FastQCFastQC Report
Fri 10 Feb 2017
ERR1630966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451409
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG160983.566167267378364No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT121082.682268186943548TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC88091.9514453632958138No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT72271.6009871314041146No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC42480.941053457064436RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC41720.9242172841037729No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG21440.47495730036397144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.36928816217665134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16460.3646360617533102No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA13070.28953786920508895No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT11910.2638405525809189No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11050.24478909370437896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6010.13313868354419164No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT4870.10788442410319687No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4830.10699830973684618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC5350.034.57943737
CTTATAC9700.034.520621
ATCGCTA303.5960475E-430.83333210
TTATACA11300.029.6327442
GAATGCG250.00549285229.629
GGTATCA2750.028.2545431
TATACAC12700.027.2401583
CCGTCTT17550.025.50997237
GCCGTCT18250.024.53150736
AAGACTG903.812602E-922.611115
GTATCAA7150.022.5104891
ATACACA30200.020.582781
TACACAT30800.020.3019472
TTAAGAC555.1364483E-420.1818183
CTCTAAT555.1364483E-420.1818181
TGCCGTC22600.019.80973435
TATACTG759.2453465E-619.7333345
ATGCCGT23950.018.69311134
GCTATCC500.00702842718.513
CCCACCG500.00702842718.519
Produced by FastQC (version 0.11.5)