FastQCFastQC Report
Fri 10 Feb 2017
ERR1630979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796720
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT124461.5621548348227734No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG111521.399738929611407No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT84361.0588412491213979TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC57370.7200773169996988No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCA29190.3663771462998293No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC28940.3632392810523145RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC25440.31930916758710715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23970.3008585199317201No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT21510.2699819258961743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.2502761321417813No Hit
GGTATAGAGACTGAAGAGATACTGGGGGCTTTAGCTATTGGCA17610.22103122803494327No Hit
CTTTATTGGTATAGAGACTGAAGAGATACTGGGGGCTTTAGCT14550.182623757405362No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC14370.18036449442715133No Hit
CTCTACCACCATGAATCCACTCCTGATCCTTACCTTTGTGGCA13720.17220604478361282No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG13180.16542825584898083No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA12650.1587759815242494No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA12080.15162164875991566No Hit
AATGAAGACCGTGAGACCTGGGTTGATGCAGATCTCTATTGCC11580.14534591826488602No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA10810.13568129330254042No Hit
ATTTAAGGTTCCAAAGACTGGGGTAGGTAAAACTATTGAAGAT10440.1310372527362185No Hit
GTTCCAAAGACTGGGGTAGGTAAAACTATTGAAGATTAACAAG10350.12990762124711316No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA9480.1189878501857616No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG9430.11836027713625866No Hit
TCCAAAGACTGGGGTAGGTAAAACTATTGAAGATTAACAAGGC9200.11547344110854503No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT8670.10882116678381364No Hit
AGGTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAA8500.10668741841550355No Hit
GAAGAGATACTGGGGGCTTTAGCTATTGGCAGCTATGGTGTTC8360.10493021387689527No Hit
GGATTAACACTGCTTGGGGCTCCAATGCCCCAGGACTTGTAGG8300.10417712621749171No Hit
CCTTAATCAGTGAGGCCACAAAGGCACCCTCGGCCTGGGTGAG8150.10229440706898284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8050.10103926096997691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG750.032.0666661
CTTATAC15850.031.9810711
TTATACA16900.029.9940832
TATACAC17450.029.6848143
AGCCTTA652.9867806E-928.4615387
CGTCTTC4450.027.0224737
GCGGTCG358.866996E-426.42857237
TATTCGC358.866996E-426.42857227
CGCTTAA358.866996E-426.42857231
GACAGCG1550.026.25806437
CCGTCTT12300.025.11788737
GTATCAA13500.025.0777781
GCCGTCT13200.023.40530436
ATTCGCT400.001930669523.12500228
CGCGTTG400.001930669523.12500234
GGCCGTA1550.022.677427
CTAAACA903.8235157E-922.611114
GCCGTAT1600.021.9687528
TCTAGAC4850.021.7422683
CCGAACA603.724439E-521.58333421
Produced by FastQC (version 0.11.5)