FastQCFastQC Report
Fri 10 Feb 2017
ERR1630970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472923
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG130892.767680996695022No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT107432.271617155435451TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT86541.8298961987469418No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC70531.4913632874696303No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC42310.8946488117515959RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC30870.6527489675909186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.3975277159283858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17890.3782856828701501No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG17450.3689818427101241No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA17250.3647528244555667No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA11060.23386470947701846No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT9980.21102801090240905No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8910.18840276324052752No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7020.14843854073496107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6820.14420952248040378No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5100.10783996549121105No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA4820.10191933993483084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10700.033.5420571
CGTCTTC5600.031.0535737
TCGCCGA303.5963042E-430.8333321
TTATACA11600.030.4612062
AGCAACG250.00549311429.5999982
GCAACGC358.861646E-426.428573
TATACAC13950.026.1254483
GGTATCA3200.025.4375021
CCGTCTT17100.024.88304137
GCCGTCT17500.024.4236
GCTTTAT2850.024.0175441
GTATCAA8050.023.9006211
CCAAACG551.8995903E-523.5454549
CTCCGCT400.001929515623.12500227
AACGGCC400.001929515623.12500237
TTAACGG400.001929515623.12500235
CGCCGAG400.001929515623.1250022
TATTGAG3250.022.1999995
ACGCCCC603.72032E-521.58333213
CGCCCCT603.72032E-521.58333214
Produced by FastQC (version 0.11.5)