FastQCFastQC Report
Fri 10 Feb 2017
ERR1630964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413293
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG98192.375796347869429No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT85332.0646369524768144RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT73661.7822706893172642No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC51911.2560096590070482No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC27600.6678071005315841No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC27080.655225227623018RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.573685012811734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22380.5415044532571324No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA12890.3118852726758014No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG12530.3031747452775634No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA8210.1986484164987067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8170.19768058012112474No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7640.18485674811816313No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT7440.1800175662302531No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6260.15146639309158394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA200.001840572337.01
GCAACGC251.2322576E-437.03
TATTCGC200.001840572337.03
CTTATAC9650.032.974091
CGTCTTC3250.030.7384637
TACTCGA250.005492320729.65
CTCGAAC250.005492320729.628
GGTATCA3400.028.2941171
CCGTCTT13200.028.17045437
TTATACA11400.028.0745622
GATTACG405.9310667E-527.751
TATACAC11550.027.7099573
GCCGTCT13450.027.6468436
GTATCAA8750.022.6228561
TGCCGTC16800.022.13392835
ATGCCGT17700.021.00847434
AGCAACG450.003821724620.5555552
ATTACGG555.135437E-420.1818182
ATTGAGC2250.019.7333346
CTAGCCA851.2413639E-619.5882349
Produced by FastQC (version 0.11.5)