##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630975.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992553
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413785460323027	31.0	31.0	34.0	28.0	34.0
2	31.403010217086646	31.0	31.0	34.0	28.0	34.0
3	30.870065376861486	31.0	31.0	34.0	27.0	34.0
4	35.04299619264664	37.0	35.0	37.0	32.0	37.0
5	35.23796008878115	37.0	35.0	37.0	33.0	37.0
6	35.42735652403449	37.0	35.0	37.0	33.0	37.0
7	35.42981281604106	37.0	35.0	37.0	33.0	37.0
8	35.457968491355125	37.0	35.0	37.0	33.0	37.0
9	37.23556021693552	39.0	37.0	39.0	34.0	39.0
10	37.047113856892274	39.0	37.0	39.0	33.0	39.0
11	37.12154413920466	39.0	37.0	39.0	33.0	39.0
12	37.033121656979525	39.0	37.0	39.0	33.0	39.0
13	37.121505854095446	39.0	37.0	39.0	33.0	39.0
14	38.23796714130127	40.0	38.0	41.0	33.0	41.0
15	38.24718780760322	40.0	38.0	41.0	33.0	41.0
16	38.24782354191665	40.0	38.0	41.0	33.0	41.0
17	38.18817534177016	40.0	38.0	41.0	33.0	41.0
18	38.230554942657974	40.0	38.0	41.0	33.0	41.0
19	38.253401077826574	40.0	38.0	41.0	34.0	41.0
20	38.26946067363657	40.0	38.0	41.0	34.0	41.0
21	38.230976078859264	40.0	38.0	41.0	34.0	41.0
22	38.15509902241996	40.0	38.0	41.0	33.0	41.0
23	38.15186594569761	40.0	38.0	41.0	33.0	41.0
24	38.10108175583571	40.0	37.0	41.0	33.0	41.0
25	38.07649364819813	40.0	37.0	41.0	33.0	41.0
26	37.945077995834986	40.0	37.0	41.0	33.0	41.0
27	37.88360117797236	40.0	37.0	41.0	33.0	41.0
28	37.87742921536684	40.0	37.0	41.0	33.0	41.0
29	37.795860775192864	40.0	37.0	41.0	33.0	41.0
30	37.729641641302784	40.0	37.0	41.0	33.0	41.0
31	37.69976515108009	40.0	37.0	41.0	33.0	41.0
32	37.61243681697602	40.0	37.0	41.0	32.0	41.0
33	37.53090565440838	39.0	37.0	41.0	32.0	41.0
34	37.46253348687677	39.0	36.0	41.0	32.0	41.0
35	37.47770245014624	39.0	36.0	41.0	32.0	41.0
36	37.4060075381365	39.0	36.0	41.0	32.0	41.0
37	37.33440934640266	39.0	36.0	41.0	31.0	41.0
38	37.32218531403361	39.0	36.0	41.0	31.0	41.0
39	37.21477946265842	39.0	36.0	41.0	31.0	41.0
40	37.11355867142611	39.0	36.0	41.0	31.0	41.0
41	37.06969501880504	39.0	36.0	41.0	31.0	41.0
42	36.998806109094424	39.0	36.0	40.0	31.0	41.0
43	36.28205445956035	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	19.0
11	17.0
12	21.0
13	11.0
14	5.0
15	9.0
16	9.0
17	17.0
18	21.0
19	45.0
20	100.0
21	201.0
22	388.0
23	747.0
24	1391.0
25	2377.0
26	3775.0
27	5884.0
28	8867.0
29	12349.0
30	16899.0
31	22856.0
32	29579.0
33	38588.0
34	50781.0
35	67883.0
36	95445.0
37	140368.0
38	238910.0
39	254980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.43013622446358	21.599954863871247	14.478219299120552	24.49168961254462
2	14.272890213419334	21.8686558803409	40.706440865122566	23.1520130411172
3	15.072948245584872	27.458684825898466	33.943275573193574	23.52509135532309
4	11.61217587373168	19.414378879515752	36.654365056576324	32.319080190176244
5	13.305485953898682	37.23791072114033	35.16860056843312	14.288002756527863
6	28.665572518545606	39.21362385686205	17.331568188298256	14.789235436294081
7	26.259252654518196	32.27988832838145	21.295386745090692	20.165472272009655
8	25.593998506880737	34.36128851557549	21.980287198769236	18.064425778774535
9	24.048589848602543	14.433989922956256	19.065984385720462	42.45143584272074
10	14.74188280122069	28.700532868270006	32.815073854998175	23.742510475511132
11	32.25631276113215	22.66015013807827	24.68230915628687	20.40122794450271
12	21.21649927006417	25.2358312352086	28.880170630686724	24.66749886404051
13	29.59630367345623	20.20365663093054	26.645025504935255	23.555014190677976
14	22.246670958628908	21.841554053032937	25.652332923279666	30.25944206505849
15	24.16052341789305	28.157690319811636	22.41109542764971	25.270690834645603
16	26.160315872301027	26.759780082272684	22.462679574793487	24.617224470632802
17	22.333013954922308	29.805965021515224	23.867541582162364	23.993479441400105
18	24.60070142350081	24.919878333952948	25.417383253085728	25.062036989460513
19	24.861745418128805	27.713079301558707	24.64271429334252	22.782460986969966
20	23.556928446138393	25.542313609449568	24.729258790210697	26.17149915420134
21	24.800791494257737	25.230189219114745	25.55188488675164	24.417134399875877
22	22.963912254559705	29.070689424141584	24.77792117902016	23.18747714227855
23	24.535918988708914	24.614201962011098	25.50664800771344	25.343231041566543
24	23.786538351100646	27.66300640872578	25.07281727021126	23.47763796996231
25	22.38701610896345	27.660689152115808	26.347912907421566	23.604381831499175
26	25.170343548404972	27.834987149300844	24.798977989084715	22.19569131320947
27	22.062801684141803	28.244839318404157	24.832024083348696	24.86033491410534
28	22.61985002312219	24.880182720721212	28.186605652292624	24.313361603863974
29	22.089802761162375	27.774839227728897	25.268978079759975	24.866379931348753
30	23.70130360796854	24.985366020756576	27.036138120583992	24.277192250690895
31	22.848553175497933	24.582566371770575	27.18283053902411	25.38604991370738
32	22.741757870864326	25.416678001074	27.462916337968856	24.37864779009282
33	24.33734017226284	26.081932148711456	27.38483486524145	22.19589281378425
34	22.606248734324513	27.090744776349474	25.47803492609463	24.824971563231387
35	22.358604527919415	25.167824791220216	30.810143135933295	21.66342754492707
36	23.180928373598185	27.247310722953838	24.313462354151365	25.258298549296608
37	23.215787973035194	26.49923983908164	27.22454115800365	23.060431029879513
38	22.60866674122188	23.895550162056836	27.70330652368186	25.792476573039423
39	24.901844032510105	24.670924373811776	26.126866776887482	24.30036481679064
40	22.4111961779371	24.11921580006307	30.747174206314426	22.72241381568541
41	23.46826819323502	25.419297508546144	25.32015922575419	25.792275072464644
42	23.79752013242618	25.157850512768587	28.262369868410048	22.782259486395184
43	24.607854693905516	23.465447185188097	28.21400973046276	23.712688390443635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	9.0
2	17.0
3	91.5
4	166.0
5	166.0
6	205.0
7	244.0
8	244.0
9	244.0
10	384.0
11	524.0
12	524.0
13	818.5
14	1113.0
15	2700.5
16	4288.0
17	4841.0
18	5394.0
19	5394.0
20	5419.5
21	5445.0
22	6914.5
23	8384.0
24	10833.5
25	13283.0
26	13283.0
27	15891.0
28	18499.0
29	22141.0
30	25783.0
31	30207.0
32	34631.0
33	34631.0
34	43236.0
35	51841.0
36	54276.5
37	56712.0
38	57406.0
39	58100.0
40	58100.0
41	58482.0
42	58864.0
43	61386.5
44	63909.0
45	64657.5
46	65406.0
47	65406.0
48	66898.0
49	68390.0
50	86626.0
51	104862.0
52	87800.0
53	70738.0
54	70738.0
55	78828.0
56	86918.0
57	73064.0
58	59210.0
59	48833.0
60	38456.0
61	38456.0
62	34560.0
63	30664.0
64	24753.0
65	18842.0
66	16024.0
67	13206.0
68	13206.0
69	11222.0
70	9238.0
71	9292.0
72	9346.0
73	8138.5
74	6931.0
75	6931.0
76	4198.0
77	1465.0
78	1098.5
79	732.0
80	553.5
81	375.0
82	375.0
83	283.5
84	192.0
85	143.0
86	94.0
87	62.5
88	31.0
89	31.0
90	21.0
91	11.0
92	7.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	992553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.15820007318781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.39958634121632	39.825403374310774
2	12.215438087781386	12.254087751771547
3	3.5741095768579574	5.378127097186141
4	1.5346228150674057	3.0789567278011845
5	0.8469114480656901	2.1239776928176046
6	0.5127999949587353	1.543267484680197
7	0.3219529790307337	1.1304007355467665
8	0.2395673542278197	0.9613013827490589
9	0.177174026203643	0.7998057228685089
>10	0.9940572159131038	9.20702316154185
>50	0.09610559813786856	3.3676629087910035
>100	0.07483435204932916	7.70565881688757
>500	0.008627016422992056	3.092004653588142
>1k	0.003410680911415464	3.5545072047790263
>5k	2.00628288906792E-4	0.8786139631087049
>10k+	6.01884866720376E-4	5.099201321571904
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	21946	2.2110658070652147	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	16445	1.6568384761317532	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	12281	1.2373142794389822	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	8731	0.8796507592037907	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	4541	0.45750705503887446	Illumina PCR Primer Index 10 (95% over 22bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3826	0.3854705995548853	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	3755	0.3783173291501814	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2863	0.2884480727981276	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2671	0.26910401761921027	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	2141	0.21570636530240705	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1856	0.1869925333962015	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1819	0.1832647727627643	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1730	0.17429799718503697	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1540	0.15515544258089994	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1489	0.15001717792400002	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1470	0.14810292246358633	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	1302	0.1311768741820336	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1148	0.11566132992394361	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1077	0.10850805951923978	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1052	0.10598930233448492	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	1042	0.10498179946058295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.0150057478038956E-4	0.0
10	0.0	0.0	0.0	3.0225086217058433E-4	0.0
11	0.0	0.0	0.0	5.037514369509739E-4	0.0
12	0.0	0.0	0.0	6.045017243411687E-4	0.0
13	0.0	0.0	0.0	7.052520117313635E-4	0.0
14	0.0	0.0	0.0	7.052520117313635E-4	0.0
15	0.0	0.0	0.0	8.060022991215583E-4	0.0
16	0.0	0.0	0.0	8.060022991215583E-4	0.0
17	0.0	0.0	0.0	8.060022991215583E-4	0.0
18	0.0	0.0	0.0	9.06752586511753E-4	0.0
19	0.0	0.0	0.0	0.0011082531612921426	0.0
20	0.0	0.0	0.0	0.0013097537360725322	0.0
21	0.0	0.0	0.0	0.001813505173023506	0.0
22	0.0	0.0	0.0	0.0021157560351940906	0.0
23	0.0	0.0	0.0	0.0025187571847548693	0.0
24	0.0	0.0	0.0	0.0033247594838764277	0.0
25	0.0	0.0	0.0	0.0034255097712666224	0.0
26	0.0	0.0	0.0	0.003727760633437207	0.0
27	0.0	0.0	0.0	0.004231512070388181	0.0
28	0.0	0.0	0.0	0.010578780175970451	0.0
29	0.0	0.0	0.0	0.020754559202380125	0.0
30	0.0	0.0	0.0	0.035464101161348564	0.0
31	0.0	0.0	0.0	0.08170848307344797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2590	0.0	34.214287	1
TTATACA	2770	0.0	31.723825	2
TATACAC	2945	0.0	30.278439	3
GATTACG	80	1.8189894E-12	30.062502	1
CGTCTTC	625	0.0	28.416	37
ATTACGG	90	5.456968E-12	26.722221	2
GTATCAA	1405	0.0	25.807829	1
CCGTCTT	1855	0.0	25.730457	37
GGTATCA	475	0.0	25.705263	1
GCCGTCT	1885	0.0	25.320955	36
TTACGGG	95	1.0913936E-11	25.31579	3
TGCCGTC	2250	0.0	21.213333	35
ATGCCGT	2355	0.0	20.18896	34
TATGCCG	2570	0.0	18.643969	33
TAGACTG	70	1.2189845E-4	18.5	5
GTCCTAA	120	5.171387E-9	18.5	1
TACACAT	4970	0.0	18.388329	5
ATACACA	4980	0.0	18.23996	4
ACGGTAC	155	2.0008883E-11	17.903227	3
TCGCTGC	240	0.0	16.1875	25
>>END_MODULE