FastQCFastQC Report
Fri 10 Feb 2017
ERR1630965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085803
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT181091.6677979338793503No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG141891.306774801690546No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT87080.8019871007908432Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC76670.706113355737643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37080.3414984117745116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33170.30548819629343443No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC33150.30530400081782794No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC27820.256215906568687Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24200.22287652548390455No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG19230.17710394979568117No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17940.16522334161905983No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15960.14698798953401307No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15190.13989646372316158No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC14940.1375940202780799No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13580.12506872793683568No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13170.12129272068690178No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT13170.12129272068690178No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13140.12101642747349196No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA13120.12083223199788544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13110.12074013426008218No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT13070.1203717433088691No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12830.11816139760159072No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA11390.10489932335792035No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT11280.10388624824208444No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11180.10296527086405176No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC10990.10121541384578969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21250.033.6047061
GATTACG950.031.1578961
TTATACA24100.030.1680492
CGTCTTC3950.029.50632937
TATACAC25150.029.1292253
TTACGGG1100.028.5909083
GGTATCA6600.027.4696961
ATTACGG1150.025.7391282
GCCGTCT12100.025.38016536
CCGTCTT12200.025.02049337
GTATCAA20850.024.4892081
ACGTACT450.003825674520.55555513
TGCCGTC15750.019.61587335
ATACACA40650.018.5227554
GACCGTT801.616536E-518.57
GTTACGG701.2191187E-418.517
GCGGGTA609.236368E-418.522
TACACAT40800.018.4546555
GTACTGG6600.018.2196961
ATGCCGT17100.018.06725134
Produced by FastQC (version 0.11.5)