##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630969.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155709
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375984785097287	31.0	31.0	34.0	28.0	34.0
2	31.343105401100104	31.0	31.0	34.0	28.0	34.0
3	30.80117832430136	31.0	31.0	34.0	26.0	34.0
4	34.9918154137417	37.0	35.0	37.0	32.0	37.0
5	35.19765961846797	37.0	35.0	37.0	32.0	37.0
6	35.39325037704128	37.0	35.0	37.0	33.0	37.0
7	35.41078333732799	37.0	35.0	37.0	33.0	37.0
8	35.43116995714319	37.0	35.0	37.0	33.0	37.0
9	37.19009543059715	39.0	37.0	39.0	34.0	39.0
10	37.00677160080954	39.0	37.0	39.0	33.0	39.0
11	37.08043633821317	39.0	37.0	39.0	33.0	39.0
12	37.00836369708984	39.0	37.0	39.0	33.0	39.0
13	37.076750289216406	39.0	37.0	39.0	33.0	39.0
14	38.18159242508278	40.0	38.0	41.0	33.0	41.0
15	38.18486920150315	40.0	37.0	41.0	33.0	41.0
16	38.188827810460936	40.0	38.0	41.0	33.0	41.0
17	38.167957504873634	40.0	38.0	41.0	33.0	41.0
18	38.212863272675044	40.0	38.0	41.0	33.0	41.0
19	38.241553885969566	40.0	38.0	41.0	34.0	41.0
20	38.2341895754035	40.0	38.0	41.0	34.0	41.0
21	38.19305205722202	40.0	38.0	41.0	34.0	41.0
22	38.11970054745615	40.0	37.0	41.0	33.0	41.0
23	38.116980139464175	40.0	37.0	41.0	33.0	41.0
24	38.07831902321432	40.0	37.0	41.0	33.0	41.0
25	38.052254503512565	40.0	37.0	41.0	33.0	41.0
26	37.943369827525785	40.0	37.0	41.0	33.0	41.0
27	37.85050215928058	40.0	37.0	41.0	33.0	41.0
28	37.83876477556201	40.0	37.0	41.0	33.0	41.0
29	37.77674137693831	40.0	37.0	41.0	33.0	41.0
30	37.66159733981478	39.0	37.0	41.0	32.0	41.0
31	37.64377624471212	39.0	37.0	41.0	32.0	41.0
32	37.55790255159387	39.0	37.0	41.0	32.0	41.0
33	37.531035061594224	39.0	37.0	41.0	32.0	41.0
34	37.45974116321669	39.0	36.0	41.0	32.0	41.0
35	37.419488815956264	39.0	36.0	41.0	32.0	41.0
36	37.37937404658093	39.0	36.0	41.0	32.0	41.0
37	37.30512784792712	39.0	36.0	41.0	31.0	41.0
38	37.26012863099621	39.0	36.0	41.0	31.0	41.0
39	37.179688831704176	39.0	36.0	41.0	31.0	41.0
40	37.10055818549479	39.0	36.0	41.0	31.0	41.0
41	37.025052154132226	39.0	36.0	40.0	31.0	41.0
42	36.940426179946684	39.0	36.0	40.0	31.0	41.0
43	36.26502173124896	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	10.0
11	6.0
12	7.0
13	9.0
14	7.0
15	16.0
16	13.0
17	22.0
18	29.0
19	52.0
20	112.0
21	273.0
22	471.0
23	916.0
24	1599.0
25	2717.0
26	4298.0
27	6827.0
28	10169.0
29	14630.0
30	19925.0
31	26769.0
32	35133.0
33	45292.0
34	60167.0
35	81371.0
36	112635.0
37	166914.0
38	285325.0
39	279982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.25711403130027	21.740420815274433	15.05543350445484	23.94703164897046
2	14.214910500826766	22.684862711980262	40.570593462541176	22.529633324651794
3	15.113320048558936	28.536768338742714	33.609931219710155	22.7399803929882
4	11.888632865193575	19.20249820672851	38.65852044069917	30.25034848737874
5	12.743778927048243	38.209099349403694	35.441361103876496	13.605760619671562
6	27.740374090709686	40.50396769428983	17.551823166558364	14.203835048442127
7	25.005169986562358	32.726058203232824	22.93881937408119	19.329952436123627
8	25.111684688792767	34.96061724880571	21.871768758398524	18.055929304002998
9	24.94044781168962	14.832453498242204	19.969040649506063	40.25805804056211
10	14.802169058127953	28.991121467428222	33.42606140473078	22.78064806971305
11	32.19980116101891	23.475113545018687	25.124923315471282	19.200161978491124
12	21.539072552000547	26.266819761722026	29.758356125979812	22.435751560297618
13	29.18822990908611	21.441470127860907	26.670121977072082	22.7001779859809
14	21.743362732314104	21.902572360343303	27.66172107338439	28.6923438339582
15	23.707438464180864	28.651935738148616	23.713322298260202	23.927303499410318
16	23.5419988941853	27.504588092677306	24.28258324543635	24.67082976770104
17	21.594449813923745	29.1828652368373	25.678522880759775	23.544162068479178
18	24.092483488490615	26.002047228151724	26.720480674633496	23.18498860872417
19	24.407874300537593	26.81782351785787	27.262658679650325	21.51164350195421
20	23.70908247664421	26.40136920280105	26.66501688573854	23.224531434816203
21	24.72464954413265	25.84612562504921	26.67271778622473	22.756507044593405
22	21.985811307171616	29.071937659047393	26.85857772155447	22.08367331222652
23	23.67767318589714	25.734678885428767	27.165229309454197	23.42241861921989
24	22.837323236212576	26.85788550578043	27.396775485870577	22.908015772136412
25	22.401140771595617	27.952365171509435	27.28351167984328	22.36298237705166
26	23.94729122988572	26.329119181385625	27.220952679264414	22.502636909464233
27	22.057715220700018	28.86998370697122	26.825524418344067	22.246776653984696
28	23.078127798606744	26.10622570214474	27.454488976031165	23.36115752321735
29	22.13195536246581	26.174149374972423	27.96421936663987	23.729675895921897
30	22.799770530470905	27.431559328516087	27.882624432274906	21.8860457087381
31	24.277478154102806	25.51230456801842	26.328946127442116	23.88127115043666
32	21.86285648030776	26.299267376130146	28.378164399515793	23.459711744046295
33	23.034345150898712	25.91240528541354	28.204850875090532	22.848398688597214
34	21.730124105635586	27.00402956107463	27.16055685297943	24.105289480310354
35	21.592892328432157	26.376709015850874	30.45974375902584	21.57065489669112
36	22.87876965568322	26.276337728615072	26.39098596619045	24.453906649511254
37	22.808077119759385	26.989925664678566	27.803884887977855	22.398112327584194
38	22.39084406195677	25.779240275882596	28.040709209671295	23.789206452489335
39	23.293233850389676	26.063221797182507	27.40127488840184	23.242269464025977
40	21.864846600658126	25.882207372271047	29.9641172648132	22.288828762257626
41	22.814653169612768	25.68570461941544	27.110284682389775	24.389357528582025
42	23.001378374660057	25.603071361389418	28.566706670969943	22.828843592980586
43	23.111613736675928	25.17908919978991	28.767795353328562	22.941501710205596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	22.5
2	25.0
3	147.5
4	270.0
5	270.0
6	314.5
7	359.0
8	404.5
9	450.0
10	669.0
11	888.0
12	888.0
13	1407.5
14	1927.0
15	4782.0
16	7637.0
17	8508.0
18	9379.0
19	9379.0
20	9251.5
21	9124.0
22	10618.5
23	12113.0
24	15839.0
25	19565.0
26	19565.0
27	24261.5
28	28958.0
29	34038.0
30	39118.0
31	44190.5
32	49263.0
33	49263.0
34	54109.0
35	58955.0
36	62367.5
37	65780.0
38	69218.0
39	72656.0
40	72656.0
41	74765.5
42	76875.0
43	78251.5
44	79628.0
45	81404.5
46	83181.0
47	83181.0
48	84568.0
49	85955.0
50	84239.0
51	82523.0
52	94315.0
53	106107.0
54	106107.0
55	87990.5
56	69874.0
57	69633.0
58	69392.0
59	57345.0
60	45298.0
61	45298.0
62	37832.0
63	30366.0
64	25077.5
65	19789.0
66	16369.0
67	12949.0
68	12949.0
69	10707.0
70	8465.0
71	6486.5
72	4508.0
73	3376.5
74	2245.0
75	2245.0
76	1648.5
77	1052.0
78	783.5
79	515.0
80	389.0
81	263.0
82	263.0
83	188.0
84	113.0
85	91.0
86	69.0
87	53.0
88	37.0
89	37.0
90	25.5
91	14.0
92	8.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1155709.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.12846840094984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22446978823308	39.48368863347781
2	13.522690873131745	13.827889460054587
3	4.061351199804675	6.229519994531193
4	1.6525671157056017	3.379729022232107
5	0.8531365736841783	2.180978317465307
6	0.4859782399946256	1.490839385226866
7	0.338823598617887	1.2126472158801527
8	0.2437202809792801	0.9968835747775793
9	0.17840344877467362	0.8209345583966737
>10	1.2310933151279446	12.380011339869368
>50	0.13031686401786235	4.56783443639492
>100	0.07099464472802533	6.283977486320038
>500	0.0032270286002214115	1.1008508626155769
>1k	0.0025476541580695357	2.616355260688232
>5k	3.396872210759381E-4	1.1431411813103614
>10k+	3.396872210759381E-4	2.2847192707593145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	15066	1.303615356460839	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	11244	0.9729092704132268	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	6934	0.5999780221491742	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	6230	0.5390630340336539	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4977	0.430644738424638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4681	0.4050327547851579	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	2852	0.24677492344526175	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2681	0.23197881127515665	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2084	0.18032220913742125	RNA PCR Primer, Index 7 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1678	0.14519225860489102	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1573	0.13610692657061596	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1436	0.12425273144018088	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1410	0.12200303017455086	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1208	0.10452458188004074	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1170	0.10123655695335071	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	8.652697175500061E-5	0.0
17	0.0	0.0	0.0	1.7305394351000122E-4	0.0
18	0.0	0.0	0.0	3.4610788702000244E-4	0.0
19	0.0	0.0	0.0	3.4610788702000244E-4	0.0
20	0.0	0.0	0.0	6.056888022850042E-4	0.0
21	0.0	0.0	0.0	9.517966893050067E-4	0.0
22	0.0	0.0	0.0	0.0016440124633450117	0.0
23	0.0	0.0	0.0	0.0019035933786100134	0.0
24	0.0	0.0	0.0	0.002422755209140017	0.0
25	0.0	0.0	0.0	0.002682336124405019	0.0
26	0.0	0.0	0.0	0.003114970983180022	0.0
27	0.0	0.0	0.0	0.00423982161599503	0.0
28	0.0	0.0	0.0	0.01142156027166008	0.0
29	0.0	0.0	0.0	0.027255996102825194	0.0
30	0.0	0.0	0.0	0.04793594235227034	0.0
31	0.0	0.0	0.0	0.09595841167629568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2070	0.0	30.20773	1
GGTATCA	825	0.0	29.151516	1
TATACAC	2235	0.0	28.805368	3
CGTCTTC	340	0.0	28.294119	37
GATTACG	105	0.0	28.190477	1
TTATACA	2265	0.0	27.770422	2
TTACGGG	130	0.0	25.615385	3
GTATCAA	2600	0.0	24.05	1
CCGTCTT	1030	0.0	23.708736	37
GCCGTCT	1085	0.0	22.67742	36
GGACGTA	60	3.7263104E-5	21.583332	27
CATGCGG	45	0.0038258198	20.555557	10
TAGCGAG	55	5.143118E-4	20.181818	28
GACGTAT	65	6.902172E-5	19.923077	28
ACGTATA	75	9.265841E-6	19.733334	29
TTTACCG	85	1.2453202E-6	19.588236	30
AGGACCG	100	2.874949E-7	18.5	5
TGCCGTC	1385	0.0	18.032492	35
TACACAT	3625	0.0	17.862068	5
ATACACA	3660	0.0	17.64071	4
>>END_MODULE