FastQCFastQC Report
Fri 10 Feb 2017
ERR1630967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682426
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG164082.404363256968521No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT131721.9301726487560555No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT111441.6329975704325452RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC92331.3529672081661601No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC41820.6128136970162332No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC40640.5955224449244314RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG21760.31886241145560107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15360.2250793492627772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.21145149803788252No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA14320.20983960165644333No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13570.1988493990557218No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT12800.18756612438564768No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11760.17232637677931378No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10130.14844100312707897No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9090.1332012555207451No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC8770.12851210241110392No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8110.11884072412246896No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA7920.11605653946361949No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT7850.11503078722088547No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA7780.11400503497815148No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7700.11283274670074118No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7370.1079970575564237No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG7090.10389404858548766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16900.034.3727841
TTATACA18400.031.5706522
TATACAC19400.030.4201033
CGTCTTC5600.030.39285737
TCGCCGA250.005494797629.61
GTATCAA9600.025.8229181
CCGTCTT16400.025.04268537
GCCGTCT16650.024.77777936
GGTATCA2900.024.2413791
CTGATAC1155.456968E-1222.521741
GAACCGT502.7005433E-422.26
GATACAC1500.020.9666673
TACGGTC450.00382423920.55555510
GTATACG450.00382423920.5555551
TGCCGTC22100.018.7511335
AACCGTG500.00703202418.57
TGAACCG500.00703202418.55
GGTATAC609.231572E-418.53
GTGAGTT500.00703202418.520
TTACACC609.231572E-418.53
Produced by FastQC (version 0.11.5)