FastQCFastQC Report
Fri 10 Feb 2017
ERR1630974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935243
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT212992.277376040237671No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG161881.7308870528835822No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT148681.5897472635454102TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC85890.9183709474436056No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC59530.6365190650985894RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC41870.44769113481736833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25000.2673102070798712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.23940302146073267No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG20140.21534510282354424No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA16580.1772801293353706No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15070.16113459282774636No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14510.15514684418915728No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC13630.1457375248999458No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC13030.13932207993002887No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT12910.13803899093604552No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12460.13322740720860782No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12400.13258586271161613No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT11900.1272396585700187No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11520.12317654342240467No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10960.11718879478381554No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10940.11697494661815164No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT9780.10457175300964561No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA9490.1014709546075191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23950.034.8371621
TTATACA26250.031.9961912
CGTCTTC8350.030.79640837
TATACAC27900.030.1039433
GATTACG702.0736479E-1029.0714281
GGTATCA4400.026.909091
CCGTCTT25900.025.07142637
GCCGTCT26000.024.97536
TCGCCGA601.3369627E-624.6666661
GTATCAA13700.023.7664221
CGCCGAG652.681958E-622.769232
TGCCGTC30750.021.17723535
CGGCGTA450.003825283120.55555512
ATGCCGT31950.020.20813634
TTACGGG1303.274181E-1119.9230773
TACACAT45600.018.9057035
TATGCCG34150.018.7979533
ATACACA45600.018.6217124
TCGGCGT500.00703392518.511
TCAATAC500.00703392518.53
Produced by FastQC (version 0.11.5)