FastQCFastQC Report
Fri 10 Feb 2017
ERR1630969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155709
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT150661.303615356460839No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG112440.9729092704132268No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT69340.5999780221491742TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC62300.5390630340336539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49770.430644738424638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46810.4050327547851579No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC28520.24677492344526175No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA26810.23197881127515665No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC20840.18032220913742125RNA PCR Primer, Index 7 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16780.14519225860489102No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG15730.13610692657061596No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14360.12425273144018088No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14100.12200303017455086No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12080.10452458188004074No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11700.10123655695335071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20700.030.207731
GGTATCA8250.029.1515161
TATACAC22350.028.8053683
CGTCTTC3400.028.29411937
GATTACG1050.028.1904771
TTATACA22650.027.7704222
TTACGGG1300.025.6153853
GTATCAA26000.024.051
CCGTCTT10300.023.70873637
GCCGTCT10850.022.6774236
GGACGTA603.7263104E-521.58333227
CATGCGG450.003825819820.55555710
TAGCGAG555.143118E-420.18181828
GACGTAT656.902172E-519.92307728
ACGTATA759.265841E-619.73333429
TTTACCG851.2453202E-619.58823630
AGGACCG1002.874949E-718.55
TGCCGTC13850.018.03249235
TACACAT36250.017.8620685
ATACACA36600.017.640714
Produced by FastQC (version 0.11.5)