FastQCFastQC Report
Fri 10 Feb 2017
ERR1630968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541151
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT213033.936609190410809No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG193233.570722404652306No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT187933.472783012504828RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC100181.8512393028932774No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC78911.458188195161794RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC54151.0006449216577258RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG22640.4183675166450769No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA20150.37235448146635597No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT15730.2906767242414779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13960.25796866309033895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.25408804566562754No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12400.22914121936391135No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT7960.14709387952715602Illumina PCR Primer Index 11 (95% over 24bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT6160.11383144445820112Illumina PCR Primer Index 11 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC24250.035.3216481
CGTCTTC11350.033.08810837
TTATACA26450.032.243862
TTACTCG352.3859906E-531.71428519
TATACAC28650.030.1553233
GATACAC405.9344093E-527.7500023
ATTCCGG405.9344093E-527.75000237
GCTCTAG905.456968E-1226.7222211
CGTTACT358.863308E-426.4285717
ACCGTTA358.863308E-426.428578
CGCCGAG509.079487E-625.8999982
GTATCAA5750.023.8086971
CCGTCTT31500.023.66825337
GGTATCA2050.023.4634131
GCCGTCT32150.023.18973436
TCGGCGT400.001929873623.12500211
CGCACCA502.699543E-422.19999918
CCGCCTA502.699543E-422.19999915
GCCGAGC603.7215977E-521.5833323
CGGCGTA450.003823231420.55555512
Produced by FastQC (version 0.11.5)