##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630971.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	949812
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.050894282236907	31.0	31.0	34.0	28.0	34.0
2	31.029611123043296	31.0	31.0	34.0	28.0	34.0
3	30.456440853558387	31.0	30.0	34.0	26.0	34.0
4	34.698937263374226	35.0	35.0	37.0	32.0	37.0
5	34.83278164520979	35.0	35.0	37.0	32.0	37.0
6	35.04463620169044	36.0	35.0	37.0	32.0	37.0
7	35.06082045710098	36.0	35.0	37.0	32.0	37.0
8	35.09350166138141	36.0	35.0	37.0	32.0	37.0
9	36.80912327913313	39.0	37.0	39.0	33.0	39.0
10	36.57799859340585	38.0	35.0	39.0	32.0	39.0
11	36.664717859955445	38.0	35.0	39.0	32.0	39.0
12	36.58085284245725	38.0	35.0	39.0	32.0	39.0
13	36.676834994714746	38.0	35.0	39.0	32.0	39.0
14	37.714930954757364	39.0	37.0	40.0	33.0	41.0
15	37.70633241104555	39.0	37.0	40.0	33.0	41.0
16	37.70691673720694	39.0	37.0	40.0	33.0	41.0
17	37.66447886529124	39.0	37.0	40.0	32.0	41.0
18	37.70701149280068	39.0	37.0	40.0	33.0	41.0
19	37.725963664388324	39.0	37.0	40.0	32.0	41.0
20	37.7403117669602	39.0	37.0	40.0	32.0	41.0
21	37.69084197714916	39.0	37.0	40.0	32.0	41.0
22	37.599162781687326	39.0	37.0	40.0	32.0	41.0
23	37.59852897204921	39.0	37.0	40.0	32.0	41.0
24	37.5410986595242	39.0	37.0	40.0	32.0	41.0
25	37.49521589535613	39.0	36.0	40.0	32.0	41.0
26	37.367669601984396	39.0	36.0	40.0	32.0	41.0
27	37.27974693939432	39.0	36.0	40.0	32.0	41.0
28	37.264245977098625	39.0	36.0	40.0	32.0	41.0
29	37.1646231043617	39.0	36.0	40.0	31.0	41.0
30	37.07651830046367	39.0	36.0	40.0	31.0	41.0
31	37.08652764968225	39.0	36.0	40.0	31.0	41.0
32	36.95895187679246	39.0	36.0	40.0	31.0	41.0
33	36.95150303428468	39.0	36.0	40.0	31.0	41.0
34	36.84991345655772	39.0	36.0	40.0	30.0	41.0
35	36.80925593696437	39.0	36.0	40.0	30.0	41.0
36	36.81403477740858	39.0	36.0	40.0	30.0	41.0
37	36.71868854046906	39.0	35.0	40.0	30.0	41.0
38	36.680802095572595	39.0	35.0	40.0	30.0	41.0
39	36.622005196817895	39.0	35.0	40.0	30.0	41.0
40	36.54354861804231	38.0	35.0	40.0	30.0	41.0
41	36.493541879866754	38.0	35.0	40.0	30.0	41.0
42	36.44458903446156	38.0	35.0	40.0	30.0	41.0
43	35.6965557394516	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	10.0
11	14.0
12	10.0
13	8.0
14	9.0
15	14.0
16	12.0
17	18.0
18	27.0
19	51.0
20	146.0
21	312.0
22	490.0
23	1022.0
24	1654.0
25	2973.0
26	4793.0
27	7252.0
28	10612.0
29	14893.0
30	19984.0
31	26734.0
32	34878.0
33	44849.0
34	59207.0
35	79410.0
36	109624.0
37	159368.0
38	237969.0
39	133461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.304001212871604	22.39506344413421	15.251018096212723	24.04991724678147
2	14.587413088063744	22.560885733176672	39.61426050628967	23.23744067246992
3	15.846609644856036	28.124302493546093	33.26489873785549	22.76418912374238
4	12.4724682358193	19.332352086518174	37.67440293447545	30.52077674318707
5	12.95435307197635	38.04152821821582	35.437539218287405	13.566579491520429
6	28.21653127145161	39.83325121181876	17.814367474826597	14.135850041903028
7	24.840810602519237	33.26574100979983	22.739657953363402	19.15379043431753
8	24.862077969113887	35.26066210997545	22.049100242995458	17.82815967791521
9	24.416094974584443	15.154367390599402	19.81044669892568	40.619090935890476
10	14.5226634323424	28.63808837959512	33.70403827283715	23.13520991522533
11	32.335346363280316	23.040349037493733	24.978311497433175	19.645993101792776
12	21.080171655022255	26.028519328035443	29.83379868858258	23.05751032835972
13	28.9762605652487	21.216935561984897	26.43154645340341	23.375257419362992
14	21.624279331067623	21.961082824811648	26.998079619966898	29.41655822415383
15	23.66352499231427	28.76211292339958	23.135525767204456	24.438836317081698
16	24.00833006952955	27.66452729592804	24.027070620291173	24.300072014251242
17	21.366859968077893	29.74967677814136	25.519997641638554	23.363465612142193
18	23.671105439813353	26.48839980964654	26.13327690111306	23.707217849427046
19	23.839770396667973	28.27138423182693	25.729512787793794	22.159332583711304
20	22.864945905084376	26.383431668582837	26.08368814038989	24.667934285942902
21	24.41956934635486	26.31036457741111	26.718129482466	22.55193659376803
22	22.678066817433347	29.33296273367782	25.998829241997363	21.99014120689147
23	23.365887143982178	26.72097215027816	26.409015678892246	23.50412502684742
24	24.16214998336513	27.808239946431506	25.757413045950145	22.27219702425322
25	22.44159896905914	27.324459998399682	26.100217727297615	24.133723305243564
26	24.572231136266968	27.58377447326418	26.374166677195067	21.469827713273784
27	22.73660471756516	28.244010393635794	25.90544234016837	23.113942548630675
28	22.1086909830577	26.643167279419504	28.04439194282658	23.20374979469621
29	24.17152025874594	27.289084576737295	25.88564894947632	22.65374621504045
30	22.161964683537374	26.981234181080044	27.19769807077611	23.65910306460647
31	24.855234509566102	25.469040189005828	27.067672339368208	22.608052962059862
32	23.688582582658462	26.414279878544384	26.81846512783582	23.078672410961325
33	23.068880999608343	27.152531237760734	27.71074696887384	22.067840793757078
34	23.39189229026376	27.455117433765842	26.333000635915315	22.819989640055084
35	22.0352027559138	26.024834388278943	29.07807018652112	22.86189266928613
36	24.433361549443468	26.057367142129177	25.7705735450805	23.738697763346853
37	22.733340913780832	26.63148075619175	27.604410135900576	23.03076819412684
38	22.449284700551267	25.579798949686882	27.83582435260873	24.135091997153122
39	23.952003133251633	26.013463717030316	26.594210222654592	23.440322927063463
40	22.03920354764943	25.616964199231006	29.94192534943757	22.401906903681994
41	22.93201180865266	26.075370704939505	26.490821341486527	24.50179614492131
42	22.820937195992467	25.575166453993003	28.619031976854366	22.984864373160164
43	23.230176076949967	25.159084113487722	28.442154868542406	23.16858494101991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	14.5
2	20.0
3	63.0
4	106.0
5	106.0
6	148.0
7	190.0
8	203.5
9	217.0
10	349.0
11	481.0
12	481.0
13	749.5
14	1018.0
15	2441.5
16	3865.0
17	4491.0
18	5117.0
19	5117.0
20	5433.0
21	5749.0
22	7253.0
23	8757.0
24	11608.5
25	14460.0
26	14460.0
27	18331.5
28	22203.0
29	26293.0
30	30383.0
31	35158.5
32	39934.0
33	39934.0
34	44954.0
35	49974.0
36	53920.5
37	57867.0
38	61014.5
39	64162.0
40	64162.0
41	65792.0
42	67422.0
43	67925.5
44	68429.0
45	69201.5
46	69974.0
47	69974.0
48	69424.0
49	68874.0
50	67654.5
51	66435.0
52	74884.0
53	83333.0
54	83333.0
55	68384.0
56	53435.0
57	55707.0
58	57979.0
59	48919.5
60	39860.0
61	39860.0
62	32321.0
63	24782.0
64	20819.0
65	16856.0
66	14007.0
67	11158.0
68	11158.0
69	9107.0
70	7056.0
71	5680.5
72	4305.0
73	3405.5
74	2506.0
75	2506.0
76	1930.5
77	1355.0
78	1047.0
79	739.0
80	597.5
81	456.0
82	456.0
83	317.0
84	178.0
85	135.0
86	92.0
87	63.0
88	34.0
89	34.0
90	31.0
91	28.0
92	19.0
93	10.0
94	6.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	949812.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.94335275321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76580947328092	48.611958643754996
2	12.305885938139593	14.999238953376153
3	3.652909823505826	6.67861715848745
4	1.5036306087335198	3.6654516239428316
5	0.7645946347484482	2.3298480269343216
6	0.4619719377471596	1.6892471258525468
7	0.32038519863165704	1.366774372398143
8	0.21794365763143264	1.0625773765885782
9	0.14686017728469863	0.8055136430694313
>10	0.785743010259861	8.571586312303907
>50	0.04957156430556364	2.0162462439908233
>100	0.021412958177923	2.250033663042983
>500	0.0015541662095001534	0.6833240381451862
>1k	0.0010361108063334356	1.367069036732784
>5k	3.453702687778119E-4	1.5361898944717822
>10k+	3.453702687778119E-4	2.3663238869081336
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	11303	1.1900249733631498	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	11182	1.177285610204967	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	8023	0.8446934761826551	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	6574	0.692136970263589	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	2869	0.30205977603989	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	2766	0.2912155247564781	Illumina Paired End PCR Primer 2 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2346	0.24699624767848793	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2170	0.22846626490294922	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1652	0.17392915650676133	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1187	0.12497209974184365	No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA	954	0.10044092936286339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.0528399304283374E-4	0.0
13	0.0	0.0	0.0	1.0528399304283374E-4	0.0
14	0.0	0.0	0.0	1.0528399304283374E-4	0.0
15	0.0	0.0	0.0	1.0528399304283374E-4	0.0
16	0.0	0.0	0.0	1.0528399304283374E-4	0.0
17	0.0	0.0	0.0	1.0528399304283374E-4	0.0
18	0.0	0.0	0.0	1.0528399304283374E-4	0.0
19	0.0	0.0	0.0	1.0528399304283374E-4	0.0
20	0.0	0.0	0.0	2.1056798608566748E-4	0.0
21	0.0	0.0	0.0	2.1056798608566748E-4	0.0
22	0.0	0.0	0.0	3.1585197912850123E-4	0.0
23	0.0	0.0	0.0	9.475559373855036E-4	0.0
24	0.0	0.0	0.0	0.0011581239234711711	0.0
25	0.0	0.0	0.0	0.001263407916514005	0.0
26	0.0	0.0	0.0	0.0014739759025996723	0.0
27	0.0	0.0	0.0	0.0026320998260708434	0.0
28	0.0	0.0	0.0	0.007791015485169697	0.0
29	0.0	0.0	0.0	0.01800356281032457	0.0
30	0.0	0.0	0.0	0.03200633388502146	0.0
31	0.0	0.0	0.0	0.07496220304649762	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1555	0.0	30.456593	1
TATACAC	1790	0.0	26.974861	3
TTATACA	1820	0.0	26.123625	2
GGTATCA	545	0.0	25.798164	1
CGTCTTC	490	0.0	25.29592	37
CCGTCTT	1350	0.0	23.707409	37
GTATCAA	1385	0.0	22.974728	1
GCCGTCT	1395	0.0	22.942652	36
TGCCGTC	1670	0.0	19.16467	35
TTAACGG	50	0.007034006	18.499998	35
AATACGC	50	0.007034006	18.499998	5
ATGCCGT	1715	0.0	18.446064	34
TATGCCG	1860	0.0	17.008064	33
GTATGTA	135	2.2177119E-8	16.444445	1
TACACAT	3095	0.0	16.019384	5
ATACACA	3215	0.0	15.651633	4
TATACTG	225	0.0	15.622221	5
GTATAAT	265	0.0	15.358491	1
ATGGGCG	75	0.004103707	14.8	11
TAGCACG	75	0.004103707	14.8	5
>>END_MODULE