763104359272053
5	0.6052285579300116	1.7306428185480611
6	0.377925209484871	1.2968063872866316
7	0.2727899415226033	1.0920547715660927
8	0.17903254356124912	0.819105791994696
9	0.13917702979103502	0.7163546781224495
>10	0.9971297673913526	11.246701103928753
>50	0.12537963625819448	4.97003968524045
>100	0.06994409143123301	6.678064526983224
>500	0.003628223750424445	1.3117276775180784
>1k	0.0016125438890775311	2.3985515235186448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4042	0.46498251426467885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3957	0.4552043070127002	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	3335	0.3836508374746917	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3176	0.36535983802687283	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2029	0.23341155899134916	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	1966	0.22616418185164733	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.1458678446530462	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1034	0.11894901527701085	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	968	0.11135652494018039	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	889	0.10226854408245906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.150377323762194E-4	0.0
8	0.0	0.0	0.0	2.300754647524388E-4	0.0
9	0.0	0.0	0.0	2.300754647524388E-4	0.0
10	0.0	0.0	0.0	2.300754647524388E-4	0.0
11	0.0	0.0	0.0	3.451131971286582E-4	0.0
12	0.0	0.0	0.0	3.451131971286582E-4	0.0
13	0.0	0.0	0.0	3.451131971286582E-4	0.0
14	0.0	0.0	0.0	3.451131971286582E-4	0.0
15	0.0	0.0	0.0	3.451131971286582E-4	0.0
16	0.0	0.0	0.0	3.451131971286582E-4	0.0
17	0.0	0.0	0.0	3.451131971286582E-4	0.0
18	0.0	0.0	0.0	5.75188661881097E-4	0.0
19	0.0	0.0	0.0	5.75188661881097E-4	0.0
20	0.0	0.0	0.0	5.75188661881097E-4	0.0
21	0.0	0.0	0.0	6.902263942573164E-4	1.150377323762194E-4
22	0.0	0.0	0.0	0.001150377323762194	1.150377323762194E-4
23	0.0	0.0	0.0	0.0013804527885146328	1.150377323762194E-4
24	0.0	0.0	0.0	0.0021857169151481685	1.150377323762194E-4
25	0.0	0.0	0.0	0.0021857169151481685	1.150377323762194E-4
26	0.0	0.0	0.0	0.0026458678446530464	1.150377323762194E-4
27	0.0	0.0	0.0	0.003106018774157924	1.150377323762194E-4
28	0.0	0.0	0.0	0.008512792195840236	1.150377323762194E-4
29	0.0	0.0	0.0	0.01725565985643291	1.150377323762194E-4
30	0.0	0.0	0.0	0.03267071599484631	1.150377323762194E-4
31	0.0	0.0	0.0	0.07696024295969078	1.150377323762194E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	115	0.0	32.173912	1
GGTATCA	545	0.0	27.155966	1
ATTACGG	140	0.0	26.42857	2
TCGACTA	35	8.8676496E-4	26.42857	27
GCCCGAT	35	8.8676496E-4	26.42857	12
GCAACGC	50	9.087764E-6	25.899998	3
GTATCAA	1795	0.0	25.559887	1
TTACGGG	155	0.0	25.064516	3
CTTATAC	585	0.0	24.666668	1
TATAACG	45	1.3228496E-4	24.666666	2
CGTCTTC	135	0.0	23.296297	37
ACCGATG	40	0.0019308105	23.125	27
CGCCCGA	40	0.0019308105	23.125	11
CCGTCTT	325	0.0	22.76923	37
GCCGTCT	335	0.0	22.089552	36
TTATACA	680	0.0	21.764706	2
TATACAC	710	0.0	21.105635	3
CGAACGC	45	0.003825069	20.555555	30
ACTACGC	45	0.003825069	20.555555	30
ATGACCG	45	0.003825069	20.555555	5
>>END_MODULE