##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630979.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	796720
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07068104227332	31.0	31.0	34.0	28.0	34.0
2	31.04796540817351	31.0	31.0	34.0	28.0	34.0
3	30.463627121196907	31.0	30.0	34.0	26.0	34.0
4	34.70465910231951	35.0	35.0	37.0	32.0	37.0
5	34.82544181142685	35.0	35.0	37.0	32.0	37.0
6	35.054169595340895	36.0	35.0	37.0	32.0	37.0
7	35.077239180640625	36.0	35.0	37.0	32.0	37.0
8	35.12206797871272	37.0	35.0	37.0	32.0	37.0
9	36.83955467416407	39.0	37.0	39.0	33.0	39.0
10	36.61316899287077	38.0	35.0	39.0	32.0	39.0
11	36.70256426348027	38.0	35.0	39.0	32.0	39.0
12	36.61950873581685	38.0	35.0	39.0	32.0	39.0
13	36.691055828898484	38.0	35.0	39.0	32.0	39.0
14	37.72054297620243	39.0	37.0	40.0	33.0	41.0
15	37.68309192690029	39.0	37.0	40.0	32.0	41.0
16	37.73162842654885	39.0	37.0	40.0	33.0	41.0
17	37.6486092981223	39.0	37.0	40.0	32.0	41.0
18	37.74665754593835	39.0	37.0	40.0	33.0	41.0
19	37.73382744251431	39.0	37.0	40.0	32.0	41.0
20	37.756725072798474	39.0	37.0	40.0	32.0	41.0
21	37.70493272416909	39.0	37.0	40.0	32.0	41.0
22	37.6203270910734	39.0	37.0	40.0	32.0	41.0
23	37.63952580580379	39.0	37.0	40.0	32.0	41.0
24	37.54474093784516	39.0	37.0	40.0	32.0	41.0
25	37.53334797670449	39.0	37.0	40.0	32.0	41.0
26	37.38448890450849	39.0	36.0	40.0	32.0	41.0
27	37.28378225725474	39.0	36.0	40.0	32.0	41.0
28	37.293306305854	39.0	36.0	40.0	32.0	41.0
29	37.22510794256451	39.0	36.0	40.0	31.0	41.0
30	37.17658022893865	39.0	36.0	40.0	31.0	41.0
31	37.14070564313686	39.0	36.0	40.0	31.0	41.0
32	37.01478938648459	39.0	36.0	40.0	31.0	41.0
33	36.964901094487395	39.0	36.0	40.0	31.0	41.0
34	36.85121121598554	39.0	36.0	40.0	30.0	41.0
35	36.85634476353047	39.0	36.0	40.0	30.0	41.0
36	36.84750476955518	39.0	36.0	40.0	30.0	41.0
37	36.75524525554775	39.0	35.0	40.0	30.0	41.0
38	36.70671001104529	39.0	35.0	40.0	30.0	41.0
39	36.6489908625364	39.0	35.0	40.0	30.0	41.0
40	36.55701375640125	38.0	35.0	40.0	30.0	41.0
41	36.501625414198216	38.0	35.0	40.0	30.0	41.0
42	36.44800055226428	38.0	35.0	40.0	30.0	41.0
43	35.696190631589516	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	9.0
10	7.0
11	19.0
12	5.0
13	8.0
14	9.0
15	6.0
16	10.0
17	16.0
18	22.0
19	48.0
20	90.0
21	230.0
22	435.0
23	853.0
24	1475.0
25	2488.0
26	3908.0
27	6001.0
28	8856.0
29	12400.0
30	16975.0
31	22612.0
32	28888.0
33	37567.0
34	48900.0
35	65036.0
36	91122.0
37	132612.0
38	199023.0
39	117087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63786524751481	20.977633296515712	14.110980018074104	24.273521437895372
2	15.773546540817351	21.982628777989756	38.84137463600763	23.40245004518526
3	16.764484385982527	25.68857314991465	34.33904006426348	23.20790239983934
4	13.45955919269003	18.15091876694447	36.04842353649965	32.34109850386585
5	13.551310372527361	37.149814238377346	34.745205341901794	14.553670047193492
6	30.159278039963848	38.86045285671253	15.882744251430866	15.09752485189276
7	27.199643538507885	32.00547243699167	20.97888844261472	19.81599558188573
8	25.645270609498944	34.33954212270308	21.435008534993475	18.580178732804498
9	25.022467115172205	13.891329450748065	18.86246109047093	42.22374234360879
10	14.285194296616128	28.91542825584898	32.50263580680791	24.296741640726978
11	32.54480871573451	22.354905110954913	24.30966964554674	20.79061652776383
12	22.695551762225122	25.019454764534593	28.795687318003814	23.48930615523647
13	30.51297821066372	20.1063108745858	26.166407269806207	23.21430364494427
14	23.555703383873883	21.179586303845767	25.191786323928106	30.072923988352247
15	24.61015162164876	28.230746058841248	22.43423034441209	24.7248719750979
16	26.47090571342504	26.39635003514409	22.191987147303944	24.94075710412692
17	21.851466010643637	29.719725876091978	24.386359072196004	24.04244904106838
18	25.88136359072196	24.547394316698465	24.79478361281253	24.776458479767044
19	25.085349934732402	26.61851089466814	25.154006426348026	23.14213274425143
20	23.94492418917562	25.444572748267895	25.871698965759617	24.73880409679687
21	24.794407068982828	25.09777588111256	25.772542423938145	24.335274625966463
22	22.146927402349633	28.161587508786024	26.422080530173712	23.26940455869063
23	23.835600963952206	24.437694547645346	24.97012752284366	26.756576965558793
24	23.62498744853901	28.010969976905315	25.51134652073501	22.852696053820665
25	25.60987548950698	26.623782508283966	25.04003916055829	22.72630284165077
26	27.07287378250828	26.384049603373832	23.83233758409479	22.710739030023095
27	23.628752886836025	26.722060447836128	27.39168089165579	22.257505773672058
28	22.443518425544735	24.26762225123004	26.06812933025404	27.220729992971183
29	22.176423335676272	26.57294909127422	26.59616929410583	24.654458278943668
30	22.829099307159353	24.758133346721557	28.87890350436791	23.533863841751177
31	24.828044984436186	25.844713324630987	24.998117280851492	24.329124410081334
32	25.572723164976402	24.96673862837634	27.615724470328345	21.84481373631891
33	24.510995079827293	24.419243899989958	27.041495130033137	24.028265890149612
34	23.389647554975397	27.385405161160758	25.121247113163975	24.103700170699867
35	24.085500552264282	24.734160056230543	29.300883622853704	21.87945576865147
36	26.00210864544633	23.619966864142985	25.87282859724872	24.50509589316196
37	23.78627372226127	25.817225625062758	27.357415403152928	23.039085249523044
38	23.4113615824882	23.51453459182649	28.79066673360779	24.283437092077516
39	25.9326990661713	24.320338387388293	26.377146299829302	23.369816246611105
40	23.503238276935434	24.301385681293304	29.375941359574252	22.819434682197006
41	25.62895371021187	24.045963450145596	25.19479867456572	25.130284165076816
42	24.499949794156038	23.32375238477759	27.593006325936337	24.583291495130034
43	24.03240787227633	24.20963450145597	27.474897078019882	24.283060548247818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	14.0
2	17.0
3	73.5
4	130.0
5	130.0
6	178.5
7	227.0
8	231.0
9	235.0
10	371.0
11	507.0
12	507.0
13	729.5
14	952.0
15	2081.5
16	3211.0
17	3719.0
18	4227.0
19	4227.0
20	4210.0
21	4193.0
22	4915.0
23	5637.0
24	7251.5
25	8866.0
26	8866.0
27	10589.0
28	12312.0
29	14651.5
30	16991.0
31	19314.5
32	21638.0
33	21638.0
34	25466.5
35	29295.0
36	33683.5
37	38072.0
38	40928.0
39	43784.0
40	43784.0
41	47057.5
42	50331.0
43	52391.0
44	54451.0
45	57025.5
46	59600.0
47	59600.0
48	61474.0
49	63348.0
50	76751.0
51	90154.0
52	75080.0
53	60006.0
54	60006.0
55	65398.0
56	70790.0
57	60869.0
58	50948.0
59	42221.5
60	33495.0
61	33495.0
62	29692.5
63	25890.0
64	22879.0
65	19868.0
66	17117.0
67	14366.0
68	14366.0
69	10490.5
70	6615.0
71	5370.0
72	4125.0
73	2846.5
74	1568.0
75	1568.0
76	1010.5
77	453.0
78	317.0
79	181.0
80	138.0
81	95.0
82	95.0
83	81.5
84	68.0
85	54.0
86	40.0
87	29.0
88	18.0
89	18.0
90	10.5
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	796720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.46552325543631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.38717906786827	29.313605477382737
2	9.582991603145533	6.988976063223085
3	2.853596854528757	3.1217370738137484
4	1.4562743528131865	2.1241522511521884
5	0.9072266848938354	1.6541247887974273
6	0.643230988015501	1.4073452731257914
7	0.5005979608063058	1.2778196606984435
8	0.39113935810155803	1.1410481087175037
9	0.3062377602209735	1.0050408150327572
>10	2.416347745942461	17.93732116367315
>50	0.31739334537123354	8.12240324918467
>100	0.21901183526926354	14.56931431337921
>500	0.011472048514104277	2.8685052883357955
>1k	0.005909843173932507	3.6804540408455524
>5k	6.952756675214713E-4	1.796682227840871
>10k+	6.952756675214713E-4	2.99147020479707
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	12446	1.5621548348227734	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	11152	1.399738929611407	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	8436	1.0588412491213979	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	5737	0.7200773169996988	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCA	2919	0.3663771462998293	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	2894	0.3632392810523145	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	2544	0.31930916758710715	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2397	0.3008585199317201	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	2151	0.2699819258961743	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1994	0.2502761321417813	No Hit
GGTATAGAGACTGAAGAGATACTGGGGGCTTTAGCTATTGGCA	1761	0.22103122803494327	No Hit
CTTTATTGGTATAGAGACTGAAGAGATACTGGGGGCTTTAGCT	1455	0.182623757405362	No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC	1437	0.18036449442715133	No Hit
CTCTACCACCATGAATCCACTCCTGATCCTTACCTTTGTGGCA	1372	0.17220604478361282	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1318	0.16542825584898083	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	1265	0.1587759815242494	No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA	1208	0.15162164875991566	No Hit
AATGAAGACCGTGAGACCTGGGTTGATGCAGATCTCTATTGCC	1158	0.14534591826488602	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1081	0.13568129330254042	No Hit
ATTTAAGGTTCCAAAGACTGGGGTAGGTAAAACTATTGAAGAT	1044	0.1310372527362185	No Hit
GTTCCAAAGACTGGGGTAGGTAAAACTATTGAAGATTAACAAG	1035	0.12990762124711316	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA	948	0.1189878501857616	No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG	943	0.11836027713625866	No Hit
TCCAAAGACTGGGGTAGGTAAAACTATTGAAGATTAACAAGGC	920	0.11547344110854503	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	867	0.10882116678381364	No Hit
AGGTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAA	850	0.10668741841550355	No Hit
GAAGAGATACTGGGGGCTTTAGCTATTGGCAGCTATGGTGTTC	836	0.10493021387689527	No Hit
GGATTAACACTGCTTGGGGCTCCAATGCCCCAGGACTTGTAGG	830	0.10417712621749171	No Hit
CCTTAATCAGTGAGGCCACAAAGGCACCCTCGGCCTGGGTGAG	815	0.10229440706898284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	805	0.10103926096997691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2551460990059242E-4	0.0
5	0.0	0.0	0.0	1.2551460990059242E-4	0.0
6	0.0	0.0	0.0	1.2551460990059242E-4	0.0
7	0.0	0.0	0.0	2.5102921980118485E-4	0.0
8	0.0	0.0	0.0	2.5102921980118485E-4	0.0
9	0.0	0.0	0.0	2.5102921980118485E-4	0.0
10	0.0	0.0	0.0	2.5102921980118485E-4	0.0
11	0.0	0.0	0.0	2.5102921980118485E-4	0.0
12	0.0	0.0	0.0	5.020584396023697E-4	0.0
13	0.0	0.0	0.0	6.275730495029621E-4	0.0
14	0.0	0.0	0.0	6.275730495029621E-4	0.0
15	0.0	0.0	0.0	6.275730495029621E-4	0.0
16	0.0	0.0	0.0	6.275730495029621E-4	0.0
17	0.0	0.0	0.0	6.275730495029621E-4	0.0
18	0.0	0.0	0.0	6.275730495029621E-4	0.0
19	0.0	0.0	0.0	7.530876594035546E-4	0.0
20	0.0	0.0	0.0	8.78602269304147E-4	0.0
21	0.0	0.0	0.0	0.0015061753188071092	0.0
22	0.0	0.0	0.0	0.001757204538608294	0.0
23	0.0	0.0	0.0	0.0022592629782106637	0.0
24	0.0	0.0	0.0	0.0025102921980118486	0.0
25	0.0	0.0	0.0	0.0025102921980118486	0.0
26	0.0	0.0	0.0	0.0025102921980118486	0.0
27	0.0	0.0	0.0	0.002886836027713626	0.0
28	0.0	0.0	0.0	0.006401245104930214	0.0
29	0.0	0.0	0.0	0.012049402550456874	0.0
30	0.0	0.0	0.0	0.023973290491013155	0.0
31	0.0	0.0	0.0	0.04957827091073401	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	75	0.0	32.066666	1
CTTATAC	1585	0.0	31.981071	1
TTATACA	1690	0.0	29.994083	2
TATACAC	1745	0.0	29.684814	3
AGCCTTA	65	2.9867806E-9	28.461538	7
CGTCTTC	445	0.0	27.02247	37
GCGGTCG	35	8.866996E-4	26.428572	37
TATTCGC	35	8.866996E-4	26.428572	27
CGCTTAA	35	8.866996E-4	26.428572	31
GACAGCG	155	0.0	26.258064	37
CCGTCTT	1230	0.0	25.117887	37
GTATCAA	1350	0.0	25.077778	1
GCCGTCT	1320	0.0	23.405304	36
ATTCGCT	40	0.0019306695	23.125002	28
CGCGTTG	40	0.0019306695	23.125002	34
GGCCGTA	155	0.0	22.67742	7
CTAAACA	90	3.8235157E-9	22.61111	4
GCCGTAT	160	0.0	21.968752	8
TCTAGAC	485	0.0	21.742268	3
CCGAACA	60	3.724439E-5	21.583334	21
>>END_MODULE