FastQCFastQC Report
Fri 10 Feb 2017
ERR1630977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409370
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT269736.5889049026553No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG213555.2165522632337495No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT168304.111195251239709TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC111082.7134377213767498No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC70791.7292424945648193RNA PCR Primer, Index 8 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57181.3967804186921366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57101.3948261963504898No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC53501.3068861909763783No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA42451.0369592300363975No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG25960.6341451498644258No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA24810.6060532037032513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22060.5388768107091385No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT21530.5259300876957276No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA21470.5244644209394924No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19000.4641278061411437No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17410.42528763710091116No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17400.42504335930820525No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC17320.42308913696655837No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT17000.41527224759997067No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16180.39524146859808973No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC15500.37863057869409095No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG14640.3576226885213865No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT12550.3065686298458607No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11650.2845836285023328No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11150.2723697388670396No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10950.2674841830129223No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10730.2621100715733933No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10520.2569802379265701No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10070.24598773725480616No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10060.2457434594621003No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9930.24256784815692403No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9800.2393922368517478No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9320.22766690280186627No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8990.21960573564257274No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8770.2142316242030437No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG8750.21374306861763198No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8130.19859784546986833No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7860.19200234506680997No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7810.19078095610328064No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7700.18809390038351614No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7200.17588001074822288No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7060.17246012165034078No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA7050.1722158438576349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.17099445489410559No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6230.15218506485575398No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6210.15169650927034223No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC6160.1504751203068129No Hit
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT6160.1504751203068129TruSeq Adapter, Index 8 (95% over 21bp)
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6010.14681095341622494No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT5930.144856731074578TruSeq Adapter, Index 8 (95% over 24bp)
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5910.14436817548916628No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5890.14387961990375456No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5840.14265823094022523No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5640.1377726750861079No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5500.1343527859882258No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG5450.13313139702469648No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5430.13264284143928473No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5360.1309328968903437No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5350.13068861909763782No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5310.12971150792681435No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5250.1282458411705792No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA5250.1282458411705792No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5150.12580306324352053No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5080.12409311869457948No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4930.1204289518039915No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4840.11823045166963872No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4770.11652050712069766No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4760.11627622932799178No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4710.11505484036446247No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4700.1148105625717566No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4620.1128563402301097No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4530.1106578400957569No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4520.11041356230305102No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4520.11041356230305102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.10968072892493343No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4330.10577228424163959No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4290.10479517307081614No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4280.10455089527811026No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4270.1043066174854044No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4230.10332950631458093No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4160.10161956176563988No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA4100.1001538950094047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG200.001840551837.01
TCGCCGA403.82297E-837.01
AGCAACG251.2322376E-437.02
ATTACGG403.82297E-837.02
TTACTTT355.619495E-737.03
CGTTACT251.2322376E-437.01
TCAGAGT355.619495E-737.03
GTATACA251.2322376E-437.02
GATTACG403.82297E-837.01
CTTATAC31700.035.541011
GACAGAC700.034.35714316
ACAGACA700.034.35714317
CAGACAC700.034.35714318
TTATACA33050.034.2012062
TTTACAC554.984031E-1033.6363642
CGTCTTC8550.032.8888937
TTACGGG451.0770782E-732.888893
TATACAC34700.032.84153
AGGTAGC352.3845794E-531.7142877
TAGCGAA352.3845794E-531.71428710
Produced by FastQC (version 0.11.5)