FastQCFastQC Report
Fri 10 Feb 2017
ERR1630978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623205
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125452.0129812822426008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122581.966929020145859No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT86971.395527956290466No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG72121.1572436036296243No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA69281.1116727240635105No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT48840.7836907598623245RNA PCR Primer, Index 29 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA46730.7498335218748245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42770.6862910278319333No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC40010.6420038350141607No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT37560.6026909283462103No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA31910.5120305517446105No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT29270.4696688890493498No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG28890.4635713769947289No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT28570.4584366300013639No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC27650.4436742323954397No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC25490.4090146901902263No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG23570.37820620823003664No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG21360.34274436180711No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC21160.3395351449362569RNA PCR Primer, Index 29 (95% over 21bp)
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT19200.30808481960189665No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG19130.3069615936970981No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG18170.2915573527170032No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG17550.28160878041735865No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG16990.27262297317897No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16590.26620453943726385No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC16590.26620453943726385RNA PCR Primer, Index 29 (95% over 24bp)
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG16440.263797626784124No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG16110.25850241894721643No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG15340.246146933994432No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA15250.2447027864025481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.24390048218483484No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC15040.24133310868815236No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG14890.23892619603501256No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14880.23876573519146987No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT13980.22432425927263097No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA13690.219670894809894No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13450.21581983456487028No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13440.21565937372132765No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT13310.21357338275527316No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG13030.2090804791360788No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC12920.20731540985710964No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG12780.20506895804751246No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.1919111688770148No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA11510.18469043091759535No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG10840.17393955440023748No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10570.1696071116245858No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA10420.167200198971446No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG10310.16543512969247678No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10230.16415144294413556No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA9910.15901669595077061No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC9800.15725162667180143No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG9540.1530796447396924No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG9430.1513145754607232No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9420.15115411461718053No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9390.15067273208655257No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG9360.1501913495559246No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC9250.14842628027695542No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG9240.14826581943341274No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9180.14730305437215685No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8880.14248922906587722No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA8860.14216830737879188No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT8860.14216830737879188No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG8710.13976139472565208No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT8600.1379963254466829No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG8410.13494756941937244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC8400.1347871085758298No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8230.13205927423560465No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8210.13173835254851934No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC8140.13061512664372077No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8140.13061512664372077No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8050.12917097905183686No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8000.1283686748341236No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG7970.12788729230349563No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG7760.12451761458909989No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC7670.123073466997216No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA7630.12243162362304538No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA7630.12243162362304538No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA7540.1209874760311615No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT7520.12066655434407618No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG7490.12018517181344823No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7390.11858056337802167No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA7360.11809918084739371No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC7330.11761779831676576No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG7280.1168154940990525No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA7070.11344581638465673No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA6880.11039706035734631No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6710.10766922601712119No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6690.10734830433003587No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA6620.10622507842523728No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6550.1051018525204387No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6550.1051018525204387No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA6450.10349724408501215No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA6420.1030158615543842No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG6400.10269493986729888No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG6400.10269493986729888No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA6390.10253447902375623No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACT6310.10125079227541499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT200.001841289937.037
ACAGACA200.001841289937.017
CTTTATA200.001841289937.01
GATTACG1350.037.01
GACTACT200.001841289937.01
CATTAGC251.2329775E-436.9999961
TTACGGG1450.034.4482773
ACTCAGA603.45608E-1133.9166681
GTTTGTG554.984031E-1033.6363641
ATTACGG1500.033.32
TAGCAAC451.07862434E-732.888891
CTTATAC12800.032.6640621
TATACAC13100.032.057253
TTGTGTT352.3865663E-531.7142873
TTATACA13400.031.4776122
GATTAGG303.5976036E-430.8333341
ACTGGGA303.5976036E-430.8333341
ATTACTT551.8528226E-830.2727282
CGTCTTC2200.030.27272837
AGCAACG502.7174065E-729.5999982
Produced by FastQC (version 0.11.5)