FastQCFastQC Report
Fri 10 Feb 2017
ERR1630972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880065
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT165191.8770204473533205No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG124121.410350371847534No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT100591.1429837568815941RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC65010.7386954372688381No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC37110.42167339912392837RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC30920.3513376852846097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23450.26645759120064993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.24895888371881622No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16850.19146313056421968No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG15380.17475981887701478No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA12770.14510291853442644No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT9620.10931010777613018No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9240.10499224489100237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22100.032.3122181
TTATACA22600.031.8429182
CGTCTTC4450.031.17977337
TATACAC24150.030.3354033
CCGTCTT15450.026.46278237
CGCACTC358.8677363E-426.42857234
TTACGGG509.087931E-625.8999983
GCCGTCT15850.025.79495236
TTAACGG1051.8189894E-1224.66666835
GGTATCA5050.023.8118821
GATTACG551.9020017E-523.5454541
AACGGCC1200.023.12499837
TAACGGC1201.0913936E-1121.58333236
TGCCGTC19000.021.51842135
GTATCAA12700.021.4133851
TAAACCG450.003825106720.5555555
ATGCCGT20050.020.39152134
GTCGCCC656.899779E-519.92307737
ATACACA37950.019.6455864
TACACAT37550.019.608525
Produced by FastQC (version 0.11.5)