FastQCFastQC Report
Fri 10 Feb 2017
ERR1630971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949812
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG113031.1900249733631498No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT111821.177285610204967No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT80230.8446934761826551TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC65740.692136970263589No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC28690.30205977603989No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC27660.2912155247564781Illumina Paired End PCR Primer 2 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23460.24699624767848793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.22846626490294922No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG16520.17392915650676133No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11870.12497209974184365No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA9540.10044092936286339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15550.030.4565931
TATACAC17900.026.9748613
TTATACA18200.026.1236252
GGTATCA5450.025.7981641
CGTCTTC4900.025.2959237
CCGTCTT13500.023.70740937
GTATCAA13850.022.9747281
GCCGTCT13950.022.94265236
TGCCGTC16700.019.1646735
TTAACGG500.00703400618.49999835
AATACGC500.00703400618.4999985
ATGCCGT17150.018.44606434
TATGCCG18600.017.00806433
GTATGTA1352.2177119E-816.4444451
TACACAT30950.016.0193845
ATACACA32150.015.6516334
TATACTG2250.015.6222215
GTATAAT2650.015.3584911
ATGGGCG750.00410370714.811
TAGCACG750.00410370714.85
Produced by FastQC (version 0.11.5)