FastQCFastQC Report
Fri 10 Feb 2017
ERR1630976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320016
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT80050.6064320432479606No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG55890.42340395873989406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46670.35355632052944813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.31090532235972895No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT39910.3023448200627871TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC30750.23295172179731155No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30370.23007296881249922No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC18510.14022557302335728No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18000.1363619834911092No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17910.13568017357365364No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17260.1307559908364747No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC16420.1243924316068896No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15240.11545314602247245No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT14710.11143804317523423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14710.11143804317523423No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC14600.11060471994278856No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14440.1093926134228676No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14140.10711991369801578No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC13940.10560478054811456RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC13920.10545326723312444No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT13530.10249875759081707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG900.030.8333341
CTTATAC11350.028.524231
GGTATCA6250.028.1200031
GTATCAA22700.026.5682811
TATACAC13400.024.8507463
CCGTCTT7400.024.037
TTATACA13850.023.9097482
GCCGTCT7600.023.36842236
TTACGGG1200.023.1249983
CGTCTTC3200.022.54687737
TGCCGTC8800.020.18181835
ATGCCGT9450.018.79365234
TAGGTAC1103.8529834E-818.53
GTTCGCC1601.8189894E-1218.524
GGTTCGC1601.8189894E-1218.523
TACGTTA701.21937126E-418.519
CGGTTCG1705.456968E-1217.41176422
TATGCCG10400.017.07692333
AACGGCC1301.3935278E-817.07692337
ATTACGG1655.4569682E-1116.8181822
Produced by FastQC (version 0.11.5)