FastQCFastQC Report
Fri 10 Feb 2017
ERR1630975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992553
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT219462.2110658070652147No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG164451.6568384761317532No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT122811.2373142794389822Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC87310.8796507592037907No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC45410.45750705503887446Illumina PCR Primer Index 10 (95% over 22bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG38260.3854705995548853No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC37550.3783173291501814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28630.2884480727981276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.26910401761921027No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG21410.21570636530240705No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG18560.1869925333962015No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA18190.1832647727627643No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17300.17429799718503697No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA15400.15515544258089994No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC14890.15001717792400002No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG14700.14810292246358633No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT13020.1311768741820336No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT11480.11566132992394361No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC10770.10850805951923978No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG10520.10598930233448492No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10420.10498179946058295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25900.034.2142871
TTATACA27700.031.7238252
TATACAC29450.030.2784393
GATTACG801.8189894E-1230.0625021
CGTCTTC6250.028.41637
ATTACGG905.456968E-1226.7222212
GTATCAA14050.025.8078291
CCGTCTT18550.025.73045737
GGTATCA4750.025.7052631
GCCGTCT18850.025.32095536
TTACGGG951.0913936E-1125.315793
TGCCGTC22500.021.21333335
ATGCCGT23550.020.1889634
TATGCCG25700.018.64396933
TAGACTG701.2189845E-418.55
GTCCTAA1205.171387E-918.51
TACACAT49700.018.3883295
ATACACA49800.018.239964
ACGGTAC1552.0008883E-1117.9032273
TCGCTGC2400.016.187525
Produced by FastQC (version 0.11.5)