##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630968.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541151
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198314333707227	31.0	31.0	34.0	28.0	34.0
2	31.245443508373818	31.0	31.0	34.0	28.0	34.0
3	30.709454477585737	31.0	31.0	34.0	26.0	34.0
4	34.87237388455348	37.0	35.0	37.0	32.0	37.0
5	35.03815386093715	35.0	35.0	37.0	32.0	37.0
6	35.233962424535854	37.0	35.0	37.0	32.0	37.0
7	35.217687854221836	37.0	35.0	37.0	32.0	37.0
8	35.26109717989988	37.0	35.0	37.0	32.0	37.0
9	37.03565548248086	39.0	37.0	39.0	33.0	39.0
10	36.84260215725371	39.0	37.0	39.0	33.0	39.0
11	36.88698718102711	39.0	37.0	39.0	33.0	39.0
12	36.80392348900769	39.0	37.0	39.0	33.0	39.0
13	36.91789167903229	39.0	37.0	39.0	33.0	39.0
14	37.89842206703859	40.0	37.0	41.0	33.0	41.0
15	37.856649992331164	39.0	37.0	41.0	33.0	41.0
16	37.86296061542897	39.0	37.0	41.0	33.0	41.0
17	37.74425807214622	39.0	37.0	41.0	32.0	41.0
18	37.88669890658984	40.0	37.0	41.0	33.0	41.0
19	37.90341143229893	40.0	37.0	41.0	33.0	41.0
20	37.93363959412437	40.0	37.0	41.0	33.0	41.0
21	37.952375584633494	40.0	37.0	41.0	33.0	41.0
22	37.75828558018002	39.0	37.0	41.0	32.0	41.0
23	37.8566666235487	39.0	37.0	41.0	33.0	41.0
24	37.69670572538903	39.0	37.0	41.0	32.0	41.0
25	37.63694421704847	39.0	37.0	41.0	32.0	41.0
26	37.59345727902193	39.0	37.0	41.0	32.0	41.0
27	37.43124562275594	39.0	36.0	41.0	32.0	41.0
28	37.54534316669469	39.0	37.0	41.0	32.0	41.0
29	37.49957590395287	39.0	37.0	41.0	32.0	41.0
30	37.44351206964415	39.0	37.0	41.0	32.0	41.0
31	37.44611947497094	39.0	37.0	41.0	32.0	41.0
32	37.273492980702244	39.0	36.0	40.0	31.0	41.0
33	37.21780981648375	39.0	36.0	40.0	31.0	41.0
34	37.14595926090869	39.0	36.0	40.0	31.0	41.0
35	37.15236782339864	39.0	36.0	40.0	31.0	41.0
36	37.14918756502344	39.0	36.0	40.0	31.0	41.0
37	37.09140517156949	39.0	36.0	40.0	31.0	41.0
38	37.05932355294548	39.0	36.0	40.0	31.0	41.0
39	36.92571019918655	39.0	36.0	40.0	31.0	41.0
40	36.762216091257336	39.0	35.0	40.0	30.0	41.0
41	36.7692566400136	39.0	35.0	40.0	30.0	41.0
42	36.71647839512447	39.0	35.0	40.0	30.0	41.0
43	35.950045366265606	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	16.0
10	27.0
11	26.0
12	21.0
13	20.0
14	10.0
15	15.0
16	16.0
17	20.0
18	33.0
19	34.0
20	72.0
21	130.0
22	271.0
23	483.0
24	925.0
25	1470.0
26	2572.0
27	3790.0
28	5447.0
29	7818.0
30	10782.0
31	14376.0
32	18517.0
33	23530.0
34	30726.0
35	40813.0
36	56013.0
37	81442.0
38	133413.0
39	108320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.00012565808804	24.407235688375334	17.297390192386228	26.2952484611504
2	12.189204122324453	20.66225508222289	43.175195093421245	23.97334570203141
3	12.837821606169072	30.19138835556065	36.202279955132674	20.76851008313761
4	10.346834802116229	20.641558455957764	34.924078491955115	34.087528249970894
5	11.434516428871055	38.47650655731949	35.92176675271782	14.167210261091636
6	25.989049267210074	40.461534765712344	17.193537478448714	16.355878488628868
7	21.83900611844014	34.976189640229805	21.641094629779857	21.543709611550195
8	24.474499723736997	34.74464613388869	23.622242220748	17.15861192162631
9	20.821914770553875	14.67704947417634	18.08552511221452	46.41551064305526
10	13.56146436022478	28.426261801234777	33.16541963333709	24.846854205203353
11	28.168293138144435	22.449926175873276	25.74493995206514	23.63684073391715
12	17.61061145595222	27.745675421462774	26.5171828195827	28.126530303002305
13	29.5058126105283	18.853702571001442	27.04365325020188	24.596831568268378
14	21.537057124536403	24.327775426821717	24.044859937429663	30.09030751121221
15	24.364548896703507	29.609480533159875	20.13744777335716	25.888522796779455
16	27.134016198805877	26.08994532025257	21.729424874018527	25.046613606923025
17	18.23354294827137	34.901903535242475	22.90266487542294	23.961888641063215
18	23.583620837806823	23.635916777387457	23.43597258436185	29.344489800443867
19	20.718986013146054	28.04152630227053	25.50674395871023	25.732743725873185
20	18.97030588504872	25.153053399143676	27.265402817328248	28.611237898479352
21	23.828654109481455	23.71704016069452	24.895454318665216	27.55885141115881
22	18.133570851758567	32.55320603676238	28.6071724897487	20.70605062173035
23	22.08052835530194	23.11443571202862	25.615955620519966	29.189080312149475
24	21.621137168738485	29.203863616624563	28.633597646497925	20.541401568139023
25	18.672052717263757	31.216056146990397	25.992929884634787	24.118961251111056
26	21.81830949217501	25.321583070159715	25.07082126800098	27.78928616966429
27	17.60654604722157	32.04004058017079	30.720630655768904	19.632782716838737
28	17.699496074108705	23.09706532927039	28.540278036998917	30.663160559621993
29	17.902027345417455	24.203041295313138	31.792789812824886	26.102141546444525
30	17.96485638943659	25.146585703435825	32.493888027556075	24.394669879571506
31	19.87042433627583	23.14289357314317	28.275841678200724	28.71084041238028
32	21.34210229677114	28.67924109906477	31.015187997435095	18.963468606728988
33	18.04745810319116	24.730805265073887	29.85894879617704	27.36278783555791
34	19.058081755369574	30.293762739050656	29.95097486653448	20.697180639045293
35	19.816834857553623	27.39309360973185	34.235361294721805	18.554710237992722
36	22.564681576861172	24.74392544779553	23.204429078020738	29.48696389732256
37	20.13985006033436	27.01685851084078	29.282399921648487	23.560891507176372
38	20.756313856945656	23.538162176545917	29.95929047530172	25.746233491206706
39	25.168575868842524	24.94497838865677	26.153328738189526	23.73311700431118
40	18.626594056002855	22.380814227452227	37.65898981984696	21.333601896697964
41	21.35707039255217	26.221147147469004	25.53852806333168	26.883254396647143
42	22.07498461612378	23.083390772630928	33.93137959645275	20.910245014792544
43	22.064821094297155	22.57872571611251	32.875482074319365	22.480971115270968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	11.5
2	13.0
3	48.5
4	84.0
5	84.0
6	113.0
7	142.0
8	157.0
9	172.0
10	275.0
11	378.0
12	378.0
13	560.5
14	743.0
15	1617.0
16	2491.0
17	2806.0
18	3121.0
19	3121.0
20	3363.5
21	3606.0
22	4567.0
23	5528.0
24	7279.5
25	9031.0
26	9031.0
27	11074.5
28	13118.0
29	15550.0
30	17982.0
31	20539.0
32	23096.0
33	23096.0
34	25800.5
35	28505.0
36	30341.0
37	32177.0
38	34360.5
39	36544.0
40	36544.0
41	37201.5
42	37859.0
43	37620.0
44	37381.0
45	36744.5
46	36108.0
47	36108.0
48	36766.5
49	37425.0
50	54698.5
51	71972.0
52	49318.5
53	26665.0
54	26665.0
55	40146.5
56	53628.0
57	42960.5
58	32293.0
59	21496.0
60	10699.0
61	10699.0
62	9160.0
63	7621.0
64	6036.0
65	4451.0
66	3555.5
67	2660.0
68	2660.0
69	2185.5
70	1711.0
71	1446.5
72	1182.0
73	1226.5
74	1271.0
75	1271.0
76	1092.0
77	913.0
78	656.5
79	400.0
80	254.0
81	108.0
82	108.0
83	72.0
84	36.0
85	27.5
86	19.0
87	13.5
88	8.0
89	8.0
90	4.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	541151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.587494768685715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8361885358267	31.122495992420703
2	13.854903791095932	11.523814778656918
3	4.529350888581738	5.65093069153305
4	1.9989581578551074	3.325266477304836
5	1.0647252292253786	2.213962745024907
6	0.6990918292930555	1.7444086676133521
7	0.4660811764997682	1.3568203942626895
8	0.33854815305911423	1.1263495635435299
9	0.2495673415384184	0.9340992459597398
>10	1.7264854924833646	14.026184314364103
>50	0.15933362461582973	4.547080148217121
>100	0.07007108981584968	4.8839627699600285
>500	0.001338938021958911	0.3560004785764958
>1k	0.002677876043917822	1.8306739938477468
>5k	8.926253479726074E-4	2.4697301188419467
>10k+	0.0017852506959452148	12.888219619872856
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	21303	3.936609190410809	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	19323	3.570722404652306	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	18793	3.472783012504828	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	10018	1.8512393028932774	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	7891	1.458188195161794	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	5415	1.0006449216577258	RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	2264	0.4183675166450769	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	2015	0.37235448146635597	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1573	0.2906767242414779	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.25796866309033895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.25408804566562754	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	1240	0.22914121936391135	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	796	0.14709387952715602	Illumina PCR Primer Index 11 (95% over 24bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	616	0.11383144445820112	Illumina PCR Primer Index 11 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	1.847913059386382E-4	0.0
17	0.0	0.0	0.0	1.847913059386382E-4	0.0
18	0.0	0.0	0.0	3.695826118772764E-4	0.0
19	0.0	0.0	0.0	7.391652237545528E-4	0.0
20	0.0	0.0	0.0	7.391652237545528E-4	0.0
21	0.0	0.0	0.0	7.391652237545528E-4	0.0
22	0.0	0.0	0.0	7.391652237545528E-4	0.0
23	0.0	0.0	0.0	9.23956529693191E-4	0.0
24	0.0	0.0	0.0	9.23956529693191E-4	0.0
25	0.0	0.0	0.0	0.0011087478356318291	0.0
26	0.0	0.0	0.0	0.0011087478356318291	0.0
27	0.0	0.0	0.0	0.00203270436532502	0.0
28	0.0	0.0	0.0	0.005358947872220508	0.0
29	0.0	0.0	0.0	0.011457060968195569	0.0
30	0.0	0.0	0.0	0.02365328716014569	0.0
31	0.0	0.0	0.0	0.06467695707852338	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2425	0.0	35.321648	1
CGTCTTC	1135	0.0	33.088108	37
TTATACA	2645	0.0	32.24386	2
TTACTCG	35	2.3859906E-5	31.714285	19
TATACAC	2865	0.0	30.155323	3
GATACAC	40	5.9344093E-5	27.750002	3
ATTCCGG	40	5.9344093E-5	27.750002	37
GCTCTAG	90	5.456968E-12	26.722221	1
CGTTACT	35	8.863308E-4	26.42857	17
ACCGTTA	35	8.863308E-4	26.42857	8
CGCCGAG	50	9.079487E-6	25.899998	2
GTATCAA	575	0.0	23.808697	1
CCGTCTT	3150	0.0	23.668253	37
GGTATCA	205	0.0	23.463413	1
GCCGTCT	3215	0.0	23.189734	36
TCGGCGT	40	0.0019298736	23.125002	11
CGCACCA	50	2.699543E-4	22.199999	18
CCGCCTA	50	2.699543E-4	22.199999	15
GCCGAGC	60	3.7215977E-5	21.583332	3
CGGCGTA	45	0.0038232314	20.555555	12
>>END_MODULE