FastQCFastQC Report
Fri 10 Feb 2017
ERR1630973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174621
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT108220.921318450802429No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG80550.6857531067467719No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA60530.5153151527173445No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT56970.4850075045482755RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44610.3797820743882495No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC43580.3710132885415806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40040.34087590805885476No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC19640.16720286798890877No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC19200.1634569788893609RNA PCR Primer, Index 41 (95% over 23bp)
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA17300.1472815486867679No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14960.12736022938462704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14730.12540215099168156No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC11910.10139440721730669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC250.00549639129.63
GGTATCA7600.028.9671061
GATTACG950.027.2631591
CTTATAC13300.027.2631571
TATACAC14350.027.0731723
TTATACA14200.026.31692
GTATCAA26750.025.5196271
CGTCTTC3250.025.04615437
CCGTCTT10400.024.90384737
GCCGTCT10600.024.43396236
GCTTTAT11550.023.225111
TATACCG400.001931212623.1255
TTAACGG903.8289727E-922.6111135
ATTGAGC11650.020.9613726
TGCCGTC12700.020.39370235
ATGCCGT13000.019.92307734
TTATTGA13200.019.481064
TATTGAG12950.019.2857155
CTTAACG500.00703497118.530
GTCGCAA609.23698E-418.518
Produced by FastQC (version 0.11.5)