##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630965.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1085803
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353168116131563	31.0	31.0	34.0	28.0	34.0
2	31.342212169242487	31.0	31.0	34.0	28.0	34.0
3	30.78909065456625	31.0	31.0	34.0	26.0	34.0
4	34.983590025078215	37.0	35.0	37.0	32.0	37.0
5	35.180766676828114	37.0	35.0	37.0	32.0	37.0
6	35.38298291679062	37.0	35.0	37.0	33.0	37.0
7	35.39153511272303	37.0	35.0	37.0	33.0	37.0
8	35.40577434396479	37.0	35.0	37.0	33.0	37.0
9	37.165408458072044	39.0	37.0	39.0	33.0	39.0
10	36.998523673263016	39.0	37.0	39.0	33.0	39.0
11	37.057142041420036	39.0	37.0	39.0	33.0	39.0
12	36.99457636422076	39.0	37.0	39.0	33.0	39.0
13	37.05821498006544	39.0	37.0	39.0	33.0	39.0
14	38.16444051084773	40.0	37.0	41.0	33.0	41.0
15	38.17368988665532	40.0	37.0	41.0	33.0	41.0
16	38.16067647630371	40.0	37.0	41.0	33.0	41.0
17	38.141873802153796	40.0	37.0	41.0	33.0	41.0
18	38.18970476228193	40.0	38.0	41.0	33.0	41.0
19	38.20771079099984	40.0	38.0	41.0	33.0	41.0
20	38.2091337010489	40.0	38.0	41.0	34.0	41.0
21	38.168639246714186	40.0	37.0	41.0	33.0	41.0
22	38.09206918750455	40.0	37.0	41.0	33.0	41.0
23	38.11231595418322	40.0	37.0	41.0	33.0	41.0
24	38.051385932807335	40.0	37.0	41.0	33.0	41.0
25	38.0140826650875	40.0	37.0	41.0	33.0	41.0
26	37.91568452104111	40.0	37.0	41.0	33.0	41.0
27	37.821126852661116	40.0	37.0	41.0	33.0	41.0
28	37.824826418788675	40.0	37.0	41.0	33.0	41.0
29	37.771500907623206	40.0	37.0	41.0	33.0	41.0
30	37.66625529677115	40.0	37.0	41.0	32.0	41.0
31	37.64935812481638	40.0	37.0	41.0	32.0	41.0
32	37.54349085423415	39.0	37.0	41.0	32.0	41.0
33	37.52936029832299	39.0	37.0	41.0	32.0	41.0
34	37.461518341724975	39.0	36.0	41.0	32.0	41.0
35	37.42792384990648	39.0	36.0	41.0	32.0	41.0
36	37.37441690619753	39.0	36.0	41.0	32.0	41.0
37	37.318951043605516	39.0	36.0	41.0	31.0	41.0
38	37.257507116852686	39.0	36.0	41.0	31.0	41.0
39	37.18114796146262	39.0	36.0	41.0	31.0	41.0
40	37.101651957122975	39.0	36.0	40.0	31.0	41.0
41	37.04367643117582	39.0	36.0	40.0	31.0	41.0
42	36.983403987647854	39.0	36.0	40.0	31.0	41.0
43	36.26321809757387	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	14.0
11	14.0
12	13.0
13	8.0
14	6.0
15	8.0
16	10.0
17	11.0
18	19.0
19	62.0
20	110.0
21	221.0
22	449.0
23	821.0
24	1539.0
25	2616.0
26	4287.0
27	6605.0
28	9464.0
29	13936.0
30	18916.0
31	25101.0
32	33092.0
33	42591.0
34	56783.0
35	76322.0
36	106670.0
37	156762.0
38	268119.0
39	261225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.99012988543962	21.877633419690312	15.082478129089715	24.049758565780348
2	13.98743602660888	22.591851376354644	41.08387985665908	22.3368327403774
3	15.020035862859102	28.35164389857092	34.713203039593736	21.91511719897624
4	12.168137314043156	19.454541938086376	37.574956046354636	30.802364701515838
5	12.508438455226226	38.56178330691663	35.518689854421105	13.411088383436038
6	27.298966755479587	39.77231597260277	18.067550006769185	14.861167265148467
7	24.233585650435668	33.44032020541479	22.456836092735056	19.86925805141448
8	24.145079724406727	35.48590305976314	22.432061801265977	17.936955414564153
9	24.078308864499363	15.48724768673507	19.4722247037446	40.96221874502097
10	14.47048866138701	29.04385049590027	33.46463400819486	23.021026834517862
11	31.097261657961894	23.274571906690255	25.54818875983949	20.079977675508356
12	20.81325986389796	26.242974093827332	29.214599701787524	23.72916634048718
13	28.50148691797683	21.141496201428804	27.52607977690244	22.830937103691923
14	21.379568853650248	22.525080516447275	26.91482709110216	29.18052353880032
15	24.091939329694245	28.185960068262844	23.75992698491347	23.96217361712944
16	24.24795289753298	27.910864125444483	23.33950081184156	24.501682165180977
17	21.494230537215316	30.037216695846297	24.84714077968103	23.621411987257357
18	23.441637203065383	26.126746748719608	26.498545316231397	23.933070731983612
19	24.36648268608578	27.189923033920515	27.12877013601915	21.31482414397455
20	23.381405282542044	26.07084342187303	26.527556103639427	24.0201951919455
21	23.881403901075977	26.08668423277519	25.75632964727487	24.275582218873957
22	21.69371423729719	30.456537696064572	25.76471054141497	22.085037525223267
23	22.519370456703474	26.03280705616028	26.575999513723946	24.871822973412307
24	24.27760836910563	27.28607307218713	27.18605492893278	21.250263629774462
25	23.185329198758893	27.972661707510476	25.832218183224764	23.009790910505863
26	23.753940631956258	25.8615052638462	27.13613795504341	23.248416149154128
27	22.756153740595668	29.3773364044859	26.080789977555778	21.785719877362652
28	20.55078131115865	26.314165645149256	27.278336862211656	25.856716181480433
29	23.324765173793036	26.592669204266333	27.955623626016873	22.126941995923755
30	21.703200304290927	28.184118113506777	26.9978071528629	23.11487442933939
31	22.8331474493992	25.475431546974914	26.96630972653419	24.725111277091703
32	24.69158770053131	26.300350984478772	26.988781574558185	22.019279740431735
33	21.23101520257358	25.500942620346418	28.234403478347364	25.03363869873264
34	21.254684321189018	27.510423161475885	28.123978290721247	23.11091422661385
35	23.32568615117107	26.37808147518472	28.79177898753273	21.50445338611148
36	22.63154550134785	26.281931436918114	25.56062195444293	25.525901107291098
37	23.224378639587474	26.80513868537847	27.243984406011034	22.72649826902302
38	22.660187897804665	25.474050080907862	28.15621249895239	23.70954952233508
39	23.88121970560037	25.682743554770067	26.743985787477104	23.692050952152464
40	21.652270255285718	25.507573657468253	30.344731042371407	22.495425044874622
41	22.559801363599107	26.216818336291205	26.670123401758882	24.553256898350806
42	23.70604980829856	25.048005945829953	28.448530718739956	22.797413527131532
43	23.046998396578385	24.92220043599069	28.835157022038064	23.19564414539286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	17.0
2	27.0
3	88.5
4	150.0
5	150.0
6	212.0
7	274.0
8	304.5
9	335.0
10	517.5
11	700.0
12	700.0
13	1078.0
14	1456.0
15	3701.5
16	5947.0
17	6683.0
18	7419.0
19	7419.0
20	7643.5
21	7868.0
22	9638.5
23	11409.0
24	14303.0
25	17197.0
26	17197.0
27	21250.0
28	25303.0
29	31980.5
30	38658.0
31	41402.5
32	44147.0
33	44147.0
34	48715.0
35	53283.0
36	56052.0
37	58821.0
38	64283.5
39	69746.0
40	69746.0
41	73920.5
42	78095.0
43	73887.5
44	69680.0
45	73166.0
46	76652.0
47	76652.0
48	78143.5
49	79635.0
50	81971.5
51	84308.0
52	101620.0
53	118932.0
54	118932.0
55	91292.5
56	63653.0
57	64754.0
58	65855.0
59	53211.5
60	40568.0
61	40568.0
62	32370.5
63	24173.0
64	19993.0
65	15813.0
66	13143.0
67	10473.0
68	10473.0
69	8451.0
70	6429.0
71	5325.0
72	4221.0
73	3250.5
74	2280.0
75	2280.0
76	1728.0
77	1176.0
78	891.5
79	607.0
80	425.5
81	244.0
82	244.0
83	186.0
84	128.0
85	106.0
86	84.0
87	59.5
88	35.0
89	35.0
90	23.5
91	12.0
92	7.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1085803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.22880179113942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2413701380986	34.255094833581786
2	12.154812510504193	10.50875961650095
3	3.4916549404846284	4.528201780357882
4	1.4544936598965854	2.515040725205538
5	0.7767411436683993	1.6788794471332091
6	0.46918186638631404	1.2169301943606496
7	0.3366355687707359	1.0186646594766338
8	0.2478429847403329	0.8571164210131392
9	0.18244277873212594	0.7098104448032201
>10	1.2820676184862434	11.112621317847038
>50	0.1791415683886099	5.445990183632069
>100	0.16591479978376486	14.292417049620415
>500	0.010662904855055517	3.1161698771997375
>1k	0.006184484815932201	4.257192141269944
>5k	4.265161942022207E-4	1.509593566627587
>10k+	4.265161942022207E-4	2.977517741370248
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	18109	1.6677979338793503	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	14189	1.306774801690546	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	8708	0.8019871007908432	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	7667	0.706113355737643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3708	0.3414984117745116	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3317	0.30548819629343443	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	3315	0.30530400081782794	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	2782	0.256215906568687	Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2420	0.22287652548390455	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1923	0.17710394979568117	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1794	0.16522334161905983	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1596	0.14698798953401307	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1519	0.13989646372316158	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1494	0.1375940202780799	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1358	0.12506872793683568	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1317	0.12129272068690178	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1317	0.12129272068690178	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1314	0.12101642747349196	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	1312	0.12083223199788544	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1311	0.12074013426008218	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1307	0.1203717433088691	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1283	0.11816139760159072	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1139	0.10489932335792035	No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT	1128	0.10388624824208444	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1118	0.10296527086405176	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1099	0.10121541384578969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.209773780326634E-5	0.0
10	0.0	9.209773780326634E-5	0.0	1.8419547560653268E-4	0.0
11	0.0	9.209773780326634E-5	0.0	2.76293213409799E-4	0.0
12	0.0	9.209773780326634E-5	0.0	2.76293213409799E-4	0.0
13	0.0	9.209773780326634E-5	0.0	2.76293213409799E-4	0.0
14	0.0	9.209773780326634E-5	0.0	2.76293213409799E-4	0.0
15	0.0	9.209773780326634E-5	0.0	2.76293213409799E-4	0.0
16	0.0	9.209773780326634E-5	0.0	3.6839095121306536E-4	0.0
17	0.0	9.209773780326634E-5	0.0	3.6839095121306536E-4	0.0
18	0.0	9.209773780326634E-5	0.0	3.6839095121306536E-4	0.0
19	0.0	9.209773780326634E-5	0.0	3.6839095121306536E-4	0.0
20	0.0	9.209773780326634E-5	0.0	4.604886890163317E-4	0.0
21	0.0	9.209773780326634E-5	0.0	6.446841646228644E-4	0.0
22	0.0	9.209773780326634E-5	0.0	0.0011972705914424623	0.0
23	0.0	9.209773780326634E-5	0.0	0.0014735638048522614	0.0
24	0.0	9.209773780326634E-5	0.0	0.002210345707278392	0.0
25	0.0	9.209773780326634E-5	0.0	0.0023945411828849247	0.0
26	0.0	9.209773780326634E-5	0.0	0.0025787366584914574	0.0
27	0.0	9.209773780326634E-5	0.0	0.003868104987737186	0.0
28	0.0	9.209773780326634E-5	0.0	0.008933480566916835	0.0
29	0.0	9.209773780326634E-5	0.0	0.02109038195694799	0.0
30	0.0	9.209773780326634E-5	0.0	0.03923363630419146	0.0
31	0.0	9.209773780326634E-5	0.0	0.08334845271195604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2125	0.0	33.604706	1
GATTACG	95	0.0	31.157896	1
TTATACA	2410	0.0	30.168049	2
CGTCTTC	395	0.0	29.506329	37
TATACAC	2515	0.0	29.129225	3
TTACGGG	110	0.0	28.590908	3
GGTATCA	660	0.0	27.469696	1
ATTACGG	115	0.0	25.739128	2
GCCGTCT	1210	0.0	25.380165	36
CCGTCTT	1220	0.0	25.020493	37
GTATCAA	2085	0.0	24.489208	1
ACGTACT	45	0.0038256745	20.555555	13
TGCCGTC	1575	0.0	19.615873	35
ATACACA	4065	0.0	18.522755	4
GACCGTT	80	1.616536E-5	18.5	7
GTTACGG	70	1.2191187E-4	18.5	17
GCGGGTA	60	9.236368E-4	18.5	22
TACACAT	4080	0.0	18.454655	5
GTACTGG	660	0.0	18.219696	1
ATGCCGT	1710	0.0	18.067251	34
>>END_MODULE