##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630973.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174621
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276348711626984	31.0	31.0	34.0	28.0	34.0
2	31.24106499032454	31.0	31.0	34.0	28.0	34.0
3	30.683674989634955	31.0	31.0	34.0	26.0	34.0
4	34.90153334564936	37.0	35.0	37.0	32.0	37.0
5	35.10992907499526	37.0	35.0	37.0	32.0	37.0
6	35.316369279963496	37.0	35.0	37.0	33.0	37.0
7	35.331410727375044	37.0	35.0	37.0	33.0	37.0
8	35.350067809106086	37.0	35.0	37.0	33.0	37.0
9	37.0812968608598	39.0	37.0	39.0	33.0	39.0
10	36.90030656696926	39.0	37.0	39.0	33.0	39.0
11	36.96280076722619	39.0	37.0	39.0	33.0	39.0
12	36.89809734373896	39.0	37.0	39.0	33.0	39.0
13	36.9809896128198	39.0	37.0	39.0	33.0	39.0
14	38.07342623705859	40.0	37.0	41.0	33.0	41.0
15	38.076083264303975	40.0	37.0	41.0	33.0	41.0
16	38.06330382310549	40.0	37.0	41.0	33.0	41.0
17	38.056659126645954	40.0	37.0	41.0	33.0	41.0
18	38.08754823896389	40.0	37.0	41.0	33.0	41.0
19	38.12458316342037	40.0	37.0	41.0	33.0	41.0
20	38.12669533406946	40.0	37.0	41.0	33.0	41.0
21	38.0719985425086	40.0	37.0	41.0	33.0	41.0
22	38.006755370455664	40.0	37.0	41.0	33.0	41.0
23	38.00006640439767	40.0	37.0	41.0	33.0	41.0
24	37.970750565501554	40.0	37.0	41.0	33.0	41.0
25	37.93766755404509	40.0	37.0	41.0	33.0	41.0
26	37.82780573478594	40.0	37.0	41.0	33.0	41.0
27	37.757035673634306	40.0	37.0	41.0	33.0	41.0
28	37.72159445472199	40.0	37.0	41.0	33.0	41.0
29	37.65346609672397	39.0	37.0	41.0	32.0	41.0
30	37.569063553265266	39.0	37.0	41.0	32.0	41.0
31	37.544840420867665	39.0	37.0	41.0	32.0	41.0
32	37.46894104566494	39.0	36.0	41.0	32.0	41.0
33	37.445018435733736	39.0	36.0	41.0	32.0	41.0
34	37.37680409255411	39.0	36.0	41.0	32.0	41.0
35	37.32302419248421	39.0	36.0	40.0	31.0	41.0
36	37.308663815818036	39.0	36.0	40.0	31.0	41.0
37	37.253807823970455	39.0	36.0	40.0	31.0	41.0
38	37.19998535697898	39.0	36.0	40.0	31.0	41.0
39	37.12283621695849	39.0	36.0	40.0	31.0	41.0
40	37.049707948351	39.0	36.0	40.0	31.0	41.0
41	36.99355536807192	39.0	36.0	40.0	31.0	41.0
42	36.93094027775768	39.0	36.0	40.0	31.0	41.0
43	36.28152910598397	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	10.0
11	12.0
12	10.0
13	5.0
14	7.0
15	7.0
16	5.0
17	10.0
18	29.0
19	48.0
20	103.0
21	244.0
22	494.0
23	925.0
24	1648.0
25	2868.0
26	4562.0
27	7130.0
28	10731.0
29	15355.0
30	21274.0
31	28125.0
32	36707.0
33	48119.0
34	63778.0
35	85203.0
36	119348.0
37	176406.0
38	297281.0
39	254168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.457219818137084	21.514173507880415	15.040936608489035	22.987670065493464
2	14.437593061932317	22.74776289543606	39.74413874773225	23.070505294899377
3	15.573704199056545	28.141587797255458	33.689334687529	22.595373316158998
4	11.872765768703267	18.668489666028446	40.31189634784326	29.146848217425024
5	12.649782355329933	38.06802364337092	36.114968147172576	13.167225854126565
6	27.76699888730067	41.27075882348434	17.315116961130443	13.647125328084547
7	25.248229003227422	32.70637933427037	23.64379659481654	18.401595067685662
8	25.359754337782142	34.341204524693495	22.08474052481609	18.21430061270827
9	25.380782397045515	15.097976283413969	20.25487369968696	39.266367619853554
10	14.830996551228012	29.271228762298644	33.753610739123516	22.144163947349828
11	32.43531317761219	24.1150975506142	24.930254098981713	18.519335172791905
12	22.14263153817274	26.433121832488947	29.548424555665186	21.875822073673127
13	28.9159652347438	21.712790763999624	26.359225656616047	23.012018344640527
14	21.707938134938846	21.679333163633206	28.381750368842372	28.230978332585575
15	23.69683497911241	29.37849740469479	23.917161365240364	23.007506250952435
16	23.10115347844113	28.11281255826347	25.0155582098396	23.770475753455795
17	22.231596404287	28.735396353376963	25.847230723782396	23.185776518553645
18	23.577817866358597	26.378210503643302	27.52624037881155	22.517731251186554
19	25.101883926815542	26.281157922427745	26.995686268166498	21.621271882590214
20	24.530380437604983	25.923084978048237	26.539539136453378	23.006995447893406
21	23.89000366926864	26.45372422253646	27.195325130403763	22.460946977791135
22	21.933287417813915	29.38105141998994	27.29714520683693	21.388515955359217
23	23.10489936754068	26.47390094336812	27.38628034063753	23.034919348453673
24	22.908240189814418	27.405946258410157	26.914979384839878	22.770834166935547
25	22.18954028576026	28.772684976686097	26.737134786454526	22.30063995109912
26	23.662270638784765	26.775870685097576	27.00334831405194	22.55851036206572
27	22.251262322059624	28.047089231335043	26.896335073185305	22.80531337342002
28	22.103725371843343	26.73730505414087	28.233872883253408	22.92509669076238
29	22.77134496999458	27.855112414983218	26.619479815191454	22.754062799830756
30	22.802078287379505	27.53500916465822	26.981724317886364	22.681188230075914
31	23.18611705392633	26.42018148832687	27.736350703758916	22.65735075398788
32	21.74675916742507	28.870844297862885	27.54794950882029	21.834447025891755
33	21.344757159969046	27.823698026852917	28.157592959771705	22.673951853406333
34	23.775498650202916	26.676860025489074	27.264538944902228	22.283102379405783
35	21.78549506606812	27.472095254554446	28.521880674702732	22.2205290046747
36	22.615464903147483	26.646807778849517	27.0393599297135	23.698367388289498
37	22.629256585741274	26.7315159528052	28.228679718819944	22.410547742633582
38	22.781305629645647	26.012390379535187	28.21029080869489	22.99601318212428
39	23.545892675169267	26.177635169131147	27.389260025148538	22.887212130551045
40	21.985133928305387	26.00498373517926	29.7075397085528	22.302342627962553
41	22.23772604099535	26.01698760706645	27.26079305580268	24.484493296135522
42	22.35137972162936	26.02422398373603	28.41665524454271	23.2077410500919
43	22.86712054356256	26.033844108014414	28.602502424186184	22.49653292423684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	37.0
2	44.0
3	162.5
4	281.0
5	281.0
6	367.0
7	453.0
8	455.0
9	457.0
10	661.0
11	865.0
12	865.0
13	1375.0
14	1885.0
15	4319.0
16	6753.0
17	7818.5
18	8884.0
19	8884.0
20	9077.5
21	9271.0
22	11652.0
23	14033.0
24	18321.0
25	22609.0
26	22609.0
27	27718.5
28	32828.0
29	38413.5
30	43999.0
31	49224.0
32	54449.0
33	54449.0
34	59252.5
35	64056.0
36	68134.5
37	72213.0
38	75056.0
39	77899.0
40	77899.0
41	78564.5
42	79230.0
43	79487.0
44	79744.0
45	80905.0
46	82066.0
47	82066.0
48	82927.0
49	83788.0
50	89279.5
51	94771.0
52	83115.5
53	71460.0
54	71460.0
55	74599.0
56	77738.0
57	67171.5
58	56605.0
59	49180.5
60	41756.0
61	41756.0
62	37868.0
63	33980.0
64	29545.5
65	25111.0
66	20488.0
67	15865.0
68	15865.0
69	13053.0
70	10241.0
71	8748.5
72	7256.0
73	4748.0
74	2240.0
75	2240.0
76	1568.5
77	897.0
78	670.0
79	443.0
80	329.0
81	215.0
82	215.0
83	155.5
84	96.0
85	78.5
86	61.0
87	50.5
88	40.0
89	40.0
90	24.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1174621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.68967798507633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.85732079375843	41.27799196580314
2	12.39733786870214	12.816288046108124
3	3.3690896596016615	5.22441478822981
4	1.304791590357573	2.697770285728744
5	0.6742040540566435	1.7424695225210447
6	0.4159139647959018	1.2899075345877924
7	0.2863880418119823	1.036231396202656
8	0.19220796718380034	0.7948134343917408
9	0.15196530195443123	0.7069533770636498
>10	1.100957740724359	11.430526700614417
>50	0.14752789918052567	5.333044550920291
>100	0.09434986714552503	8.971981554647137
>500	0.004965781704457153	1.661572981249294
>1k	0.002317364795413338	2.395591241091606
>5k	4.965781704457154E-4	1.694513537262733
>10k+	1.6552605681523843E-4	0.9259290835777476
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	10822	0.921318450802429	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	8055	0.6857531067467719	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	6053	0.5153151527173445	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	5697	0.4850075045482755	RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4461	0.3797820743882495	No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	4358	0.3710132885415806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4004	0.34087590805885476	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	1964	0.16720286798890877	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	1920	0.1634569788893609	RNA PCR Primer, Index 41 (95% over 23bp)
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1730	0.1472815486867679	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1496	0.12736022938462704	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1473	0.12540215099168156	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1191	0.10139440721730669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.7026768634308427E-4	0.0
12	0.0	0.0	0.0	1.7026768634308427E-4	0.0
13	0.0	0.0	0.0	1.7026768634308427E-4	0.0
14	0.0	0.0	0.0	2.554015295146264E-4	0.0
15	0.0	0.0	0.0	2.554015295146264E-4	0.0
16	0.0	0.0	0.0	2.554015295146264E-4	0.0
17	0.0	0.0	0.0	2.554015295146264E-4	0.0
18	1.7026768634308427E-4	0.0	0.0	3.4053537268616854E-4	0.0
19	1.7026768634308427E-4	0.0	0.0	5.95936902200795E-4	0.0
20	1.7026768634308427E-4	0.0	0.0	6.810707453723371E-4	0.0
21	1.7026768634308427E-4	0.0	0.0	0.0011067399612300478	0.0
22	1.7026768634308427E-4	0.0	0.0	0.0013621414907446742	0.0
23	1.7026768634308427E-4	0.0	0.0	0.001872944549773927	0.0
24	1.7026768634308427E-4	0.0	0.0	0.0022986137656316376	0.0
25	1.7026768634308427E-4	0.0	0.0	0.002468881451974722	0.0
26	1.7026768634308427E-4	0.0	0.0	0.0028094168246608907	0.0
27	1.7026768634308427E-4	0.0	0.0	0.0038310229427193964	0.0
28	1.7026768634308427E-4	0.0	0.0	0.01038632886692814	0.0
29	1.7026768634308427E-4	0.0	0.0	0.026050956010491896	0.0
30	1.7026768634308427E-4	0.0	0.0	0.04903709366680827	0.0
31	1.7026768634308427E-4	0.0	0.0	0.10846051620054468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGC	25	0.005496391	29.6	3
GGTATCA	760	0.0	28.967106	1
GATTACG	95	0.0	27.263159	1
CTTATAC	1330	0.0	27.263157	1
TATACAC	1435	0.0	27.073172	3
TTATACA	1420	0.0	26.3169	2
GTATCAA	2675	0.0	25.519627	1
CGTCTTC	325	0.0	25.046154	37
CCGTCTT	1040	0.0	24.903847	37
GCCGTCT	1060	0.0	24.433962	36
GCTTTAT	1155	0.0	23.22511	1
TATACCG	40	0.0019312126	23.125	5
TTAACGG	90	3.8289727E-9	22.61111	35
ATTGAGC	1165	0.0	20.961372	6
TGCCGTC	1270	0.0	20.393702	35
ATGCCGT	1300	0.0	19.923077	34
TTATTGA	1320	0.0	19.48106	4
TATTGAG	1295	0.0	19.285715	5
CTTAACG	50	0.007034971	18.5	30
GTCGCAA	60	9.23698E-4	18.5	18
>>END_MODULE